Macro- and microelements determination in children with rotavirus infection

Smiyan, O. I.; Smiyan-Horbunova, K. O.; Лобода, Андрій Миколайович; Popov, Serhii Vitaliiovych; Бында, Татьяна Парфеньевна; Vysotsky, I.Yu.; Січненко, Петро Іванович; Петрашенко, Вікторія Олександрівна; Man'ko, Yu. A.; Kosarchuk, V. V.; Gordienko, Olena

doi:10.14739/2310-1210.2018.3.130456

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…Pathogenetic features of RVI are the study subject for a lot of researchers at the present stage [4][5][6][7]. One of the main pathogenetic mechanisms of rotavirus gastroenteritis is carbohydrate malabsorption syndrome, which develops due to decreased surface activity of enterocyte disaccharides, dysfunction of the sodium-dependent glucose transporter and leads to osmotic diarrhea [4].…”

Section: ключові словаmentioning

confidence: 99%

Pathogenetic role of intestinal microflora in carbohydrate malabsorption syndrome in early-aged children with rotavirus infection

Воробйова

Усачёва

Kaplaushenko

2021

ZMJ

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The aim is to evaluate the effect of metabolic activity of intestinal microflora on laboratory manifestations of carbohydrate malabsorption syndrome in early-aged children with rotavirus infection by studying the main intestinal metabolites – short-chain fatty acids in faeces. Materials and methods. 60 breastfed children with rotavirus infection aged 1–24 months were examined. The total amount of carbohydrates in faecal samples was determined using Benedict’s test, and the detection of short-chain fatty acids in faeces was carried out using liquid chromatography in the dynamics of the disease – on the 3rd, 5th and 10th day. Results. It was established, that the amount of reducing sugars in faeces decreased with the increasing concentration of intestinal microflora metabolites during the entire period of rotavirus infection. In patients with ≤0.5 % level of carbohydrates in faeces, the total content of short-chain fatty acids was the highest and exceeded by 2.0, 1.8 and 1.7 times the indicators of children with Benedict’s test values >0.5 % on the 3rd, 5th and 10th days of the disease, respectively (p < 0.05). A decrease in the metabolic activity of the intestinal microbiota in the above category of children occurred mainly due to a deficiency in the production of acetic and propionic acids (P < 0.05). In the acute period of the disease, an imbalance in the intestinal microbiota infrastructure was noted towards a deficiency of obligate anaerobes that was expressed in a decrease in the anaerobic index (P < 0.05). The severity of these disorders influenced the severity of carbohydrate malabsorption: with an increase in the level of undigested sugars in faeces >0.5 %, the anaerobic index values were 3 and 5 times lower than at a carbohydrate level ≤0.5 % on the 3rd and 5th days of the disease, respectively (P < 0.05). Conclusions. It has been found that early-aged children with rotavirus infection have structural and functional disorders of the intestinal microflora, which influence the severity of carbohydrate malabsorption syndrome. The lower the saccharolytic activity of intestinal bacteria, the more pronounced the manifestations of this syndrome during the entire period of the disease.

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Section: ключові словаmentioning

confidence: 99%

Pathogenetic role of intestinal microflora in carbohydrate malabsorption syndrome in early-aged children with rotavirus infection

Воробйова

Усачёва

Kaplaushenko

2021

ZMJ

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“…При цьому О.І. Сміян із співавторами визначили значне зниження рівня цинку в сироватці крові у дітей з ротавірусною інфекцією [12].…”

Section: результати та обговоренняunclassified

Сравнительная оценка уровня цинка и IgA в сыворотке крови детей с острыми кишечными инфекциями

