Patterns of major photosynthetic pigments in freshwater algae. 2. Dinophyta, Euglenophyta, Chlorophyceae and Charales

Schagerl, Michael; Pichler, Clemens; Donabaum, Karl

doi:10.1051/limn/2003005

Cited by 24 publications

(10 citation statements)

References 77 publications

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“…Likewise, diadinoxanthin which may be produced by diatoms, euglenophytes and dinoflagellates, was detected. However, neoxanthin which is a typical biomarker pigment for euglenophytes (Schagerl et al, 2003) as well as peridinin and diatoxanthin which are biomarker pigments for dinoflagellates (Johansen et al, 1974) were not detected, implying that diadinoxanthin also mainly originated from diatoms. Zeaxanthin was detected in the biofilm, and although it may be found as a minor pigment in green algae, it is primarily a product of cyanobacteria (Brotas & Plante-Cuny, 1998).…”

Section: Biofilm Microphytobenthos (Mpb)mentioning

confidence: 97%

Feeding of biofilm-dwelling nematodes examined using HPLC-analysis of gut pigment contents

et al. 2011

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The natural feeding behaviour of the nematodes Chromadorina bioculata (Schultze in Carus 1857) and Chromadorina viridis (Linstow 1876) was studied in situ, within epilithic biofilms of the Garonne River (France). Based on their feedingtype characteristics and population dynamics, it was hypothesised that these species feed selectively on microphytobenthos (MPB) within the biofilm, and that among MPB groups, diatoms are preferred. Highperformance liquid chromatography (HPLC) was used for separation, identification and quantification of pigments both in nematode guts and in the biofilm. This is the first time that nematode gut pigment contents were examined under natural conditions. Diatoms dominated the MPB which also comprised cyanobacteria and green microalgae. The comparison between chlorophyll a content in nematode guts versus in the biofilm showed that C. bioculata and C. viridis fed opportunistically (non-selectively) on MPB within the biofilm. Only diatom biomarker pigments were found in nematode guts suggesting that they could preferentially fed on diatoms among MPB groups. However, the non-detection of biomarker pigments for other microphyte groups could be also linked to HPLC detection limits. It was estimated that Chromadorina nematodes daily ingested on average 0.03-0.67% of the MPB standing stock. This grazing covered only a small part of their energetic requirements, suggesting that besides MPB they probably also fed on other biofilm food sources. Some considerations on the applicability of the HPLC gut pigment analysis technique for the examination of nematode feeding are also presented.

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Section: Biofilm Microphytobenthos (Mpb)mentioning

confidence: 97%

Feeding of biofilm-dwelling nematodes examined using HPLC-analysis of gut pigment contents

et al. 2011

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“…A considerable body of knowledge is available regarding the pigment composition of specific microalgae cultivated under defined culture conditions (see examples in Table ), the pigments present in certain groups of microalgae (Schagerl and Donabaum , Schagerl et al. , Jeffrey and Wright , Wright and Jeffrey ), the roles of groups of pigments in vivo (Guedes et al. ), and pigment biosynthesis routes (Brown et al.…”

Section: Examples Of Strains For Which the Pigment Composition Is Desmentioning

confidence: 99%

Phototrophic pigment production with microalgae: biological constraints and opportunities

Mulders

Lamers

Martens

et al. 2014

Journal of Phycology

241

126

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There is increasing interest in naturally produced colorants, and microalgae represent a bio-technologically interesting source due to their wide range of colored pigments, including chlorophylls (green), carotenoids (red, orange and yellow), and phycobiliproteins (red and blue). However, the concentration of these pigments, under optimal growth conditions, is often too low to make microalgal-based pigment production economically feasible. In some Chlorophyta (green algae), specific process conditions such as oversaturating light intensities or a high salt concentration induce the overproduction of secondary carotenoids (β-carotene in Dunaliella salina (Dunal) Teodoresco and astaxanthin in Haematococcus pluvialis (Flotow)). Overproduction of all other pigments (including lutein, fucoxanthin, and phycocyanin) requires modification in gene expression or enzyme activity, most likely combined with the creation of storage space outside of the photosystems. The success of such modification strategies depends on an adequate understanding of the metabolic pathways and the functional roles of all the pigments involved. In this review, the distribution of commercially interesting pigments across the most common microalgal groups, the roles of these pigments in vivo and their biosynthesis routes are reviewed, and constraints and opportunities for overproduction of both primary and secondary pigments are presented.

