Pathogenetic role of intestinal microflora in carbohydrate malabsorption syndrome in early-aged children with rotavirus infection

Воробйова, Н. В.; Усачёва, Е. В.; Kaplaushenko, A. G.

doi:10.14739/2310-1210.2021.5.231265

Cited by 3 publications

(3 citation statements)

References 12 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Один із механізмів такого впливу можна пояснити накопиченням олігоцукридів у просвіті кишечника внаслідок недостатньої їх ферментації кишковою мікрофлорою з підтриманням надалі осмотичного компонента діареї. Так, у попередньому дослідженні показано наявність зворотної кореляції між загальною кількістю кишкових метаболітів (КЖК), величиною АІ та рівнем нерозщеплених цукрів у фекаліях у гострий період РВІ [20].…”

Section: обговоренняunclassified

The influence of the metabolic activity of the intestinal microflora on the severity and duration of rotavirus diarrhea in early-aged children

Воробйова¹,

Усачова²,

Silina³

et al. 2023

Patologìâ

View full text Add to dashboard Cite

The aim is to determine the pathogenetic role of the metabolic activity of the intestinal microflora in the formation of the severity and duration of rotavirus diarrhea in early-aged children. Materials and methods. 60 children aged 1–24 months with RVI were included in the research group. The metabolic activity of the intestinal microflora was determined by detecting short-chain fatty acids (SCFAs) in feces in the dynamics of the disease (the absolute concentration (μmol/l) of acetate, propionate, butyrate, the total concentration of SCFAs and the value of the anaerobic index (AI) on the 3rd, 5th and 10th days of RVI) using high-performance liquid chromatography with LC-MS chromatography Agilent 1260 Infinity HPLC System, USA. Results. From the 5th day of RVI, with a minimally expressed diarrheal syndrome, a higher total pool of SCFA and higher AI values were observed than with moderate-severe and severe diarrhea (p ˂ 0.05 on the 5th and 10th days of RVI). A longer duration of the diarrheal syndrome in children was associated with a decrease in the metabolic activity of sucrolytic intestinal bacteria: patients with diarrhea ≤5 days had 2.4 times higher indicators of the total pool of SCFA, than patients with the duration of diarrhea ≥6 days (p ˂ 0.05). The degree of reduction in the concentration of C3 and the value of AI on the 2–3rd days of RVI was directly correlated with the duration of diarrhea, which increased by 1 day with a decrease in C3 by 20.6 μmol/l or a decrease in AI by 0.05. Conclusions. The influence of the intestinal microflora metabolic deficiency on the severity of diarrhea in early-aged children manifests itself from the 5th day of illness. A decrease in the concentration of propionate and the value of AI on the 2–3rd days of the disease is the earliest indicator of prolonged diarrhea in children with RVI.

show abstract

Section: обговоренняunclassified

The influence of the metabolic activity of the intestinal microflora on the severity and duration of rotavirus diarrhea in early-aged children

Воробйова¹,

Усачова²,

Silina³

et al. 2023

Patologìâ

View full text Add to dashboard Cite

show abstract

“…Дети данной подгруппы имели в 3 и 3,3 раза более высокие показатели пробы Бенедикта (р<0,01), а также в 3 и 2,5 раза более высокие значения пробы Мальфатти (р<0,01; р<0,05 соответственно) на III и V сутки РВИ соответственно, чем у пациентов без аллергии, с постепенным уменьшением данной разницы в течение периода реконвалесценции (p>0,05 на X сутки Читайте нас на сайті: http://med expert.com.ua Відомо, що клінічні прояви синдрому маль абсорбції вуглеводів у дітей залежать від сукуп ної дії багатьох факторів: рівня активності фер менту LPH, кількості лактози, що надходить з їжею, індивідуальної чутливості кишечника та стану кишкової мікрофлори [17]. Так, дове дено, що підвищення вмісту вуглеводів у фека ліях дітей з РВІ як маркера осмотичної діареї є проявом функціональної недостатності саха ролітичних бактерій [16] (чим нижча їх фер ментативна активність, тим виразніші зміни лабораторних показників цього синдрому) [23].…”

Section: нв воробьева запорожский государственный медицинский универс...unclassified

Influencing factors on the severity of clinical and laboratory manifestations of carbohydrate malabsorption syndrome in early-aged children with rotavirus infection

