Magnetic stimulation of the angiogenic potential of mesenchymal stromal cells in vascular tissue engineering

Abstract

The growing prevalence of vascular diseases worldwide has emphasized the need for novel tissueengineered options concerning the development of vascularized 3D constructs. This study reports, for the first time, the use of external magnetic fields to stimulate mesenchymal stromal cells (MSCs) to increase the production of vascular endothelial growth factor-A (VEGF-A). Polyvinylalcohol and gelatin-based scaffolds, containing iron oxide nanoparticles, were designed for optimal cell magnetic stimulation. While the application of static magnetic fields over 24 h did not impact on MSCs proliferation, viability and phenotypic identity, it significantly increased the production of VEGF-A and guided MSCs morphology and alignment. The ability to enhance MSCs angiogenic potential was demonstrated by the increase in the number of new vessels formed in the presence of MSCs conditioned media through in vitro and in vivo models. Ultimately, this study uncovers the potential to manipulate cellular processes through short-term magnetic stimulation.

Biological Impact of γ-Fe2O3 Magnetic Nanoparticles Obtained by Laser Target Evaporation: Focus on Magnetic Biosensor Applications

“…The depletion of IL-6 production by myofibroblasts in a constant magnetic field, together with the absence of impact on IL-8 secretion, were also demonstrated [ 56 ]. In other studies, the enhancing effect of the constant magnetic field on VEGF secretion by mesenchymal cells was shown [ 57 ].…”

Micro/Nanosystems for Magnetic Targeted Delivery of Bioagents

“…Although the magnetic scaffolds are proven to trigger osteogenic differentiation and therefore have been mostly involved in bone tissue engineering, they have also shown utility for the repair of other tissue types [137,[217][218][219][220][221], and in relation to specific uses other than the direct modulation of cellular processes (e.g., bioimaging, cell patterning, robotics, drug delivery) [215,222]. In particular, magnetic actuation of scaffolds can serve to localize and control drug delivery [223].…”

Osteogenic potential of a 3D printed silver nanoparticle-based electroactive scaffold for bone tissue engineering using human Wharton's jelly mesenchymal stem cells

“…Utilization of external stimuli together with stimuliresponsive scaffolds could solve this problem because many external stimuli, such as magnetic, 4 mechanical, 5 temperature, 6,7 and electrical stimulation, [7][8][9] are able to improve the bone tissue regeneration rate. Among them, electrical stimulation is an attractive option because it has been demonstrated that sending signals across cell membranes with À10 to À90 mV would encourage cells to travel to the site of injury, 10 and also boost cardiac, 11 nerve, 12,13 and bone tissue regeneration.…”