Protein Dielectrophoresis and The Link to Dielectric Properties

Camacho-Alanis, Fernanda; Ros, Alexandra

doi:10.4155/bio.14.306

Cited by 20 publications

(15 citation statements)

References 146 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2c,d ). Here, sharp changes in the fluorescence intensities owing to captured viruses and proteins were observed over narrow and low frequency ranges (500 to 2000 Hz and 300 to 800 Hz for viruses and proteins, respectively), which is typical of EO spectra rather than DEP as DEP behavior generally changes with wider frequency ranges 40 , 41 . Table 2 shows the obtained AC electrical potentials and frequencies for the particles and media.…”

Section: Resultsmentioning

confidence: 83%

Rapid and selective concentration of bacteria, viruses, and proteins using alternating current signal superimposition on two coplanar electrodes

Han

Woo

Bhardwaj

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Dielectrophoresis (DEP) is usually effective close to the electrode surface. Several techniques have been developed to overcome its drawbacks and to enhance dielectrophoretic particle capture. Here we present a simple technique of superimposing alternating current DEP (high-frequency signals) and electroosmosis (EO; low-frequency signals) between two coplanar electrodes (gap: 25 μm) using a lab-made voltage adder for rapid and selective concentration of bacteria, viruses, and proteins, where we controlled the voltages and frequencies of DEP and EO separately. This signal superimposition technique enhanced bacterial capture (Escherichia coli K-12 against 1-μm-diameter polystyrene beads) more selectively (>99%) and rapidly (~30 s) at lower DEP (5 Vpp) and EO (1.2 Vpp) potentials than those used in the conventional DEP capture studies. Nanometer-sized MS2 viruses and troponin I antibody proteins were also concentrated using the superimposed signals, and significantly more MS2 and cTnI-Ab were captured using the superimposed signals than the DEP (10 Vpp) or EO (2 Vpp) signals alone (p < 0.035) between the two coplanar electrodes and at a short exposure time (1 min). This technique has several advantages, such as simplicity and low cost of electrode fabrication, rapid and large collection without electrolysis.

show abstract

Section: Resultsmentioning

confidence: 83%

Rapid and selective concentration of bacteria, viruses, and proteins using alternating current signal superimposition on two coplanar electrodes

Han

Woo

Bhardwaj

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Dielectrophoresis (DEP) is the migration of polarized dielectric particles in a non-uniform electric field and has attracted intense interest in biotechnology regarding the separation and concentration of viruses [ 29 ], bacteria [ 30 ], micro algae [ 31 ], DNA [ 32 ], and protein [ 33 ], as well as its compatibility with micro fluidic systems and lab-on-a-chip (LOC) devices [ 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

An Approach to Ring Resonator Biosensing Assisted by Dielectrophoresis: Design, Simulation and Fabrication

et al. 2020

View full text Add to dashboard Cite

The combination of extreme miniaturization with a high sensitivity and the potential to be integrated in an array form on a chip has made silicon-based photonic microring resonators a very attractive research topic. As biosensors are approaching the nanoscale, analyte mass transfer and bonding kinetics have been ascribed as crucial factors that limit their performance. One solution may be a system that applies dielectrophoretic forces, in addition to microfluidics, to overcome the diffusion limits of conventional biosensors. Dielectrophoresis, which involves the migration of polarized dielectric particles in a non-uniform alternating electric field, has previously been successfully applied to achieve a 1000-fold improved detection efficiency in nanopore sensing and may significantly increase the sensitivity in microring resonator biosensing. In the current work, we designed microring resonators with integrated electrodes next to the sensor surface that may be used to explore the effect of dielectrophoresis. The chip design, including two different electrode configurations, electric field gradient simulations, and the fabrication process flow of a dielectrohoresis-enhanced microring resonator-based sensor, is presented in this paper. Finite element method (FEM) simulations calculated for both electrode configurations revealed ∇E2 values above 1017 V2m−3 around the sensing areas. This is comparable to electric field gradients previously reported for successful interactions with larger molecules, such as proteins and antibodies.

show abstract

“…The DEP force on dielectric microspheres depends on the polarizability of the microspheres, the polarizability of the ssDNA bound to the microspheres, and the polarizability of the molecules in the solution. The polarization mechanism of DNA is still not fully understood [36,48]. We are currently improving this method by designing a microstructured electrode array that is optimized for negative DEP spectroscopy.…”

Section: Discussionmentioning

confidence: 99%

“…This phenomenon is denoted negative DEP [34][35][36][37]. Since the DEP force is strongly dependent on the characteristics of the particles and the molecules bound to their surfaces, DEP has been used to isolate, concentrate, or separate different types of target particles [38][39][40][41][42][43]. However, the physics behind the frequency-dependence of the DEP force on the molecular structures is still not well understood [40,44,45].…”

Section: Theorymentioning

confidence: 99%

Nucleotide Identification in DNA Using Dielectrophoresis Spectroscopy

et al. 2019

View full text Add to dashboard Cite

We show that negative dielectrophoresis (DEP) spectroscopy is an effective transduction mechanism of a biosensor for the detection of single nucleotide polymorphism (SNP) in a short DNA strand. We observed a frequency dependence of the negative DEP force applied by interdigitated electrodes to polystyrene microspheres (PM) with respect to changes in both the last and the second-to-last nucleotides of a single-strand DNA bound to the PM. The drift velocity of PM functionalized to single-strand DNA, which is proportional to the DEP force, was measured at the frequency range from 0.5 MHz to 2 MHz. The drift velocity was calculated using a custom-made automated software using real time image processing technique. This technology for SNP genotyping has the potential to be used in the diagnosis and the identification of genetic variants associated with diseases.

show abstract

Protein Dielectrophoresis and The Link to Dielectric Properties

Cited by 20 publications

References 146 publications

Rapid and selective concentration of bacteria, viruses, and proteins using alternating current signal superimposition on two coplanar electrodes

Rapid and selective concentration of bacteria, viruses, and proteins using alternating current signal superimposition on two coplanar electrodes

An Approach to Ring Resonator Biosensing Assisted by Dielectrophoresis: Design, Simulation and Fabrication

Nucleotide Identification in DNA Using Dielectrophoresis Spectroscopy

Contact Info

Product

Resources

About