Proton MR spectroscopy of wild-type and creatine kinase deficient mouse skeletal muscle: Dipole-dipole coupling effects and post-mortem changes

Zandt, René in ‘t; Klomp, Dennis W. J.; Oerlemans, Frank; Wieringa, Bé; Hilbers, C. W.; Heerschap, Arend

doi:10.1002/(sici)1522-2594(200004)43:4<517::aid-mrm5>3.0.co;2-i

Cited by 42 publications

(8 citation statements)

References 22 publications

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“…The splittings due to these residual dipolar couplings appeared to be orientation‐dependent, and it was shown that the residual dipolar couplings are proportional to the angle θ between the muscle fibers and the main magnetic field B 0 according to

. 14 , 15 , 16 , 17 , 19 , 20 , 21 , 22 …”

Section: Discussionmentioning

confidence: 99%

“…In hydrogen‐1 ( 1 H) MR spectra, this leads to residual dipolar coupling splittings for the resonances of Cr, taurine, and lactate as a result of incomplete motional averaging of the orientation‐dependent (anisotropic) dipolar interactions. 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 For 2 H nuclei, next to the effects of dipolar couplings, we also have to consider the residual effects of the quadrupolar coupling when motion is not completely isotropic. Both from ab initio calculations and NMR experiments, the deuteron quadrupolar coupling constant (C Q ) for a deuterated water (HDO) molecule in a liquid environment has been determined to be ~250 kHz at body temperature.…”

Section: Introductionmentioning

confidence: 99%

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Residual quadrupolar couplings observed in 7 Tesla deuterium MR spectra of skeletal muscle

Gursan

Froeling

Hendriks

et al. 2021

Magnetic Resonance in Med

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. 14 , 15 , 16 , 17 , 19 , 20 , 21 , 22 …”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Residual quadrupolar couplings observed in 7 Tesla deuterium MR spectra of skeletal muscle

Gursan

Froeling

Hendriks

et al. 2021

Magnetic Resonance in Med

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“…All subjects were supinely positioned (feet first) with their right leg inside a transmit coil. To improve separation between IMCL and EMCL and retain residual dipolar couplings, care was taken to position the leg parallel to the Bo field [19,28]. This study was approved by the local institutional review board.…”

Section: Methodsmentioning

confidence: 99%

In vivo 1D and 2D correlation MR spectroscopy of the soleus muscle at 7T

Ramadan¹,

Ratai

Wald

et al. 2010

Journal of Magnetic Resonance

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Aim This study aims to (1) undertake and analyse 1D and 2D MR correlation spectroscopy from human soleus muscle in vivo at 7T, and (2) determine T1 and T2 relaxation time constants at 7T field strength due to their importance in sequence design and spectral quantitation. Method Six healthy, male volunteers were consented and scanned on a 7T whole-body scanner (Siemens AG, Erlangen, Germany). Experiments were undertaken using a 28 cm diameter detunable birdcage coil for signal excitation and an 8.5 cm diameter surface coil for signal reception. The relaxation time constants, T1 and T2 were recorded using a STEAM sequence, using the ‘progressive saturation’ method for the T1 and multiple echo times for T2. The 2D L-Correlated SpectroscopY (L-COSY) method was employed with 64 increments (0.4 ms increment size) and eight averages per scan, with a total time of 17 min. Results T1 and T2 values for the metabolites of interest were determined. The L-COSY spectra obtained from the soleus muscle provided information on lipid content and chemical structure not available, in vivo, at lower field strengths. All molecular fragments within multiple lipid compartments were chemically shifted by 0.20–0.26 ppm at this field strength. 1D and 2D L-COSY spectra were assigned and proton connectivities were confirmed with the 2D method. Conclusion In vivo 1D and 2D spectroscopic examination of muscle can be successfully recorded at 7T and is now available to assess lipid alterations as well as other metabolites present with disease. T1 and T2 values were also determined in soleus muscle of male healthy volunteers.

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“…In the same context, the TMA peak of acetylcarnitine (slightly upfield-shifted compared with TMA of free carnitine) has been found to be represented by a broad signal pattern with possibly reduced visibility at 1.5 and 3 T, 13 although it was reported to be fully visible at 7 T. 14 Furthermore, free Cr seems to be invisible while PCr remains detectable 15 under certain conditions. Whether this effect depends on external field strength, muscle or species type has so far remained open to debate 16,17 but may well be linked to multiple compartments of Cr/PCr that have been observed in other contexts.…”

Section: Mr Visibility Of Metabolitesmentioning

confidence: 99%

“…Furthermore, free Cr seems to be invisible while PCr remains detectable 15 under certain conditions. Whether this effect depends on external field strength, muscle or species type has so far remained open to debate 16,17 but may well be linked to multiple compartments of Cr/PCr that have been observed in other contexts. As a consequence of the limited visibility and orientation‐dependent resonance patterns, the use of the 3 ppm signal of total creatine (tCr) as standard for conversion to absolute concentration units is discouraged.…”

Section: Anatomical Functional and Metabolic Properties Of Skeletal Musclementioning

confidence: 99%

Proton magnetic resonance spectroscopy in skeletal muscle: Experts' consensus recommendations

et al. 2020

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H-MR spectroscopy of skeletal muscle provides insight into metabolism that is not available noninvasively by other methods. The recommendations given in this article are intended to guide those who have basic experience in general MRS to the special application of 1 H-MRS in skeletal muscle. The highly organized structure of skeletal muscle leads to effects that change spectral features far beyond simple peak heights, depending on the type and orientation of the muscle. Specific recommendations are given for the acquisition of three particular metabolites (intramyocellular lipids, carnosine and acetylcarnitine) and for preconditioning of experiments and instructions to study volunteers.

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Proton MR spectroscopy of wild-type and creatine kinase deficient mouse skeletal muscle: Dipole-dipole coupling effects and post-mortem changes

Cited by 42 publications

References 22 publications

Residual quadrupolar couplings observed in 7 Tesla deuterium MR spectra of skeletal muscle

Residual quadrupolar couplings observed in 7 Tesla deuterium MR spectra of skeletal muscle

In vivo 1D and 2D correlation MR spectroscopy of the soleus muscle at 7T

Proton magnetic resonance spectroscopy in skeletal muscle: Experts' consensus recommendations

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