Cytochemical characterization of the endomembranous system during oocyte secondary growth in Serrasalmus spilopleura (Teleostei, Characiformes, Characidae)

Abstract

Guimarães, A. C. D. and Quagio-Grassiotto I. 2008. Cytochemical characterization of the endomembranous system during oocyte secondary growth in Serrasalmus spilopleura (Teleostei, Characiformes, Characidae). -Acta Zoologica (Stockholm) 89 : 37-46The endomembranous system of Serrasalmus spilopleura oocyte secondary growth was analysed using structural and ultrastructural cytochemical techniques. In vitellogenic oocytes, the endoplasmic reticulum components, the nuclear envelope intermembranous space, some Golgi dictiossomes, lysosomes, yolk granules, regions of the egg envelope and sites of the follicle cells react to acid phosphatase detection (AcPase). The cortical alveoli, some heterogeneous cytoplasmic structures, regions of the egg envelope, and sites of the follicle cells are strongly contrasted by osmium tetroxide and zinc iodide impregnation (ZIO). The endoplasmic reticulum components, some vesicles, and sites of the follicle cells also react to osmium tetroxide and potassium iodide impregnation (KI). The biosynthetic pathway of lysosomal proteins, such as acid phosphatase, required for vitellogenesis, involves the endoplasmic reticulum, Golgi complex, vesicles with inactive hydrolytic enzymes, and, finally, lysosomes. In S. spilopleura oocytes at secondary growth, the endomembranous system takes part in the production of the enzymes needed for vitellogenesis, and in the metabolism of yolk exogenous components (AcPase detection). The endomembranous system compartments also show reduction capacity (KI reaction) and are involved in the metabolism of proteins rich in SH-groups (ZIO reaction).

Asymmetry in the cytoplasm of oocytes of largescale yellowfish Labeobarbus marequensis Smith 1841 (Teleostei: Cypriniformes: Cyprinidae)

“…Multilamellar bodies present in oocytes of L. marequensis during Stage 2 possibly serve as organelles in which membranes necessary to increase the size of oocytes are stored. Such bodies have also been described in previtellogenic and in vitellogenic oocytes of the pirambeba, Serrasalmus spilopleura, and arise as a result of recycling of intracellular components in autophagosomes (Guimarães & Quagio‐Grassiotto, 2005, 2008).…”

Acid and alkaline phosphatase localization in the digestive tract mucosa of the Hemisorubim platyrhynchos

“…Furthermore, acid phosphatase reaction also occurred in the nucleus of cells of the mucosa layer throughout the digestive tract, primarily in gastric epithelial cells, oxynticopeptic cells and enterocytes. Several researchers have reported the presence of nuclear acid phosphatase in fish (Calzada et al, 1998;Nóbrega and Quagio-Grassiotto, 2007;Guimarães and Quagio-Grassiotto, 2008). According to Nóbrega and Quagio-Grassiotto (2007), acid phosphatase reaction in the nucleus of Leydig cells of Serrasalmus spilopleura was interpreted as a variation of the nuclear functional state.…”

“…In gastric glands of H. platyrhynchos, acid phosphatase was detected in lysosomes, lamellar bodies and the vesiculotubular system in the oxynticopeptic cells. Lysosomes are the cell's main digestive compartment to which all sorts of macromolecules are delivered for degradation (Lüllmann-Rauch, 2005), while the multilamellar bodies are residual bodies of phagosomes, which result from high metabolic activity of cells (Guimarães and Quagio-Grassiotto, 2008). However, Kozarić et al (2004) correlated acid phosphatase in the gastric glands of Merluccius merluccius with the secretion process.…”