Different Applications of Magnetic Nanoparticles in the Rapid Monitoring of Ochratoxin A

Sargazi, Azam; Majd, Mostafa Heidari

doi:10.13005/ojc/330141

Cited by 6 publications

(4 citation statements)

References 26 publications

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“…Notably, no corresponding reduction peak is observed in the reverse sweep of the cyclic voltammogram, which indicates that the electrode reaction of OTA is an irreversible process, as detailed in Scheme . This electrochemical behavior is in agreement with the results of a previous study investigating OTA oxidation .…”

Section: Resultsmentioning

confidence: 99%

Adsorptive Voltammetry for the Determination of Ochratoxin A Using Enrichment Effect by Cationic Surfactants

et al. 2018

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A rapid and simple electrochemical method was utilized for the determination of the presence of classic fungal metabolite ochratoxin A (OTA) based on differential pulse adsorptive voltammetry, using the carbon nanotube paste (CNTP) electrode. OTA showed a clear oxidation peak, and the CNTP electrode exhibited a more sensitive response to OTA oxidation than the glassy carbon, the plastic formed carbon, and the carbon paste electrodes. The addition of long‐chain cationic surfactants, such as benzethonium chloride, cetyltrimethylammonium bromide, and benzyldimethyltetradecylammonium chloride (zephiramine), enhanced the electrochemical response of OTA. Under optimized experimental conditions, the calibration plots are linear in the 5×10−6−5×10−5 M OTA concentration range, and the limit of detection is 1.1×10−6 M. In order to improve the selectivity for OTA determination, we attempted to combine this adsorptive stripping voltammetric technique with “sequestration electrochemistry,” based on the specific interaction occurring between bovine serum albumin (BSA) and OTA. The peak current of OTA oxidation decreased substantially as a result of OTA‐BSA binding. Furthermore, it was demonstrated that the application of this proposed method of hydrodynamic adsorptive voltammetry, using a micro‐droplet and a rotating disk electrode, improves the approach's sensitivity and reduces the time and sample volume required for the analysis.

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Section: Resultsmentioning

confidence: 99%

Adsorptive Voltammetry for the Determination of Ochratoxin A Using Enrichment Effect by Cationic Surfactants

et al. 2018

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“…As described in our previous work, the surface of Fe 3 O 4 was modified using the dopamine moiety of "DPN. " Dopamine, which acts as an anchoring agent, could replace the oleylamine on the surface of Fe 3 O 4 NPs [27] . The final size of Fe 3 O 4 -DPN was 13 (±0.4) nm [28] .…”

Section: Characterization Of Fe 3 O 4 -Dpn-ha-famentioning

confidence: 99%

Dual-targeting and specific delivery of tamoxifen to cancer cells by modified magnetic nanoparticles using hyaluronic acid and folic acid

Majd

2022

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Tamoxifen (TMX) which serves as the best clinical option for the treatment of breast cancer may trigger major dose-dependent side effects due to its poor solubility. Therefore, the use of lower TMX doses utilizing nano-enabled drug delivery systems offers multiple benefits to improving drug specified concentration, safety, and long-term release. In this study, we synthesized targeted magnetic nanoparticles (MNPs) containing folic acid (FA) and hyaluronic acid (HA) to improve drug delivery of TMX. After investigations utilizing Fourier-transform infrared spectroscopy and field emission scanning electron microscope, we found that the surface of MNPs was well modified with targeting agents, and the size of the Fe3O4-DPN-HA-FA NPs was determined at ∼153 (±3.3) nm. Furthermore, the release of 81% TMX after 120 h indicated that there was a controlled pattern of drug release from the modified MNPs. Besides that, the MTT assay revealed that the viability of MDA-MB-231 cell lines after 48 h and 72 h of treatment is dependent on the time and concentration of Fe3O4-DPN-HA-FA-TMX NPs. Finally, real-time polymerase chain reaction demonstrated that Fe3O4-DPN-HA-FA-TMX NPs could upregulate the expression of Bak1 genes and downregulated the expression of Bclx genes during 24 h treatment. All data confirmed that the presence of HA and FA on the surface of nanocarriers and the active targeting employing the nanocarriers can be a useful step to obliterate the breast cancer cells.

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“…There are many publications on IFA developed using MNPs (Godjevargova et al 2019;Ivanov et al 2019;Qin et al 2019). Usually the MNPs are used for extraction of OTA, and its detection is performed with different analytical tools (Sargazi and Majd 2017). In some other variants, MNPs are used for immobilization of antibodies to enhance the efficiency of the analytical tools such as square wave voltammetry, ELISA, and amperometric biosensor (Kim and Lim 2015;Zhang et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Magnetic Nanoparticle-Based Fluorescence Immunoassay for Determination of Ochratoxin A in Milk

et al. 2020

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Ochratoxins are possible human carcinogens. The aim of this study is to develop a rapid and sensitive competitive immunofluorescent analysis for determination of ochratoxin A (OTA) on the base of immobilized polyclonal antibody against ochratoxin and immobilized F(ab′) 2 fragment on magnetic nanoparticles (MNPs). F(ab′) 2 fragment of anti-OTA antibody was obtained by pepsin hydrolysis of polyclonal antibody against OTA. The competitive fluorescent conjugate OTA-OVA-FITC (OTA coupled to ovalbumin (OVA) and then conjugated to fluorescein isothiocyanate (FITC)) was prepared and purified by size-exclusion chromatography. Competitive immunoassay was performed by using obtained immobilized antibody or F(ab′) 2 fragment on magnetic nanoparticles and the conjugate OTA-OVA-FITC. The analytical characteristics of the analysis with immobilized polyclonal antibody and F(ab′) 2 fragment were compared. The linear measuring range of OTA in milk, obtained with immobilized whole antibody, was from 0.1 to 2.5 ng/mL OTA and with immobilized F(ab′) 2 fragment from 0.1 to 7.5 ng/mL OTA. The detection limit of immunoassay with immobilized whole antibody was 0.1 ng/mL OTA and with immobilized F(ab′) 2 fragment was 0.08 ng/mL OTA. Milk samples were spiked with OTA at different levels. The recovery rates when using immobilized F(ab′) 2 fragment were between 99.4 and 118.0%, and the relative standard deviations (RSDs) were 6.5-7.7%. The results indicate that this fluorescence immunoassay with MNPs was accurate and has good reproducibility.

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Different Applications of Magnetic Nanoparticles in the Rapid Monitoring of Ochratoxin A

Cited by 6 publications

References 26 publications

Adsorptive Voltammetry for the Determination of Ochratoxin A Using Enrichment Effect by Cationic Surfactants

Adsorptive Voltammetry for the Determination of Ochratoxin A Using Enrichment Effect by Cationic Surfactants

Dual-targeting and specific delivery of tamoxifen to cancer cells by modified magnetic nanoparticles using hyaluronic acid and folic acid

Magnetic Nanoparticle-Based Fluorescence Immunoassay for Determination of Ochratoxin A in Milk

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