Manipulation and capture of Aβ amyloid fibrils and monomers by DC insulator gradient dielectrophoresis (DC-iGDEP)

Staton, Sarah J. R.; Jones, Paul V.; Ku, Ginger; Gilman, S. Douglass; Kheterpal, Indu; Hayes, Mark A.

doi:10.1039/c2an35138b

Cited by 31 publications

(53 citation statements)

References 22 publications

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“…Microfluidic devices have the potential to improve each of these figures of merit and provide increased portability. A wide range of bioparticles can be addressed, including animal cells 1 , organelles 2 , proteins 3, 4 , DNA 5–7 and bacteria. 8–11 …”

Section: Introductionmentioning

confidence: 99%

“…28, 45, 46 Trapping DEP leads to the isolation and concentration of analytes near the constriction points in the microchannels. 1–3, 6, 8, 10, 11, 25, 26, 28, 35 Separation can be achieved as a bipurification, where one analyte is trapped and other analytes continue to flow through the microchannel 10 , or multiple analytes can be trapped in the same microchannel. 11 Deflection techniques can also be used to achieve separations.…”

Section: Introductionmentioning

confidence: 99%

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Refinement of insulator-based dielectrophoresis

Crowther

Hayes

2017

Analyst

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The ability to separate analytes with increasingly similar properties drives the field of separation science. One way to achieve such separations is using trapping and streaming dielectrophoresis (DEP), which directly exploits the subtle differences in the electrophysical properties of analytes. The non-uniform fields necessary for DEP can be formed using various insulator shapes in microchannels. Current insulator shapes include triangles, diamonds, circles, and rectangles. However, all of these insulators pose problems for trapping, streaming, and sorting (deflection) as the induced fields/gradients are not behaviorally consistent across the lateral dimension. This leads to analytes experiencing different forces depending on their pathline in the microchannel and result in low resolution separations. Based on an iterative process that explored approximately 40 different insulator shapes, a design was chosen that indicated improved particle streamlines, better trapping efficiency, and consistent electrical environments across the lateral dimension. The design was assessed by simulations where the electric field, gradient of the electric field squared, and the ratio of the two were plotted. The improved design includes a unique new multi-length scale element. The multi-length scale structure streamlines the analyte(s) and improves homogeneity in the lateral dimension, while still achieving high gradients necessary for analyte separation using DEP. The design is calculated to keep analytes on the centerline which should improve resolution, and eliminate extraneous trapping zones. Behaviors consistent with the features of the simulations were observed in proof of principle experiments using representative test probes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Refinement of insulator-based dielectrophoresis

Crowther

Hayes

2017

Analyst

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“…[31] have recently reviewed the different devices reported for protein iDEP. Those iDEP devices for protein studies include nanoconstrictions [15], post-arrays [2,4], sawtooth [44] and nanostructures [9]. …”

Section: Advances In Protein Depmentioning

confidence: 99%

“…Staton et al . reported a novel method for concentration of Aβ amyloid fibrils [44]. Furthermore, the influence of device scaling in iDEP geometries for protein manipulation was investigated by Chaurey et al .…”

Section: Advances In Protein Depmentioning

confidence: 99%

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Protein Dielectrophoresis and The Link to Dielectric Properties

Camacho-Alanis

Ros²

2015

Bioanalysis

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There is a growing interest in protein dielectrophoresis (DEP) for biotechnological and pharmaceutical applications. However, the DEP behavior of proteins is still not well understood which is important for successful protein manipulation. In this paper, we elucidate the information gained in dielectric spectroscopy (DS) and electrochemical impedance spectroscopy (EIS) and how these techniques may be of importance for future protein DEP manipulation. EIS and DS can be used to determine the dielectric properties of proteins predicting their DEP behavior. Basic principles of EIS and DS are discussed and related to protein DEP through examples from previous studies. Challenges of performing DS measurements as well as potential designs to incorporate EIS and DS measurements in DEP experiments are also discussed.

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Dielectrophoretic Studies of Bioparticles

2017

Dielectrophoresis

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Manipulation and capture of Aβ amyloid fibrils and monomers by DC insulator gradient dielectrophoresis (DC-iGDEP)

Cited by 31 publications

References 22 publications

Refinement of insulator-based dielectrophoresis

Refinement of insulator-based dielectrophoresis

Protein Dielectrophoresis and The Link to Dielectric Properties

Dielectrophoretic Studies of Bioparticles

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