Magnetic-fluorescent-targeting multifunctional aptasensorfor highly sensitive and one-step rapid detection of ochratoxin A

Wang, Chengquan; Qian, Jing; Wang, Kan; Wang, Kun; Liu, Qian; Dong, Xiaopeng; Wang, Chengke; Huang, Xingyi

doi:10.1016/j.bios.2015.02.008

Cited by 98 publications

(36 citation statements)

References 52 publications

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“…Various methods have been reported for laboratory assessment of ochratoxin A in food samples, including high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), gas chromatography-mass spectrometry (GC-MS), thin layer chromatography (TLC), enzyme-linked immunosorbent assay (ELISA), fluorimetry (FL), immunoassay based on fluorescence polarization (FPIA), and immunosensor based on surface plasmon resonance (SPR) 13 . One of the most common approaches among these mentioned methods for detection of OTA is the HPLC with fluorescence detector (HPLC/FLD) in which OTA must be extracted by the immunoaffinity column (IAC) 14 .…”

Section: Comparison Of Analytical Methods Used For Detection Of Ochramentioning

confidence: 99%

Different Applications of Magnetic Nanoparticles in the Rapid Monitoring of Ochratoxin A

Sargazi¹,

Majd²

2017

Orient. J. Chem

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Toxic effects of mycotoxins are still an unresolved subject for health of humans and animals. Therefore, several methods has been created to determine and eliminate mycotoxins from foodstuffs, and also are developing. For instance, utilization of magnetic nanoparticles (MNPs) when are combined with different analytical tools is one of the developing methods which could be useful for extraction of mycotoxins. As in some methods, they were employed as newfound adsorbents in magnetic solid phase extraction approaches. In some other methods, MNPs were used for immobilization of antibodies to enhance the efficiency of the analytical tools such as square wave voltammetry. Or in another method, amine-functionalized MNPs were reacted with ochratoxin A (OTA) to enhance performance of chemiluminescence immunoassay. Results of all methods were showed that utilizing of MNPs could reduce cost, easy preparation, and increase the stability, speed, sensitivity, precision and reproducibility. So, these new methods have elicited great hopes to be suitable alternative for older methods.

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Section: Comparison Of Analytical Methods Used For Detection Of Ochramentioning

confidence: 99%

Different Applications of Magnetic Nanoparticles in the Rapid Monitoring of Ochratoxin A

Sargazi¹,

Majd²

2017

Orient. J. Chem

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“…Ochratoxin A (OTA), a prevalent toxic mycotoxins, is generated by different specious of fungi, such as Penicillium and Aspergillus [1,2]. OTA can occur in a broad range of food matrices, such as grape juice, cereals, wine, barely, milk, and meat products [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…Generally, these detection procedures are laborious, expensive, and need skilled personnel and sophisticated instruments [1,2,6,8].…”

Section: Introductionmentioning

confidence: 99%

A fluorescent aptasensor based on a DNA pyramid nanostructure for ultrasensitive detection of ochratoxin A

et al. 2016

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Analytical techniques for detection of ochratoxin A (OTA) in food products and blood serum are of great significance. In this study, a fluorescent aptasensor was developed for sensitive and specific detection of OTA, based on a DNA pyramid nanostructure (DPN) and PicoGreen (PG) dye. The designed aptasensor inherits characteristics of DPN, such as high stability and capacity for PG loading. PG, as a fluorescent dye, could bind to double-stranded DNA (dsDNA). In the absence of OTA, the pyramid structure of DPN remains intact, leading to a very strong fluorescence emission. Because of higher affinity of aptamer for its target relative to its complementary strand, upon addition of target, the pyramid structure of DPN is disassembled, leading to a weak fluorescence emission. The presented aptasensor showed high specificity toward OTA with a limit of detection (LOD) as low as 0.135 nM. Besides, the designed sensing strategy was successfully utilized to recognize OTA in serum and grape juice with LODs of 0.184 and 0.149 nM, respectively.

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“…1 In great variety of foods, some species of Aspergillus fungi produce a carcinogenic metabolite called ochratoxin A (OTA). 2 Ochratoxin A is a stable molecule that resists degradation in acidic conditions, food processing and also blood serum with 35 days half-life.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4] Several methods for laboratory analysis and detection of ochratoxin A in food samples such as thin layer chromatography (TLC), high-performance liquid chromatography (HPLC), gas chromatographymass spectrometry (GC-MS), fluorescence polarization immunoassays, capillary electrophoresis (CE), enzymelinked immunosorbent assay (ELISA) and fluorescent and surface plasmon resonance immunosensors have been reported. 1,2,5 The most frequently used method for the determination of OTA in grains and many other foodstuffs is extraction and clean up via immunoaffinity column (IAC) before employing the HPLC. [6][7][8] In all validated methods for the detection of OTA based on IAC, solvents were utilized for extraction of OTA from real samples.…”

Section: Introductionmentioning

confidence: 99%

A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A

Sargazi¹,

Aliabadi²,

Rahdari³

et al. 2016

J. Braz. Chem. Soc.

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We report on the synthesis of dopamine loaded magnetic nanoparticles (MNPs) for new, simple, fast and repeatable extraction of ochratoxin A from different solvents and milk without utilizing immunoaffinity columns and even high-tech devices. To this end, Fe 3 O 4 nanoparticles (NPs) were synthesized using thermal decomposition reaction and dopamine (DPA) was then conjugated with Fe 3 O 4 nanoparticles (NPs) to form Fe 3 O 4 -DPA NPs. Dynamic light scattering, field emission scanning electron microscopy and transmission electron microscopy revealed an average size of about 15 nm for Fe 3 O 4 -DPA NPs. Moreover, zeta potential measurement and vibrating sample magnetometer confirmed positively charged (16.8 mV) and superparamagnetic behavior of MNPs, which are effective factors for a good adsorbent in the extraction. Various solvents and different effective parameters were measured until acetonitrile:methanol was selected as the best extraction solvent. In addition, based on the pH-partition theory, with changes in pH, we were able to increase and enhance the extraction rate to 94%. Moreover, the ability of Fe 3 O 4 -DPA NPs in solid phase extraction of ochratoxin A from spiked milk was evaluated. The recovery rate for extraction of OTA from milk was 68%.

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Magnetic-fluorescent-targeting multifunctional aptasensorfor highly sensitive and one-step rapid detection of ochratoxin A

Cited by 98 publications

References 52 publications

Different Applications of Magnetic Nanoparticles in the Rapid Monitoring of Ochratoxin A

Different Applications of Magnetic Nanoparticles in the Rapid Monitoring of Ochratoxin A

A fluorescent aptasensor based on a DNA pyramid nanostructure for ultrasensitive detection of ochratoxin A

A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A

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