Magnetic nanogold microspheres-based lateral-flow immunodipstick for rapid detection of aflatoxin B2 in food

Tang, Dianping; Sauceda, Jimena C; Lin, Zhen; Ott, Sonja; Basova, Evgenia Yu; Goryacheva, Irina Yu.; Biselli, Scarlett; Lin, Jing; Nießner, Reinhard; Knopp, Dietmar

doi:10.1016/j.bios.2009.07.030

Cited by 209 publications

(110 citation statements)

References 25 publications

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“…The second method consists on the use of the polysaccharidic region located in the Fc region of the antibody and the use of aminated nanoparticles. To our knowledge, few contributions in this line can be found on literature with magnetic microbeads and still less with magnetic nanoparticles [25][26][27][28][29][30][31][32]. The biofunctionalization of magnetic nanoparticles suffers some limitations when compared to surfaces and microbeads, such as easy aggregation with small changes of pH and/or ionic strength, polymeric shell instability, batch to batch MNPs irreproducibility, or presence of surfactants.…”

Section: Introductionmentioning

confidence: 99%

Designing novel nano-immunoassays: antibody orientation versus sensitivity

Puertas¹,

Moros²,

Fernández-Pacheco³

et al. 2010

J. Phys. D: Appl. Phys.

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There is a growing interest in the use of magnetic nanoparticles (MNPs) for their application in quantitative and highly-sensitive biosensors. The use of them as labels of biological recognition events and their detection by means of some magnetic method constitutes a very promising strategy for quantitative high-sensitive lateral-flow assays.In the present article, we report the importance of nanoparticle functionalization for the improvement of sensitivity for a lateral flow immunoassay. More precisely, we have found that immobilization of IgG anti-hCG through its polysaccharide moieties on magnetic nanoparticles allows more successful recognition of the hCG hormone. Although we used the detection of hCG as a model in this work, the strategy of binding antibodies to MNPs through its sugar chains reported here is applicable to other antibodies. Its potential is huge as it will be very useful for the development of quantitative and high-sensitive lateral-flow assays for its use on human and veterinary, medicine, food and beverage manufacturing, pharmaceutical, medical biologics and personal care product production, environmental remediation, etc.

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Section: Introductionmentioning

confidence: 99%

Designing novel nano-immunoassays: antibody orientation versus sensitivity

Puertas¹,

Moros²,

Fernández-Pacheco³

et al. 2010

J. Phys. D: Appl. Phys.

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show abstract

“…By considering these advantages, Tang and co-workers [93] synthetised the magnetic nano-gold microspheres (MnGMs; with nano-Fe 2 O 3 particles as core and GNPs as shell) as the colored reagent and employed them for detecting aflatoxin B2 (AFB2) in food samples. The synthesis of the MnGMs comprised six steps: 1) the synthesis of the SDS-Fe 2 O 3 complex; 2) immobilizing chitosan on the surface of Fe 2 O 3 nanoparticles; 3) the synthesis of the chitosan/Fe 2 O 3 nanocomposites; 4) cross-linking glutaraldehyde with chitosan, 5) assembling GNPs on the surface of the formed nanocomposites, 6) obtaining MnGMs by magnetic separation.…”

Section: Applications Of New Nanoparticle Labelsmentioning

confidence: 99%

Recent Advances in Nanoparticles-based Lateral Flow Biosensors

Gao¹,

Xu²,

Zhou³

et al. 2014

Am. J. Biomed. Sci.

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Lateral flow biosensors (LFBs) provide advantages in low cost, simplicity, rapidity, stability and portability, thus making LFBs popular in biomedical, agriculture, food and environmental sciences. This article reviews recent advances of LFBs for bioassays, including the investigation of the improvements achieved by signal-amplification strategies, the application of new nanoparticle labels, novel quantitative system and simultaneous detection. We summarized the outstanding performances of LFBs such as high detection sensitivity, specificity, reproducibility and reliability.

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“…[1][2][3][4][5][6][7][8][9] Among them, paper-based POC devices are a special category due to the advantages of being simple, rapid, on-site, and cost-effective; they have been widely used in home health care and medical testing, [10][11][12] even environmental monitoring. [13][14][15] The lateral flow immunochromatographic assay, also called lateral flow test, is one of the simplest and most popular formats of paper-based POC devices and can be used to detect specific substances, including small molecules, [15][16][17][18] large proteins, [19][20][21] and even whole pathogens, 10 in a sample by using an immunological reaction. However, most of these tests are based on qualitative colorimetry, which is not sufficient in many cases.…”

Section: Introductionmentioning

confidence: 99%

Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection

Zhu¹,

Sarwar²,

Yue³

et al. 2017

IJN

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Non-glucose biomarker-DNA oxidative damage biomarker 8-hydroxy-2′-deoxyguanosine (8-OHdG) has been successfully detected using a smartphone-enabled glucose meter. Through a series of immune reactions and enzymatic reactions on a solid lateral flow platform, 8-OHdG concentration has been converted to a relative amount of glucose, and therefore can be detected by conventional glucose meter directly. The device was able to detect 8-OHdG concentrations in phosphate buffer saline as low as 1.73 ng mL −1 with a dynamic range of 1–200 ng mL −1 . Considering the inherent advantages of the personal glucose meter, the demonstration of this device, therefore, should provide new opportunities for the monitoring of a wide range of biomarkers and various target analytes in connection with different molecular recognition events.

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Magnetic nanogold microspheres-based lateral-flow immunodipstick for rapid detection of aflatoxin B2 in food

Cited by 209 publications

References 25 publications

Designing novel nano-immunoassays: antibody orientation versus sensitivity

Designing novel nano-immunoassays: antibody orientation versus sensitivity

Recent Advances in Nanoparticles-based Lateral Flow Biosensors

Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection

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