Usachova¹,

Pechugina²

2021

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Актуальность. Новые рекомендации Всемирной организации здравоохранения по лечению диареи касаются применения цинка (Zn). Zn при диарее улучшает абсорбцию воды в кишечнике, ускоряет регенерацию энтероцитов, увеличивает количество кишечных ферментов, улучшает иммунный ответ, важным звеном которого является иммуноглобулин А (IgA). Целью было определить уровни Zn и IgA в сыворотке крови детей раннего возраста в динамике острой инфекционной диареи (ИД) бактериальной и вирусной этиологии. Материалы и методы. Под наблюдением находились 36 детей в возрасте от 4 месяцев до 15 лет с инфекционной диареей. Дети были разделены на три группы: I — 14 детей в возрасте до двух лет с ИД бактериальной этиологии, II — 9 детей соответствующего возраста с ИД ротавирусной этиологии, III — 13 детей с диареей в возрасте старше двух лет. У детей были определены уровни Zn и IgA в сыворотке крови на 1-й и 5-й день лечения. Результаты. Установлено, что у 19,44 % больных ИД отмечается низкий уровень Zn сыворотки крови в 1-й день стационарного лечения, а у 25 % — выше нормы. У больных I и II группы низкий уровень Zn был у 26,09 %, выше нормы — у 13,04 % (6,78–35,5 мкмоль/л), а у детей группы III — у 11,11 % был ниже нормы и у 66,67 % — высоким (7,9–9,5 мкмоль/л). Разницы в частоте гипоцинкемии у детей раннего возраста с ИД ротавирусной и бактериальной этиологии выявлено не было. Также было определено снижение уровня сывороточного IgA у каждого 8-го больного среди детей раннего возраста в первые дни стационарного лечения при отсутствии относительных изменений в старшей возрастной группе. На 5-й день стационарного лечения произошла нормализация показателей цинка и сывороточного IgА у всех детей. Выводы. Таким образом, у четверти детей раннего возраста в остром периоде ИД вирусной или бактериальной этиологии наблюдается дефицит цинка и недостаточность сывороточного IgА, что требует проведения дальнейших клинико-лабораторных обследований.

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Laboratory signs of carbohydrate malabsorption in early age children with rotavirus infection

Воробйова¹,

Усачова²

2021

Patologìâ

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The aim – to determine the laboratory manifestations of carbohydrate malabsorption syndrome in the dynamics of rotavirus infection in early age children and to identify the factors that influence its severity. Materials and methods. The study included 60 breastfed children aged 1–24 months with rotavirus infection, who underwent pH-metry of feces, semi-quantitative determination of total carbohydrates (Benedict’s method), lactose (Malfatti method) and glucose (test systems Glucophan) in feces on the 2nd, 3rd, 5th, 7th and 10th days of the disease. Results. Carbohydrate malabsorption syndrome was observed in 98.3% of patients. The level of fecal carbohydrates increased on the second-third day to a clinically significant 0.50 [0.20; 1,50] %, reached its maximum in the midst of the disease – from the fifth to the seventh day (0.75 [0.50; 1.65] % and 0.87 [0.40; 1.65] %, respectively), decreasing during convalescence to 0.50 [0.20; 1.50] % (P < 0.05 relative to the 10th day), but remained elevated in 81.7 % of patients (P < 0.05 relative to healthy children). Lactase deficiency and glucose malabsorption (P < 0.05 relative to healthy children) were observed throughout the RVI period. At all stages of RVI there was a strong direct correlation between the total level of carbohydrates and lactose in the feces (on the second, third, fifth, seventh and tenth days r = 0.91; r = 0.86; r = 0.91; r = 0.89, respectively). The age dependence of the severity of laboratory indicators of carbohydrate malabsorption was established. Children in the first 6 months had the highest maximum levels of carbohydrates and fecal lactose – 1.75 [1.00; 2.00] % and 3.50 [2.00; 4.00] + respectively (P < 0.05 relative to 12–24 months children) and significantly higher values of these indicators in the dynamics of RVI (P < 0.05 relative to 12–24 months children) for the second-third, fifth and the tenth day). Children with concomitant food allergies, atopic dermatitis and iron deficiency anemia had 2.0 and 1.5 times higher maximum levels of carbohydrates and lactose in the stool, respectively (P < 0.05), as well as higher values of these indicators in the dynamics of the disease – on the third and fifth days (P < 0.05). Conclusions. The vast majority of young patients with rotavirus infection have carbohydrate malabsorption syndrome, which occurs from the first days of the disease and is most pronounced on the fifth to seventh day (P < 0.05 relative to the 10th day), realized mainly due to lactase deficiency. The most pronounced carbohydrate malabsorption syndrome and lactase deficiency in the dynamics of the disease are present in children of the first six months of life. Factors that increase the severity of carbohydrate malabsorption due to secondary lactase deficiency in the acute period of rotavirus infection are concomitant food allergies, atopic dermatitis and iron deficiency anemia.

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Macro- and microelements determination in children with rotavirus infection

Cited by 3 publications

References 12 publications

Pathogenetic role of intestinal microflora in carbohydrate malabsorption syndrome in early-aged children with rotavirus infection

Pathogenetic role of intestinal microflora in carbohydrate malabsorption syndrome in early-aged children with rotavirus infection

Сравнительная оценка уровня цинка и IgA в сыворотке крови детей с острыми кишечными инфекциями

Laboratory signs of carbohydrate malabsorption in early age children with rotavirus infection

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