Mango phenolics increase the serum apolipoprotein A1/B ratio in rats fed high cholesterol and sodium cholate diets

Abstract

We propose that phenolics are the most bioactive components of mango pulp, acting as anti-atherogenic and hepatoprotective agents, with a mechanism of action tentatively based on changes to the main protein components of lipoproteins. © 2018 Society of Chemical Industry.

Fiber and phenolic compounds contribution to the hepatoprotective effects of mango diets in rats fed high cholesterol/sodium cholate

“…Furthermore, MP significantly stimulated CAT and SOD gene expression, as compared with MF and FDM. In previous results (Domínguez‐Avila et al, ), MP fraction was able to exert higher protection against nonalcoholic steatosis by altering the APOA1/APOB protein expression and reducing food intake. Polyphenols have been considered signaling molecules that mitigate oxidative changes due to activation of transcription factors associated with inflammatory processes such as nuclear factor‐kappa B, mitogen‐activated protein kinase (MAPK), and nuclear factor‐erythroid derived 2 (Nrf2), making them key components to prevent/ameliorate noncommunicable diseases.…”

“…Ripe mango cv. Ataulfo pulp was obtained, frozen (−80°C, 24 hr) and freeze‐dried (−50°C, 0.03 mbar, 72 hR); a subsample of freeze‐dried mango pulp (FDM) was further treated to isolate mango fiber (MF) and mango phenolics (MPs) as previously described (Domínguez‐Avila et al, ). Food‐grade ingredients were used to prepare experimental diets were either from Bio‐Serv (Flemington, NJ, USA) or purchased in a local market; ACS analytical‐ or GC‐grade solvents, Tri‐Reagent and Supelco® 37‐fatty acid methyl ester mix were purchased from Sigma‐Aldrich (St. Louis, MO, USA).…”

“…The Tri‐Reagent was used to extract hepatic RNA as previously reported by Domínguez‐Avila et al (). Individual RNA samples were treated with RNAse‐free DNAse and reverse transcribed as indicated by the manufacturer (Promega).…”

“…Mango ( Mangifera indica L.) cv. “Ataulfo” contains dietary fiber and phenolic compounds with proven benefits in controlling plasma lipids in healthy humans (Robles‐Sanchez et al, ) and HCC‐fed rats (Domínguez‐Avila et al, ). Our group previously found that different fractions and doses of mango cv.…”

“…Our group previously found that different fractions and doses of mango cv. Ataulfo controlled plasma lipids in HCC‐fed rats (Domínguez‐Avila et al, ). Consumption of 50 g kg −1 of FDM showed a lesser hepatoprotective effect than MF (60 g kg −1 , e.g., attenuating hepatic enzyme activity), whereas the most antiatherogenic and anti‐NASH effects were exerted by the MP fraction (1 g kg −1 ), increasingserum APOA1/APOB protein ratio.…”