Detection of a cytokeratin determinant common to diverse epithelial cells by a broadly cross-reacting monoclonal antibody.

Abstract

A monoclonal antibody derived from a mouse immunized with bovine epidermal prekeratin has been characterized by its binding to cytoskeletal polypeptides separated by one‐ or two‐dimensional gel electrophoresis and by immunofluorescence microscopy. This antibody (KG 8.13) binds to a determinant present in a large number of human cytokeratin polypeptides, notably some polypeptides (Nos. 1, 5, 6, 7, and 8) of the ‘basic cytokeratin subfamily’ defined by peptide mapping, as well as a few acidic cytokeratins such as the epidermis‐specific cytokeratins Nos. 10 and 11 and the more widespread cytokeratin No. 18. This antibody reacts specifically with a wide variety of epithelial tissues and cultured epithelial cells, in agreement with previous findings that at least one polypeptide of the basic cytokeratin subfamily is present in all normal and neoplastic epithelial cells so far examined. The antibody also reacts with corresponding cytokeratin polypeptides in a broad range of species including man, cow, chick, and amphibia but shows only limited reactivity with only a few rodent cytokeratins. The value of this broad‐range monoclonal antibody, which apparently recognizes a stable cytokeratin determinant ubiquitous in human epithelia, for the immunohistochemical identification of epithelia and carcinomas is discussed.

Proteolytic modification of acidic and basic keratins during terminal differentiation of mouse and human epidermis

“…The supernatant was dialysed against citrate buffer (30vol., 6 "C) and keratin was isolated by serial isoelectric precipitation (as for prekeratin above). Alternatively, keratin extracts in Tum buffer were dialysed against 5 mM Tris/HCl pH 7.5 containing 0.1 % mercaptoethanol and 1 mM EDTA (30 vol., 6 "C) and the renatured filaments (see [5,9,131) were collected by centrifugation (3000 rpm, 5 min, 6 "C). Keratin was also isolated from pure stratum corneum preparations by overnight extraction with 2 % sodium dodecyl sulfate (SDS and 2 % mercaptoethanol in 50mM Tris/HCl, pH 7.5 Cytoskeletal preparations were made from whole epidermis as described previously [29,44,451 and from fractions of epidermis enriched in either basal, spinous or granular cells.…”

Distribution of Cytokeratin Polypeptides Detected by Monoclonal Antibodies K8.13 and K8.12 in the Fetal Bovine Ruminal Epithelium.

“…Sci. 61(3): 261-265, 1999 ruminal epithelium using two mAbs ; i.e., MAb K8.13 raised against the stratified epithelial type CK polypetides [10,13,14], and mAb K8.12 against the non-stratified hyperproliferative epithelial type CK polypeptides [10,20]. These mAbs recognize the broad-range of CK polypeptides in species including man, cow, chick and amphibia, but show only limited reactivity with only a few rodent CK polypeptides [10].…”

“…These mAbs recognize the broad-range of CK polypeptides in species including man, cow, chick and amphibia, but show only limited reactivity with only a few rodent CK polypeptides [10].…”

Monoclonal antibody to human skin basal cells. Immunoelectron microscopic studies on tissue specific cross-reactivity.

“…Several monoclonal antibodies (MoAbs) that react with epidermal components have been established and used for dermatological research; they recognize cytoskeltal components (3)(4)(5)18), desmosomes (13) of the keratinocytes, Langerhans cells (7,11), Merkel cells (15), or the epidermolysis bullosa acquisita antigen (14). Some of these MoAbs have diagnostic values, and some are useful for examining whether the skin and other organs share common antigens or not.…”