Lysis of<i>Escherichia coli</i>cells induced by bacteriophage T4

Tarahovsky, Yury S.; Ivanitsky, G. R.; Khusainov, A. A.

doi:10.1111/j.1574-6968.1994.tb07164.x

Cited by 37 publications

(22 citation statements)

References 10 publications

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“…Therefore, we performed the same experiment at a MOI of 1,000, but all E. coli cells were lysed (data not shown). This result was probably due to lysis-fromwithout (Tarahovsky et al 1994), a phenomenon that is peculiar to the T4 phage and due to adsorption of a large number of T4 particles to the cell wall. Therefore, it was concluded that nucleic acid stain labeling using the penetration method is difficult to employ for the FLP method.…”

Section: Resultsmentioning

confidence: 83%

“…The cell lysis ratio increased with an increasing EdU dosage. Because the cell lysis may have occurred due to lysisfrom-without (Tarahovsky et al 1994), we hypothesized that the amount of EdU-labeled phage was not calculated correctly. Therefore, to evaluate EdU-labeled T4 phage numbers, we prepared EdU-labeled T4 phages using the same procedure used for non-labeled T4 phage particles, which displayed an approximately 10 9 PFU/ml concentration on E. coli lawn agar plates.…”

Section: Resultsmentioning

confidence: 99%

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A method for evaluating the host range of bacteriophages using phages fluorescently labeled with 5-ethynyl-2′-deoxyuridine (EdU)

Ohno¹,

Okano²,

Tanji³

et al. 2012

Appl Microbiol Biotechnol

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Section: Resultsmentioning

confidence: 83%

Section: Resultsmentioning

confidence: 99%

A method for evaluating the host range of bacteriophages using phages fluorescently labeled with 5-ethynyl-2′-deoxyuridine (EdU)

Ohno¹,

Okano²,

Tanji³

et al. 2012

Appl Microbiol Biotechnol

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“…For the detection of both culturable and VBNC E. coli O157:H7 cells, a high MOI is desirable to enable strong visualization of the cells. Lysis from without (LO) is peculiar to the T4 phage (20). LO is lysis due to adsorption of a large number of T4 particles on the cell wall and occurs at MOIs of Ͼ20.…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Escherichia coli O157:H7 by Using Green Fluorescent Protein-Labeled PP01 Bacteriophage

Oda

Morita

Unno

et al. 2004

Appl Environ Microbiol

144

102

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A previously isolated T-even-type PP01 bacteriophage was used to detect its host cell, Escherichia coli O157:H7. The phage small outer capsid (SOC) protein was used as a platform to present a marker protein, green fluorescent protein (GFP), on the phage capsid. The DNA fragment around soc was amplified by PCR and sequenced. The gene alignment of soc and its upstream region was g56-soc.2-soc.1-soc, which is the same as that for T2 phage. GFP was introduced into the C-and N-terminal regions of SOC to produce recombinant phages PP01-GFP/SOC and PP01-SOC/GFP, respectively. Fusion of GFP to SOC did not change the host range of PP01. On the contrary, the binding affinity of the recombinant phages to the host cell increased. However, the stability of the recombinant phages in alkaline solution decreased. Adsorption of the GFP-labeled PP01 phages to the E. coli cell surface enabled visualization of cells under a fluorescence microscope. GFP-labeled PP01 phage was not only adsorbed on culturable E. coli cells but also on viable but nonculturable or pasteurized cells. The coexistence of insensitive E. coli K-12 (W3110) cells did not influence the specificity and affinity of GFP-labeled PP01 adsorption on E. coli O157:H7. After a 10-min incubation with GFP-labeled PP01 phage at a multiplicity of infection of 1,000 at 4°C, E. coli O157:H7 cells could be visualized by fluorescence microscopy. The GFP-labeled PP01 phage could be a rapid and sensitive tool for E. coli O157:H7 detection.

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“…To obtain variants with increased oxidation activity toward glyphosate, an enzyme-coupled colorimetric assay combined with cell lysis induced by bacteriophase T7 was used for highthroughput selection [8,22,33,36]. Transformants of the library were cultivated in the 96-deep well plates containing 600 L of LB medium and ampicillin (100 g/mL) overnight at 37 • C, and every clone had a corresponding backup on LB agar plate.…”

Section: Screening Of Mutants With Improved Activitymentioning

confidence: 99%

Improvement of glycine oxidase by DNA shuffling, and site-saturation mutagenesis of F247 residue

Yao

Lin

et al. 2015

International Journal of Biological Macromolecules

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Lysis ofEscherichia colicells induced by bacteriophage T4

Cited by 37 publications

References 10 publications

A method for evaluating the host range of bacteriophages using phages fluorescently labeled with 5-ethynyl-2′-deoxyuridine (EdU)

A method for evaluating the host range of bacteriophages using phages fluorescently labeled with 5-ethynyl-2′-deoxyuridine (EdU)

Rapid Detection of Escherichia coli O157:H7 by Using Green Fluorescent Protein-Labeled PP01 Bacteriophage

Improvement of glycine oxidase by DNA shuffling, and site-saturation mutagenesis of F247 residue

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