Estrogen receptor cr was overexpressed in COS-7 cells by transformation of an expression vector with the full length open reading frame of the receptor 0t. The nuclear estradiol-receptor complex and the soluble receptor from the COS-7 cells were cross-linked with an estrogen response element (ERE), which was substituted with 5-bromo-2'-deoxyuridine (BrdUVRE), as the receptor dimers by UV irradiation. In gel retardation analysis, the specific bindings of both nuclear and soluble receptors to ERE were decreased with increasing of KCI concentration compared with 0.1 M KC1. The ionic interactions of both receptors with ERE are thought to be similar.
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