Biosensing of DNA oxidative damage: a model of using glucose meter for non-glucose biomarker detection

Abstract

Non-glucose biomarker-DNA oxidative damage biomarker 8-hydroxy-2′-deoxyguanosine (8-OHdG) has been successfully detected using a smartphone-enabled glucose meter. Through a series of immune reactions and enzymatic reactions on a solid lateral flow platform, 8-OHdG concentration has been converted to a relative amount of glucose, and therefore can be detected by conventional glucose meter directly. The device was able to detect 8-OHdG concentrations in phosphate buffer saline as low as 1.73 ng mL −1 with a dynamic range of 1–200 ng mL −1 . Considering the inherent advantages of the personal glucose meter, the demonstration of this device, therefore, should provide new opportunities for the monitoring of a wide range of biomarkers and various target analytes in connection with different molecular recognition events.

Paper-Based Fluidic Sensing Platforms for β-Adrenergic Agonist Residue Point-of-Care Testing

“…15 IrO 2 @ PDA NPs: dopamine-modified iridium oxide nanoparticles. 16 PTAN: poly tannic acid nanospheres. 17 CuS@ Au: CuS nanospheres and Au nanoparticles.…”

“…Paper has become a simple and reliable platform for analytical instruments, and one of the most well-known applications is the lateral flow immunoassay (LFA). Due to its unique advantages, including rapidity, portability, and simplicity, LFAs have been widely used as tests for clinical diagnosis [ 15 , 16 ], infectious diseases [ 17 ], and the detection of chemical contaminants [ 18 ]. LFAs combine immune-specific recognition and sensitive nano-signal characteristics, creating an excellent tool for use in point-of-care tests (POCT) for food safety.…”

Antioxidant Effects of Vitamins C, E and Provitamin A Compounds as Monitored by Use of Biochemical Oxidative Indicators Linked to Atherosclerosis and Carcinogenesis

“…Among various types of DNA damage that occur due to Reactive oxygen species (ROS) and increase cancer risks, the most studied base damage is 8-oxoguanine (8-oxoG). Generation of 8-oxoG in DNA is a mutagenic and potentially carcinogenic event since the oxidized base has altered hydrogen bonding or "code specificity", preferentially forming base pairs with adenine rather than cytosine [23]. In addition, the deoxy-nucleoside derivative 8-OH-dG 8-hydroxy-2'deoxyguanosine has been used as useful marker of oxidative DNA damage [24].…”

Paper-Based Analytical Methods for Smartphone Sensing with Functional Nanoparticles: Bridges from Smart Surfaces to Global Health

“…In the past decade, paper-based colorimetric smartphone sensing platforms have been used to detect 8-hydroxy-2′-deoxyguanosine (8-OHdG), aflatoxin B1 (AFB1), alkaline phosphatase (ALP) activity, C-reactive protein (CRP), CA125, Cd­(II) and clenbuterol (CL), cocaine, Streptococcus pneumonia , , Escherichia coli , ,, ferritin, glucose, H 2 O 2 , Hg­(II), − immunoglobulin G (IgG), influenza A, melamine, Mycobacterium tuberculosis complex (MTBC) and tuberculosis (TB), − okadaic acid (OA) and saxitoxin (STX), plasmodium lactate dehydrogenase (pLDH), porcine epidemic diarrhea virus (PEDV), prostate-specific antigen (PSA), quinolones (QNs), and tetracyclines (TCs) . The most used real-life samples for these analytes are milk, ,,,,,, water, ,,− blood, , serum, ,,, and urine , samples.…”