LX Loaded Nanoliposomes Synthesis, Characterization and Cellular Uptake Studies in H<SUB>2</SUB>O<SUB>2</SUB> Stressed SH-SY5Y Cells

Abstract

In this study, we report the cellular uptake studies of novel LX loaded nanoliposomes in H2O2 stress SH-SY5Y Cells synthesized by thin film evaporation method. We have isolated the smallest size nanoliposomes after 90 min ultrasonification, keeping Polydisperse Index as 0.259. The morphology, size, zepta potential and drug efficiency of prepared nanoliposomes are characterized by using Transmission Electron Microscope (TEM), particle size analyzer and High Pressure Liquid Chromatography (HPLC). The particle size analyzer have confirmed the particle size of nanoluposomes measured in range of 100-250 nm, whereas the shape of these nanoliposomes is almost spherical. The zeta potential of small size nanoliposomes was measured as -49.62 and encapsulation efficiency of the LX loaded nanoliposomes was 87%. The oxidative stress response in SH-SY5Y Cells for various doses of drug with and without nanoliposomes has affectively improved the cell-stress response up to 20% after 24 h of incubation at 37 degrees C. The results indicated that LX loaded nanoliposomes were taken by the cells effectively which ultimately improved the cell-stress response. Thus, this study confirmed that synthesized nanoliposomes are not only effective drug carriers but could be potentially used for delivery of genes, antibodies, and proteins in future.

Synthesis, Characterization, Immune Regulation, and Antioxidative Assessment of Yeast-Derived Selenium Nanoparticles in Cyclophosphamide-Induced Rats

“…The antioxidant capacity is an important index reflecting the growth performance and health of animals. MDA is the main product of lipid peroxidation and its accumulation can reflect the degree of lipid peroxidation in animal organism, however, SOD, GSH-Px, and catalase activity are its good scavengers and therefore these indicators are often used to reflect the animal’s antioxidant status. , Se is a co-factor of the antioxidant enzyme GSH-Px, which is involved in scavenging free radicals and blocking the lipid peroxidation of cells, membranes, and organelles . However, the existence of Se ester bonds (Se–O–C) in the yeast-derived Se-NPs makes the physical and chemical properties of the compound change greatly, and it has a higher biological activity.…”

Fractionation of Biomolecules in Withania coagulans Extract for Bioreductive Nanoparticle Synthesis, Antifungal and Biofilm Activity

“…Figure 5 a shows bead-shaped Co 3 O 4 NPs obtained using methanol solvent extract as reducing agent [ 41 ] while in Figure 5 b the shape of Co 3 O 4 NPs obtained with hexane was different because of the different biomolecules present as compared to methanol solvent [ 42 ]. In the case of a mixed ratio of methanol and hexane solvents ( Figure 5 c), the Co 3 O 4 NPs were cube-shaped, most probably because of the action of different active biomolecules in this fraction when they reduce the cobalt nanoparticles [ 27 , 43 ].…”

“…The calculated values are listed in Table [42]. In the case of a mixed ratio of methanol and hexane solvents (Figure 5c), the Co 3 O 4 NPs were cube-shaped, most probably because of the action of different active biomolecules in this fraction when they reduce the cobalt nanoparticles [27,43]. The scheme ( Figure 1) shows a double dip strategy where the nature and concentration of a solvent reduce the complexity, provide active sites and finally highlight the functional activity of the biomolecules.…”

Cyclodextrin-Modified Nanomaterials for Drug Delivery: Classification and Advances in Controlled Release and Bioavailability

“…Liposomes are small artificial vesicles of spherical shape, consisting of one or more lipid bilayers, mainly composed of amphipathic phospholipids with a non-polar tail and polar head, enclosing an aqueous interior space [53,54]. Different strategies have been developed to combine liposomes and CDs, for example, the inclusion of complexes within the aqueous nucleus of different liposomes, a strategy called "drug-in CD-in liposome systems".…”