Magnetic coupling of creatine/phosphocreatine protons in rat skeletal muscle, as studied by1H-magnetization transfer MRS

Kruiskamp, Marijn J.; Graaf, Robin A. de; Vliet, Gerard van; Nicolay, Klaas

doi:10.1002/(sici)1522-2594(199910)42:4<665::aid-mrm7>3.0.co;2-9

Cited by 38 publications

(44 citation statements)

References 30 publications

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“…19 Whether or to what extent such a two-site exchange mechanism exists for Cr/PCr, remains uncertain. A different situation is observed in rat skeletal muscle, where the NMR detectable Cr/PCr concentration is 27 mM, 45 while 38 mM are found biochemically in tissue extracts. 55 This invisible Cr/ PCr amount is larger than needed to account for the MT effect or be bound to CK.…”

Section: In Vivo Applicationsmentioning

confidence: 85%

“…The echo time was 68, 136 or 272 ms. The magnetization transfer effect on Cr/PCr was subsequently confirmed in various studies in rat brain [17][18][19]44 and skeletal muscle, [45][46][47][48][49] and in human brain. [50][51][52] Various mechanisms may account for the observed MT effect:…”

Section: In Vivo Applicationsmentioning

confidence: 86%

“…Subsequently bulk water transfers magnetization via intermolecular cross relaxation to creatine. 11,12,62 The involvement of mobile bulk water becomes obvious from MT via low energy saturation of mobile bulk water, 45,48 cf. Fig.…”

Section: Intermolecular Cross Relaxation Most Likely Accountsmentioning

confidence: 99%

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Magnetization transfer MRS

Leibfritz¹,

Dreher²

2001

NMR in Biomedicine

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This review deals with magnetization transfer (MT) effects observed in in vivo NMR spectroscopy. The basic experimental methods of MT experiments, the underlying kinetic mechanisms as well as the evaluation of measured data by fits to two-or three-pool models are described. Experimental results of both 31 P and 1 H in vivo MRS are reviewed showing the potential of MT experiments to characterize kinetic equilibrium reactions. This includes reactions where all involved components are MR visible, as well as situations where one indirectly measures pools of bound spins which cannot directly be observed in vivo. In particular, MT effects are described which have been observed in in vivo 1 H NMR spectra measured on the animal or human brain or on skeletal muscle. Possible mechanisms for the strong MT effects observed for the signals of creatine/phosphocreatine, lactate, alcohol and other metabolites are discussed. It is also emphasized that MT effects caused by water suppression techniques may lead to systematic errors in the quantification of in vivo 1 H NMR spectra.

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Section: In Vivo Applicationsmentioning

confidence: 85%

Section: In Vivo Applicationsmentioning

confidence: 86%

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Magnetization transfer MRS

Leibfritz¹,

Dreher²

2001

NMR in Biomedicine

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“…[16][17][18] Agreement between calculations and measured data to within 1-3% has allowed quantitative parameterization of biophysical models of MT in tissues. Agar was the first model system to be studied in detail.…”

Section: Experimental Demonstrationsmentioning

confidence: 94%

“…Not surprisingly, the macromolecular absorption lineshape for tissues is not Gaussian as it is in agar, but is 'softer'. Reasonable fits can be obtained using a super-Lorentzian lineshape 17 as is done for liquid crystals. However, given the variety of macromolecular protons in tissue, it seems better not to use some a priori lineshape, but instead to let the data 'speak for itself' and to extract a parameterized absorption lineshape from the measured MT experiments.…”

Section: Experimental Demonstrationsmentioning

confidence: 99%

Magnetization transfer in MRI: a review

2001

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This review describes magnetization transfer (MT) contrast in magnetic resonance imaging. A qualitative description of how MT works is provided along with experimental evidence that leads to a quantitative model for MT in tissues. The implementation of MT saturation in imaging sequences and the interpretation of the MT-induced signal change in terms of exchange processes and direct effects are presented. Finally, highlights of clinical uses of MT are outlined and future directions for investigation proposed.

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