Lyme Disease Spirochetes in Ticks from Northeastern China

Takada, Nobuhiro; Ishiguro, Fubito; Fujita, Hiromi; Wang, Haipeng; Wang, Jichun; Masuzawa, Toshiyuki

doi:10.2307/3284712

Cited by 33 publications

(22 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, our findings reconfirmed a strong affinity between birds and B. garinii. Although not many Korean or Chinese borreliae have been clearly characterized, most Korean and northeastern Chinese strains isolated in previous surveys (11,14,34) were identified as B. garinii with pattern B or pattern C or B. afzelii with pattern D (14, T. Masuzawa, unpublished data); only two strains, 935T and ChY13p, were identified as having pattern R. Such a unique pattern had not been observed previously among isolates in Japan and far-eastern Russia. Our findings revealed that strains Fi14f and Fi24f, characterized in the pattern R group, were closely related to strains 935T in Korea and ChY13p in China, respectively.…”

Section: Discussionmentioning

confidence: 79%

See 1 more Smart Citation

Prevalence of Lyme Disease Borrelia spp. in Ticks from Migratory Birds on the Japanese Mainland

Ishiguro¹,

Takada

Masuzawa

et al. 2000

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Borrelia sp. prevalence in ticks on migratory birds was surveyed in central Japan. In autumn, a total of 1,733 birds representing 40 species were examined for ticks. A total of 361 ticks were obtained from 173 birds of 15 species, and these ticks were immature Haemaphysalis flava (94.4%), Haemaphysalis longicornis, Ixodes columnae, Ixodes persulcatus, Ixodes turdus, and an unidentified Ixodes species. Of these, 27 juveniles of H. flava on Turdus pallidus, Turdus cardis, or Emberiza spodocephala, 2 juveniles of I. persulcatus on T. pallidus, and 1 female H. flava molted from a T. pallidus-derived nymph were positive for the presence of Borrelia by Barbour-Stoenner-Kelly culture passages. In spring, a total of 16 ticks obtained from 102 birds of 21 species were negative for the spirochete. Isolates from 15 ticks were characterized by 5S-23S rRNA intergenic spacer restriction fragment length polymorphism analysis; all isolates were identified as Borrelia garinii with pattern B/B based on the previous patterning. According to the intergenic spacer sequences, 2 of 15 isolates, strains Fi14f and Fi24f, were highly similar to B. garinii strains 935T of Korea and ChY13p of Inner Mongolia, China, respectively. These findings indicate that Lyme disease-causing B. garinii may have been introduced to Japan by migratory birds from northeastern China via Korea. Additionally, a case of transstadial transmission of B. garinii from nymph to adult H. flava suggests that the infected H. flava may transmit Borrelia to large animals.Lyme disease is primarily caused by three genomic species, Borrelia burgdorferi sensu stricto, Borrelia garinii, and Borrelia afzelii (2, 4). B. garinii and B. afzelii are widely distributed from Europe to the Far East including Japan, while B. burgdorferi sensu stricto is prevalent in North America and has been confirmed in part of Europe. B. burgdorferi sensu lato is mainly transmitted by some tick species of the Ixodes ricinus complex, and these ticks infest both mammals and birds (1,9,10,13,19,25).Concerning the prevalence of Borrelia in ticks feeding on birds, B. garinii has been isolated from Ixodes persulcatus on Emberiza spodocephala and Turdus chrysolaus in Hokkaido, Japan (20, 24), and B. burgdorferi sensu stricto, B. garinii, B. afzelii, and Borrelia andersonii have been isolated from Ixodes dentatus, I. ricinus, Ixodes scapularis, Ixodes uriae, and other ticks, which infest a large number of bird species in Europe and North America (6,13,15,27,28,30). Furthermore, Haemaphysalis leporispalustris, detected on some bird species, was reported to be a reservoir of B. burgdorferi sensu stricto in North America (13,26).B. afzelii is transmitted between I. persulcatus and field rodents, and B. garinii is transmitted between I. persulcatus and migratory birds or rodents, in Hokkaido, Japan (24, 25). However, there has not been a survey of Borrelia in migratory birds which travel directly between the Asiatic continent and Japan. In this survey, we examined the Borrelia prevalence in juvenile ticks remo...

show abstract

Section: Discussionmentioning

confidence: 79%

“…Ticks were removed with forceps and placed in separate glass vials containing moist sanitary cotton. The stage and species of each tick were identified as described by Takada (34).…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Lyme Disease Borrelia spp. in Ticks from Migratory Birds on the Japanese Mainland

