Major-to-Ultratrace Elements in Bone-Marrow Fluid as Determined by ICP-AES and ICP-MS

Abstract

The major-to-ultratrace elements in human bone-marrow fluid were determined by ICP-AES (inductively coupled plasma atomic emission spectrometry), and ICP-MS (inductively coupled plasma mass spectrometry). The bone-marrow fluid sample was centrifuged prior to acid digestion to exclude the bone piece from bone marrow, and then digested with nitric acid. As a result, 20 elements could be determined over the concentration range from 1610 µg g -1 for Na to 0.00043 µg g -1 for W. It was found that Fe, Zn and Sb were enriched by ca. 264-, 7-and 15-fold, respectively, in bone-marrow fluid, compared to those in human blood serum. Alkali metals (K, Rb, Cs), except for Na, were also significantly enriched in bone-marrow fluid. Furthermore, the concentrations of various elements, such as Fe, P, Al, Zn, Cu, Se, Zr, Sn, Ag and W, were significantly higher than those in open seawater.

Applications of ICP‐MS in Human Biomonitoring Studies

“…Dilution with nitric acid, Triton-X and butanol [25] Al, Be, Cd, Co, Cr, Hg, Mn, Ni, Pb, V [45] determination of 37 elements by ICP-MS [8]. Other methods of sample preparation for blood are dilution with HNO 3 [9,10] or tetra methyl ammonium hydroxide (TMAH) [11].…”

The Applicability of Plasma‐Based Techniques to Biological Monitoring

“…A comparative study was performed by analyzing stainless steel and commercially pure Ti plates after a 12 months implantation period [148]. Twenty elements were determined by ICP-AES and ICP-MS in human bone-marrow fluid [149]. Fourteen elements in human bones were investigated by ICP-AES to establish a database of elements in Taiwanese bones [150].…”

Reversal of Osteoporotic Changes of Mineral Composition in Femurs of Diabetic Rats by Insulin

“…The femurs were dried for 48 h at 80°C until the weight did not change. Sample digestion was undertaken following the literature [23,24], and the method was improved. In this case, each sample was taken into a PTFE Teflon beaker, predigested respectively in a mixture of HNO 3 and H 2 O 2 (4:1mixed, 5 ml per femur) overnight at room temperature to decompound organic component.…”