A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A

Sargazi, Azam; Aliabadi, Amin; Rahdari, Ali; Allahdini-Hesaroiyeh, Samin; Nejati-Yazdinejad, Massoud; Majd, Mostafa Heidari

doi:10.21577/0103-5053.20160245

Cited by 5 publications

(6 citation statements)

References 29 publications

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“…Because, according to pH-partition theory, acids are un-ionized in acidic solutions and so the lowest amount of absorption occurs in the pH range from 1.0 to 5.0 22 . Since, this new method is inexpensive, reproducible and accurate, could be a good candidate for the determination of OTA in real samples in comparison with older methods.…”

Section: Ochratoxin a Determination By An Electrochemical Methods Usinmentioning

confidence: 99%

“…In a similar work which was done by Sargazi et al, dopamine loaded MNPs were synthesized to be utilized as a nanosized functional adsorbent in extraction of OTA 22 . Because in proper condition, the terminal amino group of dopamine has the positively charged (-NH 3 + ) 18 After examination of different parameters which affect the efficiency of new adsorbents, the selectivity of magnetic sorbents for OTA in real samples such as cereal and rice were confirmed.…”

Section: Fig 2: Schematically Demonstrates Fabrication Of the Mns-apmentioning

confidence: 99%

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Different Applications of Magnetic Nanoparticles in the Rapid Monitoring of Ochratoxin A

Sargazi¹,

Majd²

2017

Orient. J. Chem

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Toxic effects of mycotoxins are still an unresolved subject for health of humans and animals. Therefore, several methods has been created to determine and eliminate mycotoxins from foodstuffs, and also are developing. For instance, utilization of magnetic nanoparticles (MNPs) when are combined with different analytical tools is one of the developing methods which could be useful for extraction of mycotoxins. As in some methods, they were employed as newfound adsorbents in magnetic solid phase extraction approaches. In some other methods, MNPs were used for immobilization of antibodies to enhance the efficiency of the analytical tools such as square wave voltammetry. Or in another method, amine-functionalized MNPs were reacted with ochratoxin A (OTA) to enhance performance of chemiluminescence immunoassay. Results of all methods were showed that utilizing of MNPs could reduce cost, easy preparation, and increase the stability, speed, sensitivity, precision and reproducibility. So, these new methods have elicited great hopes to be suitable alternative for older methods.

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Section: Ochratoxin a Determination By An Electrochemical Methods Usinmentioning

confidence: 99%

Section: Fig 2: Schematically Demonstrates Fabrication Of the Mns-apmentioning

confidence: 99%

Different Applications of Magnetic Nanoparticles in the Rapid Monitoring of Ochratoxin A

Sargazi¹,

Majd²

2017

Orient. J. Chem

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“…As a result, these flavonoids exhibited various inhibition on alpha-amylase and DPPH free radical scavenging activities. Apigenin, kaempferol and (2), isoquercitrin (3), taxifolin (4), luteolin (5), quercetin (6), apigenin (7), naringenin (8), kaempferol (9) and isorhamnetin (10)); (b) lotus leaf extract. isorhamnetin showed relatively higher inhibition on alphaamylase, which exhibited higher binding degrees as well.…”

Section: Alpha-amylase Inhibitors Screening From Flavonoidsmentioning

confidence: 99%

“…5 In recent years, many methods and technologies were used in the screening and analysis of active compounds such as filtration, solid phase extraction and hyphenated instruments. [6][7][8][9] Centrifugal ultrafiltration is a kind of membrane separation technique, which could retain high molecular weight compounds on the membrane, and low molecular weight compounds could pass through the membrane during centrifugation. 10 Centrifugal ultrafiltration could be used for the screening of bioactive constituents for target biomolecules from complexes.…”

Section: Introductionmentioning

confidence: 99%

Screening and Binding Analysis of Flavonoids with Alpha-Amylase Inhibitory Activity from Lotus Leaf

