Magnesium 2,4-divinylphaeoporphyrin a5 monomethyl ester, a protochlorophyll-like pigment present in some unicellular flagellates

Ricketts, T. R.

doi:10.1016/s0031-9422(00)85121-3

Cited by 48 publications

(25 citation statements)

References 12 publications

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“…There are several reports on the spectral properties of DVPchlide (3,5,7,12); however, only one report is available on the Pchlide specifically designated as MV-Pchlide (1). In this study, we found that MV-Pchlide could be purified selectively and effectively by choosing specific plant materials at certain ages.…”

Section: Resultsmentioning

confidence: 68%

Monovinyl and Divinyl Protochlorophyllide Pools in Etiolated Tissues of Higher Plants

Shioi¹,

Takamiya²

1992

Plant Physiol.

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The composition of chlorophyll-precursor pigments, particularly the contents of monovinyl (MV) and divinyl (DV) protochlorophyllides (Pchlides), in etiolated tissues of higher plants were determined by polyethylene-column HPLC (Y. Shioi, S. I. Beale [1987] Anal Biochem 162: 493-499), which enables the complete separation of these pigments. DV-Pchlide was ubiquitous in etiolated tissue of higher plants. From the analyses of 24 plant species belonging to 17 different families, it was shown that the concentration of DV-Pchlide was strongly dependent on the plant species and the age of the plants. The ratio of DV-Pchlide to MV-Pchlide in high DV-Pchlide plants such as cucumber and leaf mustard decreased sharply with increasing age. Levels of DV-Pchlide in Gramineae plants were considerably lower at all ages compared with those of other plants. Etiolated tissues of higher plants such as barley and corn were, therefore, good sources of MV-Pchlide. Absorption spectra of the purified MV-and DV-Pchlides in ether are presented and compared.atives have been accomplished mainly by fluorescence spectroscopy at low temperature (1, 10) or by TLC using polyethylene as a support (11). We previously developed a separation technique for the mixture of MV-and DV-pigment derivatives by HPLC using a polyethylene column and simple elution with aqueous acetone (6). Our experimental system allowed the rapid separation of these pigment mixtures with high resolution and sensitivity at the picomole level.We attempted to quantitate MV-and DV-Pchlides in etiolated tissues of higher plants by means of our HPLC system. The analytical data obtained from 24 plant species belonging to 17 different families and different ages showed the ubiquitous occurrence of DV-Pchlide in addition to MV-Pchlide in etiolated leaves of higher plants. Our results also give further information on changing levels of MV-and DVPchlides during dark growth. MATERIALS AND METHODS Plant MaterialsEtiolated tissues of higher plants accumulate the Chlprecursor pigments Pchlide and its esterified form, Pchl.2 Considerable experimental evidence indicates that these pigments are not chemically homogeneous; Pchlide consists of MV3 and DV derivatives (1) (Fig. 1), and Pchl comprises at least four species esterified with different alcohols (9). Tripathy and Rebeiz (10) reported that in higher plants, MV-and DV-Pchlides were formed from MV-and DV-protoporphyrin via MV-and DV-monocarboxylic biosynthetic routes, respectively. They also described four types of greening groups in higher plants on the basis of MV-or DV-monocarboxylic biosynthetic routes that predominate at night or in daylight (11). Thus, they proposed a parallel biosynthetic pathway for the formation of Chl precursors as opposed to the traditional single pathway (for review, see ref.2). However, little information is available concerning the changing levels of DV-and MV-Pchlide during dark growth, particularly in different plant species.Until recently, detection and quantification of both deriv-'

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Section: Resultsmentioning

confidence: 68%

Monovinyl and Divinyl Protochlorophyllide Pools in Etiolated Tissues of Higher Plants

Shioi¹,

Takamiya²

1992

Plant Physiol.

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“…Aside from the main pigments which are also characteristic of higher plants, within the prasinophyceae some class-specific pigments such as prasinoxanthin and Mg-2,4-divinyl-phaeoporphyrin-a 5 -monomethylester were observed in previous studies (Ricketts 1966, Foss et al 1984, Wilhelm et al 1986, Fawley & Lee 1990, Fawley 1992, or siphonaxanthin in some orders of the ulvophyceae and prasinophyceae (Kleinig 1969, Weber & Czygan 1972, Anderson 1983, Chu & Anderson 1985. In this study, none of these carotenoids was encountered in the chlorophyceae, supporting the classification of major lineages within the green algae on basis of molecularbiological and ultrastructural data.…”

Section: Chlorophyceaementioning

confidence: 89%

Patterns of major photosynthetic pigments in freshwater algae. 2. Dinophyta, Euglenophyta, Chlorophyceae and Charales

Schagerl

Pichler

Donabaum³

2003

Ann. Limnol. - Int. J. Lim.

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“…The last polar ChI c-like compound detected in the Prymnesiophyceae was MgDVP (Garrido et al 1995), first described for flagellates classified in the division Prasinophyta Mohn (Ricketts 1966). MgDVP was identified as a precursor in the synthesis of ChIs a, c\ or C2 (Porra et al 1997) and hence its presence can be expected in extracts of any algal species.…”

Section: Overview Of Haptophyte Pigmentsmentioning

confidence: 99%

Pigment diversity of coccolithophores in relation to taxonomy, phylogeny and ecological preferences

et al. 2004

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SummaryPigment analyses can be employed to study distribution, abundance and composition of natural phytoplankton populations using a chemotaxonomic approach. Cultured haptophytes have played a relevant role in advances in this analytical field and many pigments described in the literature were first detected in members of this algal group. The present chapter provides an historical overview of pigment detection in the Haptophyta and contains new data on their distribution in cultured coccolithophores (some of which not previously cultured), analysed in the context of the EC funded CODENET project. This comparative HPLC study was conducted with the largest (73 monoclonal strains) and most diverse (36 species representing ten families and four orders, including six holococcolithophores) haptophyte sample set ever subjected to a comparative study. The observed extraordinary diversity in the pigment composition (based on ten carotenoids, five polar-and three non-polar chlorophyll types) appeared closely related to current taxonomy and published phylogeny. ChI a and the accessory pigments MgDVP, ChI c2, Ddx, Dtx and p ,p-carotene comprised the common haptophyte pigment load. an evolutionary origin of pigment content, rather than short-term temporal adaptations to environmental conditions.

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Magnesium 2,4-divinylphaeoporphyrin a5 monomethyl ester, a protochlorophyll-like pigment present in some unicellular flagellates

Cited by 48 publications

References 12 publications

Monovinyl and Divinyl Protochlorophyllide Pools in Etiolated Tissues of Higher Plants

Monovinyl and Divinyl Protochlorophyllide Pools in Etiolated Tissues of Higher Plants

Patterns of major photosynthetic pigments in freshwater algae. 2. Dinophyta, Euglenophyta, Chlorophyceae and Charales

Pigment diversity of coccolithophores in relation to taxonomy, phylogeny and ecological preferences

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