Воробйова¹

2021

MPU

Self Cite

View full text Add to dashboard Cite

Purpose — to determine the role of the lactase gene MCM6 allelic polymorphism 13910 C/T and the concomitant food allergy (FA) and atopic dermatitis (AD) on the formation of carbohydrate malabsorption syndrome manifestations in early-aged children with rotavirus infection (RVI). Materials and methods. 60 children aged 1–24 months with RVI were examined. The determination of single nucleotide polymorphism 13910 C/T of the MCM6 gene was performed by real-time polymerase chain reaction and the content of total Ig E in serum was determined by enzyme immunoassay for all children on the day of hospitalization. Total amount of carbohydrates in feces (Benedict's test) and the level of lactose in feces (Malfatti's test) were also determined in the dynamics of the disease (on the 3rd, 5th, 7th, 10th day). The analysis of the carbohydrate malabsorption syndrome clinical and laboratory signs was carried out in subgroups depending on the genotype 13910 C/T of the MCM6 gene and concomitant allergic pathology. Results. No significant differences were found in the maximum daily frequency of diarrhea, its duration and the frequency of intestinal colic and flatulence registration in children with genotypes C/C, C/T and T/T -13910 of the MCM6 gene (p>0.05). There was no statistically significant difference in the total level of carbohydrates and lactose in feces at all stages of the disease (p>0.05 on the 3rd, 5th, 7th, 10th). Children with concomitant FA and AD had 1.3 and 2 times higher daily frequency of liquid bowel movements during the height of the disease (p<0.05 on the 5th and 7th days, respectively), 1.3 times longer diarrhea (p<0.05), 1.6 and 1.8 times higher incidence of flatulence and intestinal colic (p<0.05). These children had 3 and 3.3 times higher Benedict's test values (p<0.01), as well as 3 and 2.5 times higher Malfatti's test values (p<0.01; p<0.05, respectively) on the 3rd and 5th days of RVI, respectively, than in patients without allergies, with a gradual decrease in this difference during the period of convalescence (p>0.05 on the 10th day). Conclusions. The allelic polymorphism 13910 C/T of the MCM6 gene does not affect the degree of oligosaccharide metabolism disorders in the intestines in early-aged children with RVI. Against the background of concomitant food allergy and atopic dermatitis in children with RVI, there are more pronounced laboratory signs of carbohydrate malabsorption, and, as a consequence, diarrhea more pronounced during the height of the disease with a higher incidence of flatulence and intestinal colic. The research was carried out in accordance with the principles of the Helsinki declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interest was declared by the author. Key words: rotavirus infection, early-aged children, carbohydrate malabsorption syndrome, lactase gene polymorphism, food allergy, atopic dermatitis.

show abstract

“…Збудниками інфекційної діареї можуть бути різні патогени: бактерії, віруси, паразити, гриби, -які визначають особливості перебігу, діагностики та лікування. Останнім часом змінюється структура етіологічних факторів діареї: зменшується частота інфекцій, спричинених «традиційними» збудниками (Shigella, Salmonella), зростає кількість випадків гострої діареї, зумовленої вірусами [7]. Ротавірусний гастроентерит є найчастішою причиною госпіталізацій із діагнозом ГКІ, уражає дітей віком до п'яти років [7,8].…”

unclassified

Influence of zinc supply on the course of infectious diarrhea in children of early age

Pechuhina,

Usachova

2024

Pathologia

View full text Add to dashboard Cite

Aim. Give a comparative characteristic of the course of infectious diarrhea in young children, residents of the Zaporizhzhia region (Ukraine), depending on their provision of zinc (Zn). Materials and methods. The study included 99 children aged 1 to 36 months with manifestations of infectious diarrhea. On the day of hospitalization, immunochromatographic and bacteriological examination of feces was performed. Also, on the first, third and fifth days of hospitalization, a general blood test and the level of Zn in the blood were determined for each patient. Results. It was found that among the examined patients with infectious diarrhea at the onset of the disease, 22 children (22.2 %) had a reduced concentration of Zn in blood serum. In these children, the zinc level ranged from 7.63 to 9.17 μmol/l. It was also noted that the level of Zn in these patients on the 5th day of inpatient treatment rose to the norm of reference indicators. After studying the anamnesis, it was found that 40.9% of children with low Zn content had episodes of acute intestinal infection before this disease. During a comparative analysis of the clinical and laboratory data of patients, it was found that the tendency to more frequent vomiting occurred in patients with Zn deficiency (p = 0.05), that Zn availability had the most significant effect on the expressiveness of the diarrheal syndrome at the onset of the disease and more than a third (n = 8, 36.4 %) of children with Zn deficiency had more than 5 episodes of diarrhea per day, among which every second had more than 10. We did not determine significant differences between the frequency of viral and bacterial causes of diarrhea in the comparison groups. As for complete blood count, patients with Zn deficiency more often had a tendency to blood neutrophilia with a reliably frequent shift of the leukocyte formula to the left (p = 0.003). At the same time, children with a reduced content of Zn in blood serum significantly more often had lymphopenia at the onset of diarrheal disease (p = 0.006). When investigating the effect of Zn deficiency on the course of the disease, we found that in children with reduced Zn, the duration of diarrhea was almost twice as long (8 [6; 9] days vs. 5 [4; 6] in children with normal Zn indicators; p = 0.0001 according to the Mann–Whitney test) and in patients with infectious diarrhea who had Zn deficiency at the onset of the disease, on the seventh day of treatment diarrhea persisted significantly more often (p = 0.03). Such symptoms were accompanied by the persistence of signs of anemia (p = 0.06 on the 5th day) and a shift of the leukocyte formula to the left (p = 0.007) without leukocytosis. Conclusions. Almost 25 % of young children with infectious diarrhea in the Zaporizhzhia region had Zn deficiency. Most likely, Zn deficiency is caused by the peculiarities of its supply with food and its metabolism. In the debut of a diarrheal disease, insufficient supply of Zn is associated with lymphopenia and a shift of the leukocyte formula to the left. Zn deficiency in infectious diarrhea leads to a long (more than 5 days) course of the disease due to prolongation of the diarrheal syndrome.

show abstract

Pathogenetic role of intestinal microflora in carbohydrate malabsorption syndrome in early-aged children with rotavirus infection

Cited by 3 publications

References 12 publications

The influence of the metabolic activity of the intestinal microflora on the severity and duration of rotavirus diarrhea in early-aged children

The influence of the metabolic activity of the intestinal microflora on the severity and duration of rotavirus diarrhea in early-aged children

Influencing factors on the severity of clinical and laboratory manifestations of carbohydrate malabsorption syndrome in early-aged children with rotavirus infection

Influence of zinc supply on the course of infectious diarrhea in children of early age

Contact Info

Product

Resources

About