Liao¹,

Chen²,

Liu³

et al. 2017

J. Braz. Chem. Soc.

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Lotus leaf is gaining growing popularity due to its various benefits and widely usage. In this paper, ten flavonoids in lotus leaf extract were analyzed by high performance liquid chromatography (HPLC). Centrifugal ultrafiltration combined liquid chromatography was used to screen alphaamylase inhibitors from ten flavonoids mixture and the binding degrees ranged from 2.34 to 94.1%. The alpha-amylase inhibition and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) antioxidant activity of ten flavonoids were verified as well. Apigenin, kaempferol and isorhamnetin with relatively higher binding degrees showed higher inhibition on alpha-amylase as well. The interactions between these flavonoids and alpha-amylase were investigated by spectroscopic method. As a result, the fluorescence quenching could be considered as static quenching because the quenching rate constant (K q ) values were higher than 2.0 × 10 10 L mol -1 s -1. The binding constants (log 10 K a ) of ten flavonoids were in the range of 5.971 to 7.417 L mol -1 . The hydrogenation of C 2 =C 3 double bond of apigenin and quercetin decreased the affinity for alpha-amylase (1.92-and 2.82-fold). The hydroxylation on 3 and 3' position decreased the affinity for alpha-amylase. Moreover, the glycosylation with different sugar moiety improved in varying degrees of affinity. The hydrogen bond force might be important in the binding between alpha-amylase and flavonoids.

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“…This biocompatible polymer could localize in the tumours due to enhanced permeability and retention (EPR) effect, therefore, achieving slow clearance, extended-interval dosing and less toxicity of the carried drugs [6]. On the other hand, polymeric NPs can be activated for further functionalization with targeting and/or therapy agents [4,7,8].…”

Section: Introductionmentioning

confidence: 99%

Hyaluronic acid/polyethylene glycol nanoparticles for controlled delivery of mitoxantrone

Sargazi

Kamali

Shiri

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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Hyaluronic acid (HA) has inherent ability to target the CD44 receptors and internalize into tumour cells via receptor-mediated endocytosis. Therefore, conjugation of this natural linear polysaccharide to polymeric NPs or micelles, as one of the most promising approaches, could be useful for future clinical applications such as drug delivery. Accordingly, we report on the synthesis of mitoxantrone (MTX)-conjugated polymeric nanoparticles (NPs) composed of polyethylene glycol-HA (PEG-HA) for MTX delivery toward special tumour cells. To determine the size of the polymeric NPs, field emission scanning electron microscopy (FESEM) and particle size analyzer system Zetasizer_nanoZS were employed. The in vitro cytotoxicity analysis of MTX-loaded HA-PEG NPs and free MTX against two cell lines with different levels of CD44 expression (MDA-MB-231 (very high) and MCF-7 (low) was conducted by MTT assay. Also, computational molecular docking was employed to study in detail the active site residues and the critical interactions between HA-EDA-PEG-EDA-MTX NPs and CD44 receptor. The particle size analysis and electron microscopy showed the average size of polymeric NPs less than 350 nm. FT-IR spectrophotometry analysis and also NMR confirmed the conjugation of HA and MTX onto the PEG. Cytotoxicity assay revealed that the engineered polymeric NPs were able to specifically bind to and significantly inhibit the CD44 receptor-positive MDA-MB-231 cells, but not the CD44-negative MCF-7 cells. Furthermore, analysis of the binding modes revealed that for the best-docked pose nearly 10 conventional hydrogen bond can occur between the MTX-EDA-PEG-EDA-HA NPs and amino acids of CD44 receptor. Based on these findings, we suggest the HA-PEG-MTX NPs as an effective functional-targeted nanomedicine toward therapy of CD44-positive cancers.

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A Simple and Fast Method for Magnetic Solid Phase Extraction of Ochratoxin A

Cited by 5 publications

References 29 publications

Different Applications of Magnetic Nanoparticles in the Rapid Monitoring of Ochratoxin A

Different Applications of Magnetic Nanoparticles in the Rapid Monitoring of Ochratoxin A

Screening and Binding Analysis of Flavonoids with Alpha-Amylase Inhibitory Activity from Lotus Leaf

Hyaluronic acid/polyethylene glycol nanoparticles for controlled delivery of mitoxantrone

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