Ishiguro¹,

Takada

Masuzawa

et al. 2000

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…A previous study reported 64.4% B. garinii and 35.6% B. afzelii based on a PCRrestriction fragment length polymorphism analysis of 45 B. burgdorferi isolates from this area. 18 Whether the coinfection was associated with B. burgdorferi genospecies remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

“…[13][14][15][16] Northeastern China is highly endemic for Lyme disease, 17 where the infection rate of B. burgdorferi in adult I. persulcatus ticks can be as high as 44.1%. 18 Recently, the presence of A. phagocytophila in ticks from the region has been reported. 12 Therefore, an extensive study was carried out to confirm the previous findings, and to investigate coinfection of I. persulcatus ticks with A. phagocytophila and B. Burgdorferi.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Anaplasma Phagocytophila and Borrelia Burgdorferi in Ixodes Persulcatus Ticks From Northeastern China

Cao

Zhao

Zhang

et al. 2003

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. A total of 1,345 Ixodes persulcatus ticks collected from northeastern China were investigated for the presence of Anaplasma phagocytophila and Borrelia burgdorferi by a nested polymerase chain reaction (PCR). Sixty-two (4.6%) ticks were positive for A. phagocytophila and 454 (33.8%) were positive for B. burgdorferi. Seven (0.5%) were coinfected with both agents. Sequence analysis of 919-basepair PCR amplicons revealed three types of A. phagocytophila. Type 1 was identical to the published sequences of A. phagocytophilas responsible for human granulocytic ehrlichiosis (HGE). The other two variants differed from the HGE agent sequence at one and four positions, respectively. These findings imply that infection with A. phagocytophila poses a potential health threat to both humans and animals in northeastern China, and that ehrlichiosis should be considered in the differential diagnosis of febrile patients with a history of tick bite, particularly when clinical manifestations are atypical for Lyme disease.

show abstract

“…After that, scientific papers using the invalid name have been gradually increased (Takada et al, 1998;Fujita et al, 2000;Yoshimoto et al, 2009;Yokoyama et al, 2012;Tagawa et al, 2013), but a few researchers used names of H. japonica and H. jezoensis for the same organism (Ozawa and Kadosaki, 1996;Ito and Takahashi, 2001). Such a confused and strange situation is still continuing, particularly in Hokkaido.…”

Section: Introductionmentioning

confidence: 99%

Haemaphysalis japonica, Haemaphysalis jezoensis and ^|^#8220;Haemaphysalis douglasi^|^#8221; (Acari: Ixodidae): Which tick is distributed in Hokkaido?

Nakao

Ito

2014

Jap. J. Sanit. Zool.

View full text Add to dashboard Cite

As a general view, the ixodid tick Haemaphysalis japonica is distributed in Japan (Kyushu, Honshu and Hokkaido islands) and in a continental area including Russia Primorsky Krai, eastern China and the Korean Peninsula. The continental population is treated as a subspecies, Haemaphysalis japonica douglasi. The Hokkaido population was once named Haemaphysalis jezoensis, but synonymized with H. japonica. An irregular taxonomic revision, however, elevated H. japonica douglasi to specific rank for the continental and Hokkaido populations. The resultant "Haemaphysalis douglasi" sensu Kitaoka is obviously invalid because of the lack of taxonomic literatures about its specific status. Even in the present time, the invalid name has yet been used for tick identification in Hokkaido. In this study, nucleotide sequences of mitochondrial DNA (large subunit ribosomal RNA gene) and nuclear DNA (internal transcribed spacer 2) were compared between adult samples of H. japonica from Honshu and those of "H. douglasi" from Hokkaido. The target sequences of the Hokkaido samples were completely identical with those of the Honshu samples, indicating that the elevation of H. japonica douglasi to specific rank or the resurrection of H. jezoensis is an inadequate treatment. The result clearly shows that H. japonica is distributed in Hokkaido.

show abstract

Lyme Disease Spirochetes in Ticks from Northeastern China

Cited by 33 publications

References 1 publication

Prevalence of Lyme Disease Borrelia spp. in Ticks from Migratory Birds on the Japanese Mainland

Prevalence of Lyme Disease Borrelia spp. in Ticks from Migratory Birds on the Japanese Mainland

Prevalence of Anaplasma Phagocytophila and Borrelia Burgdorferi in Ixodes Persulcatus Ticks From Northeastern China

Haemaphysalis japonica, Haemaphysalis jezoensis and ^|^#8220;Haemaphysalis douglasi^|^#8221; (Acari: Ixodidae): Which tick is distributed in Hokkaido?

Contact Info

Product

Resources

About