Lysosomale Enzyme in der Oogenese und Follikulogenese des Meerschweinchens

Abstract

In the ovaries of 75 guinea pigs from 42 days of gestational age to adult animals follicles of every developmental stage were investigated by means of histochemistry for the appearance of acid phosphatase, beta-glucuronidase and non-specific esterase in oocytes and follicle epithelial cells. For morphological control semi-thin sections were examined. Already in primordial follicles, oocytes exhibit a beta-glucuronidase and acid phosphatase reaction. Non-specific esterase can be detected in the oocytes and granulosa cells of secondary follicles. The three of them increase in activity during the growth of the follicles, but show no differences in the ontogenesis of the guinea pig. In the follicle epithelium the reaction of non-specific esterase is especially apparent in the cytoplasm of the cells surrounding the so called "Call-Exner-Bodies". The highest activity is seen in the content of these formations whereas the liquor folliculi does not show a considerable activity. Atresia of follicles can be detected both by enzyme histochemistry and visually in semi-thin sections, at least in advanced stages. The boundaries with intact follicles can at first be recognized by comparison of the follicle epithelium.

A contribution to the biogeography and taxonomy of two Anatolian mountain brook newts, Neurergusbarani and N. strauchii (Amphibia: Salamandridae) using ecological niche modeling

“…Biogeography of Neurergus spp. in Turkey revealed that while the Cilo Mountains is a geographic barrier between N. strauchii and N. crocatus (Schmidtler and Schmidtler, 1970), Gül (2019) contributed to this outcome by examining the niche differentiation between these species. The Euphrates River made the same role between N. barani and N. strauchii providing a decisive effect in the separation of these taxa.…”

“…were gathered from literature (Schmidtler and Schmidtler, 1970;Baran and Öz, 1986;Steinfartz et al, 2002;Arıkan et al, 2003;Bogaerts et al, 2006;Pasmans et al, 2006;Özdemir et al, 2009;Schneider and Schneider, 2010;Coşkun et al, 2013;Olgun et al, 2015;Olgun et al, 2016;Akman et al, 2018;Yıldız et al, 2018;Rancilhac et al, 2019) (Appendix 1; Figure 1). We downloaded bioclimatic variables in km resolutions for current and future distributions (30 arc-second) from WorldClim as v. 1.4 (Hijmans et al, 2005; available at www.worldclim.org) (Appendix 2).…”

“…For instance, the Kubbe Mountain population from the southeast of Malatya was recommended by Öz (1994) as the new subspecies Neurergus strauchii barani. The nominate subspecies, N. s. strauchii has a wider distribution area and spreads from the east of the Euphrates to the west and south of Lake Van (Schmidtler and Schmidtler, 1970;Baran and Öz, 1986;Pasmans et al, 2006;Schneider and Schneider, 2010;Çoşkun et al, 2013Çoşkun et al, , Olgun et al, 2016Yıldız et al, 2018), while N. s. barani is distributed in the west of the Euphrates River Pasmans et al, 2006;Rancilhac et al, 2019). Along with these locality record studies, the preliminary molecular-based phylogenetic studies revealed that there might have relatively significant genetic differentiation between these taxa (Steinfartz et al, 2002;Pasmans et al, 2006).…”

Cytochemical characterization of the endomembranous system during oocyte secondary growth in Serrasalmus spilopleura (Teleostei, Characiformes, Characidae)

“…1981; Busson‐Mabillot 1984; Rune 1984; Raikhel and Lea 1986). AcPase reactivity along the nucleus–Golgi complex, found in mature oocytes but apparently not associated with lysosomal compartments (Rune 1984), has been correlated with the storage of nutritional material (Korfsmeier 1979, 1980; Schmidtler 1980). The extralysosomal membrane‐bound AcPase, detected along the nuclear envelope, endoplasmic reticulum and Golgi cisternae, is supposedly involved with the intense build‐up of coated transport vesicles and probably associated to the highlighting of the biosynthetic activity (Shing and Atwal 1997).…”

Ultrastructural Localization of Acid Phosphatase in Germ Cells of Chick Embryo Left Ovary

“…The results of various authors [Guraya, 1979;Korfsmeier, 1978Korfsmeier, , 1979Schmidtler. 1980] indicate a probable metabolic role of acid phosphatase in the oocytes of some vertebrates.…”