Differentially expressed genes implicated in embryo abortion of mango identified by suppression subtractive hybridization

He, Jieqiang; Ma, Furong; Chen, Y.Y.; Shu, Han

doi:10.4238/2012.november.14.4

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…Fruit abscission cause by embryo abortion presents in a variety of plants, such as apple [35] and mango [36]. However, there are few studies on embryo abortion-induced sweet cherry fruit shedding.…”

Section: Discussionmentioning

confidence: 99%

“…It can be inferred that the abortion of an embryo may cause fruit shedding. Both mango [36] and citrus [39] have proved that embryo abortion can lead to fruit abscission. Additionally, our group also found that the pollination trees have low pollen vigor and pollen deformity under an electron microscope (Unpublished).…”

Section: Embryo Abortion Leads To Fruit Abscissionmentioning

confidence: 99%

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Comparative Proteomics Profiling Illuminates the Fruitlet Abscission Mechanism of Sweet Cherry as Induced by Embryo Abortion

Qiu

Zhuang

Yang

et al. 2020

IJMS

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Sweet cherry (Prunus avium L.) is a delicious nutrient-rich fruit widely cultivated in countries such as China, America, Chile, and Italy. However, the yield often drops severely due to the frequently-abnormal fruitlet abscission, and few studies on the metabolism during its ripening process at the proteomic level have been executed so far. To get a better understanding regarding the sweet cherry abscission mechanism, proteomic analysis between the abscising carpopodium and non-abscising carpopodium of sweet cherry was accomplished using a newly developed Liquid chromatography-mass spectrometry/mass spectrometry with Tandem Mass Tag (TMT-LC-MS/MS) methodology. The embryo viability experiments showed that the vigor of the abscission embryos was significantly lower than that of retention embryo. The activity of cell wall degrading enzymes in abscising carpopodium was significantly higher than that in non-abscising carpopodium. The anatomy results suggested that cells in the abscission zone were small and separated. In total, 6280 proteins were identified, among which 5681 were quantified. It has been observed that differentially accumulated proteins (DAPs) influenced several biological functions and various subcellular localizations. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that plenty of metabolic pathways were notably enriched, particularly those involved in phytohormone biosynthesis, cell wall metabolism, and cytoskeletal metabolism, including 1-aminocyclopropane-1-carboxylate oxidase proteins which promote ethylene synthesis, and proteins promoting cell wall degradation, such as endoglucanases, pectinase, and polygalacturonase. Differential expression of proteins concerning phytohormone biosynthesis might activate the shedding regulation signals. Up-regulation of several cell wall degradation-related proteins possibly regulated the shedding of plant organs. Variations of the phytohormone biosynthesis and cell wall degradation-related proteins were explored during the abscission process. Furthermore, changes in cytoskeleton-associated proteins might contribute to the abscission of carpopodium. The current work represented the first study using comparative proteomics between abscising carpopodium and non-abscising carpopodium. These results indicated that embryo abortion might lead to phytohormone synthesis disorder, which effected signal transduction pathways, and hereby controlled genes involved in cell wall degradation and then caused the abscission of fruitlet. Overall, our data may give an intrinsic explanation of the variations in metabolism during the abscission of carpopodium.

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Section: Discussionmentioning

confidence: 99%

Section: Embryo Abortion Leads To Fruit Abscissionmentioning

confidence: 99%

Comparative Proteomics Profiling Illuminates the Fruitlet Abscission Mechanism of Sweet Cherry as Induced by Embryo Abortion

Qiu

Zhuang

Yang

et al. 2020

IJMS

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“…Otros autores mencionan que en el aborto del embrión influyen, además de altas y bajas temperaturas, algunos tratamientos químicos con biorreguladores, aberraciones cromosómicas y factores genéticos como genes que controlan la meiosis (Lin et al, 1984;Vardy et al, 1989). Estudios más recientes en el contexto molecular, señalan que el nivel de expresión del gen MaMADS es muy bajo en embriones abortados comparado con los embriones normales, por lo que este gen debe tener un papel importante en el desarrollo del embrión para dar lugar a un fruto normal (He et al, 2012). El objetivo del presente trabajo fue determinar los factores críticos del clima que inciden en la producción de frutos partenocárpicos en el cultivar Ataulfo y en que parte del desarrollo de la inflorescencia y fruto está afectando.…”

