Make hay when the sun shines: The role of MADS-box genes in temperature-dependant seasonal flowering responses

Hemming, Megan N.; Trevaskis, Ben

doi:10.1016/j.plantsci.2010.12.001

Cited by 64 publications

(49 citation statements)

References 73 publications

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“…The MADS-box genes encode a large family of transcription factors belonging to the gene clade represented by the Arabidopsis thaliana SEPALLATA3 E function gene (Honma and Goto, 2001). Previous studies have described MADS-box genes as master regulators of flowering transition and development of flowers, fruits, and seeds (Ma et al, 2011;Hemming and Trevaskis, 2011). Genome-wide studies suggest that most MADS-box genes are expressed in a variety of organs and cell types at different developmental stages (Rijpkema et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Differentially expressed genes implicated in embryo abortion of mango identified by suppression subtractive hybridization

He¹,

Ma²,

Chen³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Embryo abortion in mango severely damages mango production worldwide. The mechanisms by which the mango embryos abort have long been an intriguing question. We used subtractive suppression hybridization to investigate the differentially expressed genes involved in this process. We generated 2 cDNA libraries from normal seed and aborted seed embryos of mango cultivar 'Jinhuang'. One thousand five hundred and seventy-two high-quality expressed sequence tags (ESTs) were obtained, with 1092 from the normal seed tester library and 480 from the aborted seed tester library. These ESTs were assembled into 783 unigenes, including 147 contigs and 636 singletons in contigs; 297 singletons in gene ontology (GO) indicated coverage of a broad range of GO categories. Seven candidate genes from different categories were selected for semi-quantitative PCR 3967 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (4): 3966-3974 (2012) Differentially expressed genes in mango embryo abortion analysis, and their possible functions in embryo abortion are discussed. These data provide new insight into the genetic regulation of embryo abortion in mango and may aid in further identification of novel genes and their functions.

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Section: Discussionmentioning

confidence: 99%

Differentially expressed genes implicated in embryo abortion of mango identified by suppression subtractive hybridization

He¹,

Ma²,

Chen³

et al. 2012

Genet. Mol. Res.

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“…In temperate cereals, VRN1 is up-regulated during vernalization, coinciding with a reduction in H3K27me3 and an increase in H3K4me3 Hemming & Trevaskis, 2011).…”

Section: Chromatin Modification In Vernalizationmentioning

confidence: 99%

“…DAM5 and DAM6 levels directly correlated with the time required for bud break so they have been proposed as quantitative repressors of bud dormancy release (Jiménez et al, 2010b). The down-regulation of these genes is supposed to be necessary to reset dormancy mechanism for the next year (Hemming & Trevaskis, 2011). These observations were further confirmed by Yamane et al (2011) in Prunus persica treated with cyanamide, but also in other woody plants as Japanese apricot (Prunus mume) (Yamane et al, 2008), poplar (Druart et al, 2007), raspberry (Mazzitelli et al, 2007) and leafy spurge (Horvath et al, 2008).…”

Section: In Perennial Plants Short Vegetative Phase (Svp)-like/agamoumentioning

confidence: 99%

Molecular aspects of dormancy in peach (Prunus persica [L.] Batsch.)

