1971
DOI: 10.1002/eji.1830010504
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Solubilization of lymphocyte and thymocyte antigens by a reversible chemical modification

Abstract: The Weizmann Institute of Science, Rehovot Solubilization of lymphocyte and thymocyte antigens by a reversible chemical modificationAntigenic components of the particulate subcellular fraction from either rat thymocytes or lymph node lymphocytes can be obtained in a soluble form by reacting the membrane fraction with citraconic anhydride. As a result of citraconylation, 60-70 % of the proteins became soluble under physiological conditions and this soluble material was antigenically almost inactive. However, af… Show more

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Cited by 18 publications
(4 citation statements)
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References 29 publications
(8 reference statements)
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“…Preparation of keratin Prekeratin-enriched preparations, from bovine muzzle, containing the major epidermal polypeptides I -VII (Franke et al, 1978bSchiller et al, 1982) were obtained as described (Franke et al, 1981c Cellfusion and growth Three days after the last boost, spleens were removed and 100 x 106 cells from each individual spleen were fused with 20 x 106 NSO/1 myeloma line (Galfre and Milstein, 1981) kindly provided by C.Milstein (MRC, Cambridge, UK) using 41 7o polyethylene glycol 1500 (Serva, Heidelberg, FRG) as described previously (Eshhar et al, 1979). Following fusion, cells were distributed into six microplates (96 wells each) at a concentration of 5 x 10" viable cells/well.…”
Section: Methodsmentioning
confidence: 99%
“…Preparation of keratin Prekeratin-enriched preparations, from bovine muzzle, containing the major epidermal polypeptides I -VII (Franke et al, 1978bSchiller et al, 1982) were obtained as described (Franke et al, 1981c Cellfusion and growth Three days after the last boost, spleens were removed and 100 x 106 cells from each individual spleen were fused with 20 x 106 NSO/1 myeloma line (Galfre and Milstein, 1981) kindly provided by C.Milstein (MRC, Cambridge, UK) using 41 7o polyethylene glycol 1500 (Serva, Heidelberg, FRG) as described previously (Eshhar et al, 1979). Following fusion, cells were distributed into six microplates (96 wells each) at a concentration of 5 x 10" viable cells/well.…”
Section: Methodsmentioning
confidence: 99%
“…Positively reacting mice (determined by RIA, see below) were injected again, 3 weeks after the challenge injection and 3 days later their spleens were removed and used for fusion. The preparation of hybridomas and their maintenance were carried out according to Eshhar et al (1979). The myeloma used for fusion was NSO (Galfre and Milstein, 1981), which were kindly supplied by Dr. Z.Eshhar from our Department.…”
Section: Immunochemical Reagentsmentioning
confidence: 99%
“…In LPS-stimulated B lymphocytes there was a 25to 80-fold increase in ,t heavy chain mRNA (Yuan and Tucker, 1984;Lamson and Koshland, 1984;Berger, 1986). Pre-B and B cell lines have 10-100 times less A heavy chain mRNA than do plasmacytomas and hybridomas (Perry and Kelley, 1979;Eshhar et al, 1979;Perry et al, 1981;Nelson et al, 1983;. This variation in mRNA level could, in principle, result from different rates of transcription at the heavy chain locus, but apparently it does not.…”
Section: Introductionmentioning
confidence: 99%