Malvidin Protects against and Repairs Peptic Ulcers in Mice by Alleviating Oxidative Stress and Inflammation

Abstract

Peptic ulcer episodes cause damage to the stomach and intestine, with inflammatory cell infiltration and oxidative stress as the main players. In this study, we investigated the potential of anthocyanidin malvidin for preventive and curative peptic ulcer treatment. The anthocyanidin effects were examined in gastric ulcer mouse models induced by ethanol, non-steroidal anti-inflammatory drugs (NSAIDs), ischemia-reperfusion (IR), acetic acid and duodenal ulcer induced by polypharmacy. Expression levels of oxidative and inflammatory genes were measured to investigate the mechanism of anthocyanin activity. At a dose of 5 mg·kg−1, Malvidin prevented gastric ulcer induction by ethanol, NSAID and repaired the tissue after 6 days of IR. Moreover, the anthocyanidin accelerated the healing of acetic acid-induced ulcer, increased the gene expression of EGF and COX-1, and downregulated MMP-9. Anthocyanin treatment mitigated the effect of polypharmacy on inflammation and oxidative stress observed in the intestine. Additionally, the compound downregulated cytokine expression and TLR4 and upregulated HMOX-1 and IL-10, exhibiting protective activity in the mouse gut. Malvidin thus prevented gastric and duodenal ulcers due to prominent anti-inflammatory and antioxidative effects on the gastrointestinal tract that were related to gene expression modulation and an increase in endogenous defense mechanisms.

In silico-based screening for natural product’s structural analogs as new drugs candidate against leishmaniasis

“…A major bottleneck of drug discovery on leishmaniasis was aimed at the in-silico workflow proposed, which is that compounds must show activity in the acidic environment of the phagolysosome [60]; thus, Malvidin showed stable receptor-ligand interaction and favorable binding free energy at pH 2 in MDS analysis. Also, the Target Product Profile (TPP) proposed by the Drugs for Neglected Diseases initiative (DNDi), includes regard for the oral route of administration for new candidates [61], while the ADME profile of the compound shows its potential for oral route administration and high bioavailability [62][63][64]. Furthermore, Malvidin has shown the potential to be an antioxidant, anti-hypertensive, antiinflammatory, anti-obesity, anti-osteoarthritis, anti-proliferative, and anticancer drug candidate [65][66][67][68][69].…”

Gastroprotective and Antioxidative Effects of the Traditional Thai Polyherbal Formula Phy-Blica-D against Ethanol-Induced Gastric Ulcers in Rats

“…Ethanol-induced gastric ulcer models are commonly used to evaluate the pathogenesis of gastric ulceration and investigate the gastroprotective effects of various drugs and natural products [ 40 ]. Previous studies have suggested that ethanol ingestion leads to gastric ulcers, which are significantly associated with increased oxidative stress and increases in the levels of free radicals derived from oxygen, leading to a reduction in antioxidant enzyme activity [ 41 ].…”

Anthocyanins in Chronic Diseases: The Power of Purple

“…Black rice extract containing cyanidin 3-glucoside inhibited the biogenesis of virulence proteins in H. pylori and decreased apoptosis of H. pylori -infected cells [ 257 ]. Further, reduction of oxidative stress and inflammation may play a role in the reduction of peptic ulcers caused by ethanol and non-steroidal anti-inflammatory drugs and increased healing of acetic-acid-induced ulcers by malvidin [ 258 ]. In DSS-induced IBD in rats, cyanidin 3-glucoside and extracts of Queen Garnet plums at 8 mg/kg/day effectively reversed gastrointestinal symptoms [ 259 ] to a similar extent as sulphasalazine (~350 mg/kg/day) [ 260 ].…”