Leida¹

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En estos cuatros años en los que he estado trabajando, he conocido a mucha gente que me ha ayudado en distintas formas para la realización de esta tesis y por eso he pensado en agradecerlos.En primer lugar quería dar las gracias a mis directores por la dedicación que me han prestado. A Gerardo y Marisa por darme la oportunidad de trabajar en el IVIA y de desarrollar esta tesis. A Gabino por haber estado ahí en todo momento, para enseñar y contestar a mis preguntas, sin su apoyo no habría podido llegar hasta aquí. ABSTRACTDormancy is one of the most important adaptive mechanisms developed by perennial plants, in order to survive the low temperatures of autumn and winter in temperate climates. The study of the genes regulated during dormancy release is crucial to understand the process, with the final objective of the development of new varieties with a better adaptation to certain environments; and this is particularly important considering the increasing economical weight of fruit crops in low and medium chilling regions as the Mediterranean area. We focused on the molecular and physiological mechanisms underlying the maintenance and release of seasonal dormancy in peach. In order to achieve this we first used suppression subtractive hybridization (SSH) to identify genes expressed in dormant and dormancy-released buds in two cultivars with different chilling requirements, 'Zincal-5' and 'Springlady', and subsequently validated their differential expression utilizing a peach cDNA microarray platform containing transcripts enriched in flower buds. Additionally, we carried out a genome-wide search of peach genes related to dormancy release by hybridizing the previous cDNA microarray with mRNA samples from 10 cultivars showing different dormancy behaviour, followed by an expression correlation analysis.Among the most relevant genes identified in these two first works, we found the DORMANCY ASSOCIATED MADS-box genes DAM4, DAM5 and DAM6, described independently by other groups working in peach and other species. The central role of DAM genes in dormancy regulation has also been confirmed by additional functional approaches as the analysis of the non-dormant evg mutant, QTL analysis, and transgenic approaches.In our second work we focused on the molecular mechanisms of DAM6 down-regulation concomitant with dormancy release in flower buds. A ChIP analysis of DAM6 promoter and structural gene revealed chromatin modification events similar to those observed in vernalization of Arabidopsis and cereals. We showed that DAM6 is transcriptionally active in dormant buds collected in October, when a short chromatin region around its ATG was trimethylated in histone H3 at K4 (H3K4) and acetylated at the N-terminal tail of H3. Concomitantly with DAM6 repression, H3K4 became demethylated and H3 deacetylated. Later H3K27 was found trimethylated along a genomic region larger than 4kb, including promoter, coding sequence and intron. Due to their relevance in dormancy regulation, DAM genes could be used as expression markers to assess...

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“…Many genes involved in flower development act in concert with physiological signals to establish complex gene regulatory networks that regulate flowering and floral organ development. Many of these genes belong to the MADS-box gene family, which is a large transcription factor family with diverse roles, especially floral development (Hemming and Trevaskis, 2011). Also, MADS genes are great significant for studying the system evolution of plant groups because of their universality and highly conservatism in the plant kingdom (Purugganan et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Expression Analysis of a MADS-Box Gene (<i>GbMADS2</i>) from <i>Ginkgo biloba

WANG

CHENG

et al. 2015

Not Bot Hort Agrobot Cluj

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As a kind of transcription factors gene family, MADS-box genes play an important role in plant development processes. To find genes involved in the floral transition of Ginkgo biloba, a MADS-box gene, designated as GbMADS2, was cloned from G. biloba based on EST sequences by RT-PCR. Sequence analysis results showed that the cDNA sequence of GbMADS2 contained a 663 bp length ORF encoding 221 amino acids protein, which displayed typical structure of plant MADS-box protein including MADS, I, and K domains and C terminus. The sequence of GbMADS2 protein was highly homologous to those of MADS-box proteins from other plant species with the highest homologous to AGAMOUS (CyAG) from Cycas revoluta. The phylogenetic tree analysis revealed that GbMADS2 belonged to AGAMOUS clade genes. Real-time PCR analysis indicated that expression levels of GbMADS2 gene in female and male flower were significantly higher than those in root, stem, and leaves, and that GbMADS2 expression level increased along with time of flower development. The spatial and timecourse expression profile of GbMADS2 implied that GbMADS2 might be involved in development of reproductive organs. The isolation and expression analysis of GbMADS2 provided basis for further studying the molecular mechanism of flower development in G. biloba.

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Make hay when the sun shines: The role of MADS-box genes in temperature-dependant seasonal flowering responses

Cited by 64 publications

References 73 publications

Differentially expressed genes implicated in embryo abortion of mango identified by suppression subtractive hybridization

Differentially expressed genes implicated in embryo abortion of mango identified by suppression subtractive hybridization

Molecular aspects of dormancy in peach (Prunus persica [L.] Batsch.)

Molecular Cloning and Expression Analysis of a MADS-Box Gene (<i>GbMADS2</i>) from <i>Ginkgo biloba

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