Section: Introductionunclassified

Temperatura y desarrollo floral en la formación de frutos partenocárpicos en mango ‘Ataulfo’

Pérez-Barraza

Bravo

García

et al. 2019

Remexca

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Uno de los principales problemas en mango ‘Ataulfo’ es el denominado ‘mango niño’ para referir a la alta producción de frutos partenocárpicos. El objetivo fue determinar los factores del clima que inciden en la presencia de frutos partenocárpicos y en que parte del desarrollo de la inflorescencia y fruto están afectando. El estudio se realizó de 2017 a 2018 en un huerto comerciales de mango ‘Ataulfo’, con una incidencia de 80% de frutos partenocárpicos, se seleccionaron 10 árboles y en cada árbol se marcaron 50 inflorescencias, en cada flujo de floración presentado, las cuales se encontraban distribuidas alrededor del árbol y en la parte media de la copa. Las variables evaluadas fueron el desarrollo de la inflorescencia, porcentaje y tipo de frutos (partenocárpico y con semilla), desarrollo de embrión y temperatura. Se presentaron dos flujos de floración. El segundo flujo de floración se prolongó por un período ligeramente mayor a 70 días y la etapa de plena floración hasta el amarre de fruto coincidió con temperaturas extremas, mínimas de 15 °C en promedio y máximas por arriba de los 35. La mayor producción de frutos partenocárpicos se presentó en el segundo flujo de floración con 75%, mientras que, en el primero la producción de frutos con semilla superó a los partenocárpicos. En Nayarit, la presencia de frutos partenocárpicos está relacionada con temperaturas extremas (≤15 y ≥35 °C) durante el periodo de plena floración hasta amarre de fruto, causando aborto de embrión.

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Transcriptome analysis of the differences in gene expression between testis and ovary in green mud crab (Scylla paramamosain)

et al. 2014

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BackgroundThe green mud crab (Scylla paramamosain) is the most prevalent crustacean on the southeast coast of China. The molecular regulatory mechanism of sex determination and gonadal differentiation in this species has received considerable attention in recent years because of the huge differences—both biological and economic—between male and female crabs. In this study, next-generation sequencing technology was used to develop deep-coverage transcriptomic sequencing data for the testis and ovary of S. paramamosain.ResultsA total of 365,116 reads (testis 171,962, ovary 193,154) with an average sequence length of 285 bp were produced from testis and ovary cDNA libraries. After filtering out contaminating reads, the clean reads were assembled, producing a total of 21,791 isotigs and leaving 22,814 reads as singlets. Using the BLASTX program, 3,471 unique sequences (2,275 isotigs and 1,196 singletons) were annotated with known protein sequences from the NCBI non-redundant (Nr) protein sequence database. The Gene Ontology and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses allowed the 224 unique sequences that were annotated with enzyme code (EC) numbers to be mapped into 174 KEGG pathways. After comparing the ovary and testis libraries, 4,021 gonad-differentially, 10,522 ovary-specifically, and 19,013 testis-specifically expressed genes were identified. Moreover, 33 ovary-specific, 14 testis-specific, and 34 gonad-differential transcripts were confirmed by semi-quantitative PCR and quantitative real-time PCR. In addition, 8,610 putative simple sequence repeats (SSRs) and 23,879 potential single nucleotide polymorphisms (SNPs) were identified.ConclusionThis is the first large-scale RNA sequencing of S. paramamosain to be reported. We have identified many important functional genes and made a preliminary attempt to construct the regulatory network involved in the gonadal development of crustaceans. The annotated transcriptome data will provide fundamental support for future research into the reproduction biology of S. paramamosain. A large number of candidate SSRs and SNPs were detected, which could be used as genetic markers for population genetics and functional genomics in this species.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-585) contains supplementary material, which is available to authorized users.

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Differentially expressed genes implicated in embryo abortion of mango identified by suppression subtractive hybridization

Cited by 4 publications

References 15 publications

Comparative Proteomics Profiling Illuminates the Fruitlet Abscission Mechanism of Sweet Cherry as Induced by Embryo Abortion

Comparative Proteomics Profiling Illuminates the Fruitlet Abscission Mechanism of Sweet Cherry as Induced by Embryo Abortion

Temperatura y desarrollo floral en la formación de frutos partenocárpicos en mango ‘Ataulfo’

Transcriptome analysis of the differences in gene expression between testis and ovary in green mud crab (Scylla paramamosain)

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