Lymphocytophilic Antibody Titer as a Correlative Assay for the Immunosuppressive Activity of Antilymphocytic Serum

The Weizmann Institute of Science, Rehovot Solubilization of lymphocyte and thymocyte antigens by a reversible chemical modificationAntigenic components of the particulate subcellular fraction from either rat thymocytes or lymph node lymphocytes can be obtained in a soluble form by reacting the membrane fraction with citraconic anhydride. As a result of citraconylation, 60-70 % of the proteins became soluble under physiological conditions and this soluble material was antigenically almost inactive. However, after decitraconylation at pH 5 and room temperature, the solubIe protein possessed antigenic activity (10-20 % of the original antigenic potency .of the lymphocytes) as tested by inhibition of the cytotoxic effect of heterologous anti-lymphocyte serum on lymphocytes and by other assays. The soluble antigenic components thus obtained from rat thymocytes induced the formation of anti-thymocyte serum when injected into rabbits. This antiserum was found to be active against thymocytes in the cytotoxic and opsonization assays and to suppress the rejection of skin allografts.

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“…Controls were similarly treated with NRS. Opsonization titers were expressed as proposed by Bach et al [29].…”

Section: Opsonization Assaymentioning

confidence: 99%