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“…Provided the relationship between these values and those of the bulk organism biomass are known and well constrained, the δ 13 C values of the bulk matter can then be estimated robustly. Algal pigments and fatty acids (FAs) have been used as chemotaxonomic markers for freshwater and marine phytoplankton because these biomolecules vary greatly amongst phytoplankton classes [ 10 , 25 , 26 ]. Analyses of the δ 13 C values of chlorophyll-a have been used to obtain a δ 13 C value for phytoplankton in mixed seston [ 27 , 28 ], and the carbon isotope differences between chlorophyll and whole algal cells appears to be rather constant [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Inferring Phytoplankton, Terrestrial Plant and Bacteria Bulk δ¹³C Values from Compound Specific Analyses of Lipids and Fatty Acids

et al. 2015

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Stable isotope mixing models in aquatic ecology require δ13C values for food web end members such as phytoplankton and bacteria, however it is rarely possible to measure these directly. Hence there is a critical need for improved methods for estimating the δ13C ratios of phytoplankton, bacteria and terrestrial detritus from within mixed seston. We determined the δ13C values of lipids, phospholipids and biomarker fatty acids and used these to calculate isotopic differences compared to the whole-cell δ13C values for eight phytoplankton classes, five bacterial taxa, and three types of terrestrial organic matter (two trees and one grass). The lipid content was higher amongst the phytoplankton (9.5±4.0%) than bacteria (7.3±0.8%) or terrestrial matter (3.9±1.7%). Our measurements revealed that the δ13C values of lipids followed phylogenetic classification among phytoplankton (78.2% of variance was explained by class), bacteria and terrestrial matter, and there was a strong correlation between the δ13C values of total lipids, phospholipids and individual fatty acids. Amongst the phytoplankton, the isotopic difference between biomarker fatty acids and bulk biomass averaged -10.7±1.1‰ for Chlorophyceae and Cyanophyceae, and -6.1±1.7‰ for Cryptophyceae, Chrysophyceae and Diatomophyceae. For heterotrophic bacteria and for type I and type II methane-oxidizing bacteria our results showed a -1.3±1.3‰, -8.0±4.4‰, and -3.4±1.4‰ δ13C difference, respectively, between biomarker fatty acids and bulk biomass. For terrestrial matter the isotopic difference averaged -6.6±1.2‰. Based on these results, the δ13C values of total lipids and biomarker fatty acids can be used to determine the δ13C values of bulk phytoplankton, bacteria or terrestrial matter with ± 1.4‰ uncertainty (i.e., the pooled SD of the isotopic difference for all samples). We conclude that when compound-specific stable isotope analyses become more widely available, the determination of δ13C values for selected biomarker fatty acids coupled with established isotopic differences, offers a promising way to determine taxa-specific bulk δ13C values for the phytoplankton, bacteria, and terrestrial detritus embedded within mixed seston.

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Patterns of major photosynthetic pigments in freshwater algae. 2. Dinophyta, Euglenophyta, Chlorophyceae and Charales

Cited by 24 publications

References 77 publications

Feeding of biofilm-dwelling nematodes examined using HPLC-analysis of gut pigment contents

Feeding of biofilm-dwelling nematodes examined using HPLC-analysis of gut pigment contents

Phototrophic pigment production with microalgae: biological constraints and opportunities

Inferring Phytoplankton, Terrestrial Plant and Bacteria Bulk δ¹³C Values from Compound Specific Analyses of Lipids and Fatty Acids

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