m-RNA profiling of HSP-70 under different tropical stress conditions in various broilers

Amrutkar, S.A.; Saxena, V.K.; Tomar, SS

doi:10.14202/vetworld.2014.100-107

Cited by 7 publications

(2 citation statements)

References 27 publications

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“…Heat shock proteins(HSPs) are a group of highly conserved proteins synthesized by cells in response to stimuli, Which protect organs from the harmful effects of stress [17] . Currently known including HSP60, HSP70, HSP90, and HSP110 [18].…”

Section: Introductionmentioning

confidence: 99%

Physiological responses reveal the adaptability of different body colors in tuss ah (Antheraea pernyi ) under extreme heat stress

Li,

Chen,

Jiang

et al. 2023

Preprint

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Background The tussah (Antheraea pernyi ) is an important silk-producing economic insect that needs to be reared in the wild. High temperature is a severe environmental factor affecting growth and reducing yield. Differentbody color species of A. pernyi present latitudinal distribution, and the reasons are still poorly understood. Therefore, this study was undertaken on the adaptability of different body colors of A. pernyi in response to extreme thermal temperatures, by detecting the expression of heat shock protein 70 (HSP70) and level of Superoxide dismutase (SOD) and (Catalase) CAT enzyme activities could display differences in different body color species. Results This study revealed that 1. The highest SOD enzyme activity was observed in green-yellow species(Liao Can 527), and the enzyme activity was relatively lowest in yellow species (Fang shan huang). The SOD enzyme activities of the three species revealed remarkable differences after 1 h of heat shock(p<0.05). 2. The highest CAT enzyme activity was observed in green species(Ji qing), three species exhibited significant differences in CAT enzyme activity at 3 h(p<0.05), but the CAT enzyme activity was relatively low in the hemolymph. 3. The mRNA expression levels of HSP70 in the three species elevated to a maximum after 1 h of treatment and then gradually down-regulated. The HSP70 expression of green species was lowest and yellow species was highest in response to exposure to the thermal environment. The expression levels of HSP70 were significantly different among the three species at 2 h after treatment. The findings trending towards that SOD and CAT enzyme activities both showed an increasing trend followed by a decrease were subjected to acute thermal challenge and levels of HSP70 expression presented a decreasing pattern. Correlation analysis revealed that SOD activity was negatively correlated with time, and HSP70 expression presented species dependence. Conclusion These achievements provide evidence that differences in heat resistance of different body colors A. pernyi. Yellow body color is more heat resistant and conforms to low-latitude adaptability, other body colors are relatively weak. In addition, the SOD enzyme activity and HSP70 gene expression of A. pernyiare advantageous in assessing the resistance of species.

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Section: Introductionmentioning

confidence: 99%

Physiological responses reveal the adaptability of different body colors in tuss ah (Antheraea pernyi ) under extreme heat stress

Li,

Chen,

Jiang

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…There is a strong correlation between chicken genotype and HSP70 genotype with the ability of chickens to withstand heat exposure [ 21 ]. Chickens that produce higher HSP70 will be more tolerant when exposed to heat stress [ 22 ]. Heat stress is able to not only increase the expression of HSP70 but also the production of reactive oxygen species (ROS).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Expression of AQP2 and HSP70 in Broiler Kidney Tissue Treated with Salix tetrasperma Roxb. Extract under Heat Exposure

Sugito

Etriwati

Akmal

et al. 2021

The Scientific World Journal

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The administration of plant extracts to broilers may be a way to mitigate the effects of heat stress. The importance of AQP2 and HSP70 compounds in maintaining the homeostasis of the chicken body when it is subjected to heat stress is well established. This study aims to determine the effect of giving the ethanolic extract of the leaves of Salix tetrasperma Roxb. on the immunohistochemical expression of AQP2 and HSP70 in exposed and unexposed broiler kidney tissue. This study used 36 samples of 28-day-old chicken kidneys. Chickens were kept in individual cages, provided with feed and drinking water ad libitum. The design used was a completely randomized design with 6 treatments and 6 replications: (a) chickens were reared in conditions exposed to heat (HS + 0); (b) chickens were reared in conditions exposed to heat and given Salix extract at a dose of 50 mg/L drinking water (HS + 50); (c) chickens were reared under heat-exposed conditions and given Salix extract at a dose of 100 mg/L drinking water (HS + 100); (d) chickens were reared in conditions without exposure to heat (n-HS + 0); (e) chickens were reared in conditions without exposure to heat and given Salix extract at a dose of 50 mg/L drinking water (nHS + 50); and (f) chickens were reared in conditions exposed without exposure to heat and given 100 mg/L drinking water (nHS + 100) of Salix extract. Salix extract was given for 24 hours and was renewed every 6 hours. The results showed that giving Salix extract 100 mg/L in drinking water to chickens exposed to heat (HS + 100) reduced the value of the H/L ratio. Giving Salix extract 50–100 mg/L in drinking water caused an upregulated AQP2 expression; on the other hand, it downregulated HSP-70 expression, in chicken kidney tubules both exposed to heat stress and nonexposed to heat stress. In conclusion, exposure to heat stress in broiler chickens and giving Salix extract can increase the formation of aquaporin 2 compounds and suppress the formation of HSP70.

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Identification of single nucleotide polymorphism in heat shock protein HSP70 and HSP90 after four selection generations in two lines of chickens

Galal

Radwan

2020

Annals of Agricultural Sciences

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m-RNA profiling of HSP-70 under different tropical stress conditions in various broilers

Cited by 7 publications

References 27 publications

Physiological responses reveal the adaptability of different body colors in tuss ah (Antheraea pernyi ) under extreme heat stress

Physiological responses reveal the adaptability of different body colors in tuss ah (Antheraea pernyi ) under extreme heat stress

Immunohistochemical Expression of AQP2 and HSP70 in Broiler Kidney Tissue Treated with Salix tetrasperma Roxb. Extract under Heat Exposure

Identification of single nucleotide polymorphism in heat shock protein HSP70 and HSP90 after four selection generations in two lines of chickens

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