Lymphocyte Subsets in Repeated Apheresis Donors.

Abstract

To investigate the influence of apheresis on lymphocyte subsets, we compared lymphocyte subsets of repeated apheresis donors (RADs) with those of nondonor controls (NDCs).Males were divided into three age groups; group A (age range 19-34 years, RADs; n=19,30+ 11 donations/2y, NDCs; n=16), group B (35-54 years, RADs; n=16, 32+10 donations/2y, NDCs; n=20) and group C (55-67 years, RADs; n14, 32+8 donations/2y, NDCs; n=8). Females were divided into two age groups; group D (19-34 years, RADs; n=14, 23+8 donations/2y, NDCs; n= 18) and group E (35-62 years, RADs; n=12, 22+11donations/2y, NDCs; n=7). In lymphocyte subsets, CD3+T cells, CD4+cells, CD8+ cells, CD19+B cells and CD56+CD3-natural killer (NK) cells were analyzed by flow cytometry and the CD4/CD8 ratio was calculated. The total white blood cell count (TWBC) and absolute lymphocyte count (ALC) were determined using an automatic cell counter. The data of RADs were compared to those of NDCs in each group.In groups A, B and D, there were no significant differences in the percentages of lymphocyte subsets, or in ALCs or TWBCs. In group C, the percentage and absolute numbers of CD4+(RADs; 39.2+7.3%, NDCs; 47.3+4.1%, p<0.01, RADs; 684+185/u1, NDCs; 898+256/u1, p<0.05) and CD4/CD8 ratio (RADs; 1.65+0.74, NDCs; 2.28+0.37, p <0.05) were significantly decreased in RADs. In group D, the percentage and the absolute numbers of CD3+T cells (RADs; 68.5+5.9%, NDCs; 73.9+5.0%, p<0.02, RADs; 1, 162+259/ul, NDCs; 1,372+273/ul, p< 0.05) were significantly decreased in RADs. However, these findings indicate that repeated apheresis did not seriously affect lymphocyte subsets of RADs, since both the percentages and absolute numbers of lymphocyte subsets in RADs were within normal ranges.

An Ab Initio Molecular Dynamics Study of Bioactive Phosphate Glasses

“…The effect of calcium ions on the structure of the glass is therefore to increase the rigidity of the phosphate PÀOÀP interconnec-tivity, which could be interpreted by the more rigid coordination shell of calcium [47] compared to that of sodium. [48,49] This increased rigidity of the phosphate network with the addition of calcium content could be linked to the observed slower solubility of glasses with higher CaO content. [10] Figure 4 reports the decomposition of the PÀO RDFs into the BO and NBO components.…”

DNA‐binding properties of the overexpressed recombinant estrogen receptor α

“…The following buffers were used (7,8) leupeptin (Boehringer Mannheim Co., Mannheim, Germany), antipain (Sigma Co.), chymostatin (Sigma Co.), and soybean trypsin inhibitor (Boehringer Mannheim Co.) were designated as TGMPI and TEGMPI, respectively. TGMPI containing 10 mM sodium molybdate was designated as TGMPIMo.…”

“…The soluble receptor fraction from COS-7 cells transfected with pSV2RcER was prepared witln TGMPIMo. Ammonium sulfate was added to the cytosol at 40 % saturation as previouly described (7,8). The resulting precipitate was collected, resuspended and dialyzed against TGMPIMo (designated as cMoERs).…”

“…However, not only are the tertiary and subunit structures of the receptors unclear, but also interactions of the receptor with DNA and of the DNA-receptor complex with transcription initiation machinery (6,7,8). In attempting to elicit more details of the action mechanisms of estrogens and antiestrogens through elucidation of the DNA-binding state of the receptors, lkeda and Kusaka (7) found that the nuclear estradiol (E2, an estrogen)-, ICt164,384 (ICL a pure antiestrogen)and 4-hydroxytamoxifen (OHT, an antiestrogen )-receptor complexes from rat uterus (rE2ERn, rlC1ERn and rOHTERn, respectively) were cross-linked with 5-bromo-2'-deoxyuridine substituted ERE as the receptor dimer by UV irradiation.…”

“…There was a discrepancy between observations by rMoERs (7) and tile estrogen receptor expressed in COS-1 cells in the absence of ligands (9).…”