Macrophage Area Content and Phenotype in Hepatic and Adipose Tissue in Patients with Obesity Undergoing Roux‐en‐Y Gastric Bypass

Kristensen, M; Lund, Marie; Hansen, Melissa Voigt; Poulsen, Steen Seier; Ploug, Thorkil; Dela, Flemming; Helge, Jørn Wulff; Prats, Clara

doi:10.1002/oby.21964

Cited by 8 publications

(16 citation statements)

References 39 publications

(64 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conversely, our data confirm that Hep-IR in humans is mainly driven by liver steatosis, as repeatedly reported. 36 The discrepancy with previously published data 26 is likely explained by the different study population. At early stages of metabolic alterations and in the absence of morbid obesity and diabetes, there is no association between derangements of glucose metabolism and macrophage activation in NAFLD patients.…”

Section: Discussionmentioning

confidence: 68%

“…However, it is well known that obesity is characterized by accumulation of pro-inflammatory M1 macrophages in adipose tissue. 26 Hypertrophy and hyperplasia of a dysfunctional adipose tissue evoke cellular stress and necrotic adipocyte cell death, activation of innate immune cells, among which (tissue) macrophages, and production of chemokines such as the chemokine (C-C motif) ligand 2 (CCL2) to recruit monocytes from the bloodstream boosting the release of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6). The final result is an obesity-driven chronic state of low-grade adipose tissue inflammation.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Crosstalk between adipose tissue insulin resistance and liver macrophages in non-alcoholic fatty liver disease

Rosso

Kazankov

Younes

et al. 2019

Journal of Hepatology

135

View full text Add to dashboard Cite

Authors contributions: CR provided data collection, statistical analysis, interpreted data and drafted the manuscript, KK, RY, SE, MM and MS provided the acquisition of data, interpreted data and critically reviewed the manuscript, CB, MG and FS provided the acquisition of data, HJM, MLA, HV, AG and JG interpreted data and critically reviewed the manuscript, EB and HG led the development of the study concept and design, interpreted data, drafted and finalized the manuscript.

show abstract

Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Crosstalk between adipose tissue insulin resistance and liver macrophages in non-alcoholic fatty liver disease

Rosso

Kazankov

Younes

et al. 2019

Journal of Hepatology

135

View full text Add to dashboard Cite

show abstract

“…The question arises, whether or not CD206 is expressed by cells other than macrophages or that possibly our staining was weak. However, Kristensen et al reported similar results in hepatic and adipose tissue …”

Section: Discussionmentioning

confidence: 68%

“…However, Kristensen et al reported similar results in hepatic and adipose tissue. 53 There are a few further proteins described in patients with high-grade OA, of which elevated levels in the synovial fluid and serum are connected to the proinflammatory reaction characteristic for OA. Here, members of the MMP-family, especially MMP-3, have to be listed.…”

Section: Discussionmentioning

confidence: 99%

COMP in the Infrapatellar Fat Pad—Results of a Prospective Histological, Immunohistological, and Biochemical Case–Control Study

Grevenstein

Heilig

Dargel

et al. 2019

Journal Orthopaedic Research

View full text Add to dashboard Cite

Knee osteoarthritis (OA) involves several structures and molecules in the joint, which interact in a pathophysiological process. One of these molecules is the cartilage oligomeric matrix protein (COMP). Elevated COMP levels in the synovial fluid as well as in the serum have been described in OA patients. However, this has not been described in the infrapatellar fat pad (IPFP) tissue before. In this prospective trial, we collected 14 IPFPs from patients with high‐grade OA (mean age 63.8 ± 17.6 years) who underwent total knee replacement (OA group) and from 11 healthy patients (mean age 33.7 ± 14.8 years) who underwent anterior cruciate ligament reconstruction (control group). The presence of macrophages (CD68 and CD206) and proinflammatory cytokines (interleukin 1β [IL‐1β] and IL‐6) was analyzed. Histological and immunohistological examinations as well as immunoblotting analysis for COMP, leptin, and matrix‐metalloproteinase‐3 were performed. The IPFPs of both the OA and control group consisted of adipose tissue and fibrous tissue, and the fibrous tissue showed higher score values than the adipose tissue for COMP staining (intensity as well as stained area) in both groups. Although COMP could be detected in most samples, leptin expression was found only in single specimens. COMP could be detected mostly in the fibrous tissue portion of the IPFP. We speculate that it is involved in a remodeling process taking place in the IPFP during OA. Presence of leptin was irregular in immunohistology, and the control group showed higher scores in case of presence. Interestingly, immunoblotting could detect leptin in all analyzed samples. © 2019 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society J Orthop Res 38:747‐758, 2020

show abstract

“…In accordance with the role of crosstalk among resident liver cells, previous evidence indicated that the hepatic macrophage profile, but not subcutaneous AT macrophage content, is associated with hepatic histology improvement in adult patients who underwent bariatric surgery. 46 Furthermore, hepatic macrophage subsets are main drivers of necroinflammation and fibrosis in human NASH. 32,37,47 The present study showed that the number of TLR4 + macrophages in liver and VAT biopsy results is decreased after LSG and correlates with the improvement in the inflammation of the corresponding tissue.…”

Section: Original Articlesmentioning

confidence: 99%

The Contribution of the Adipose Tissue-Liver Axis in Pediatric Patients with Nonalcoholic Fatty Liver Disease after Laparoscopic Sleeve Gastrectomy

Franchitto

Carpino

Alisi

et al. 2020

The Journal of Pediatrics

View full text Add to dashboard Cite

Objective To evaluate the histopathologic modifications in liver and visceral adipose tissue (VAT), and to correlate these changes with clinical measures, adipokine production, and proinflammatory cytokines in a population of adolescents with obesity with nonalcoholic fatty liver disease (NAFLD) who underwent laparoscopic sleeve gastrectomy (LSG). Study design Twenty adolescents with obesity who underwent LSG and with biopsy-proven NAFLD were included. Patients underwent clinical evaluation and blood tests at baseline and 1 year after the surgical procedure. Liver and VAT specimens were processed for routine histology, immunohistochemistry, and immunofluorescence. Results In adolescents with obesity and NAFLD, hepatic histologic alterations were uncorrelated with VAT inflammation. LSG induced in both liver and VAT tissue histopathology amelioration and macrophage profile modification that were correlated with body mass index and improvement in insulin resistance. The adipokine profile in liver and VAT was associated with weight loss and histologic improvement after LSG. Serum proinflammatory cytokines were correlated with liver and VAT histopathology and IL-1b and IL-6 levels were independently predicted by liver necroinflammatory grade. Conclusions This study suggests a unique adipose tissue/fatty liver crosstalk in pediatric patients. LSG induces a similar pattern of histologic improvement in the liver and in VAT. Besides VAT, our results strengthen the role of the liver in adipocytokine production and its contribution to systemic inflammation in pediatric patients with NAFLD.

show abstract

Macrophage Area Content and Phenotype in Hepatic and Adipose Tissue in Patients with Obesity Undergoing Roux‐en‐Y Gastric Bypass

Cited by 8 publications

References 39 publications

Crosstalk between adipose tissue insulin resistance and liver macrophages in non-alcoholic fatty liver disease

Crosstalk between adipose tissue insulin resistance and liver macrophages in non-alcoholic fatty liver disease

COMP in the Infrapatellar Fat Pad—Results of a Prospective Histological, Immunohistological, and Biochemical Case–Control Study

The Contribution of the Adipose Tissue-Liver Axis in Pediatric Patients with Nonalcoholic Fatty Liver Disease after Laparoscopic Sleeve Gastrectomy

Contact Info

Product

Resources

About