LuxA gene of light organ symbionts of the bioluminescent fish Acropoma japonicum (Acropomatidae) and Siphamia versicolor (Apogonidae) forms a lineage closely related to that of Photobacterium leiognathi ssp. mandapamensis

Wada, Mitsuru; Kamiya, Akio; Uchiyama, Nami; Yoshizawa, Susumu; Kita-Tsukamoto, Kumiko; Ikejima, Kou; Yu, Reiko; Imada, Chiaki; Karatani, Hajime; Mizuno, Naoki; Suzuki, Yuzuru; Nishida, Mutsumi; Kogure, Kazuhiro

doi:10.1111/j.1574-6968.2006.00322.x

Cited by 22 publications

(27 citation statements)

References 24 publications

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“…Photobacterium leiognathi has repeatedly been identified as the specific, exclusive light organ symbiont of leiognathid fishes (Perciformes, Leiognathidae), a well-studied group of marine bioluminescent fishes (e.g., see references 1, 3, 6, 9, 36, and 38), and P. mandapamensis has not previously been reported to be isolated from leiognathid light organs. Consistent with our hypothesis, a recent report indicates that bacteria provisionally identified as P. mandapamensis are light organ symbionts of two other fishes, Acropoma japonicum (Perciformes, Acropomatidae) and Siphamia versicolor (Perciformes, Apogonidae) (41).…”

supporting

confidence: 78%

“…4). The results clearly contradict the suggestion, based on limited data, that the symbionts of certain of these fishes have diverged in a host-dependent manner (41). Instead of identifying a matchup between host and symbiont phylogenies, this analysis indicates that P. mandapamensis may be more of a generalist as a light organ symbiont, as reflected by the phylogenetic diversity of its host fishes.…”

Section: Discussioncontrasting

confidence: 56%

“…Furthermore, for the symbionts of A. japonicum and S. versicolor, only phenotypic and limited sequence analysis had been carried out (14,16,19,22,33,41,44), leaving the identities of these bacteria in substantial doubt. In contrast, the extensive sampling and multigene phylogenetic analysis described here establishes P. mandapamensis as a light organ symbiont of these fishes, either as the apparently exclusive symbiont or as a cosymbiont with P. leiognathi.…”

Section: Discussionmentioning

confidence: 99%

“…Specifically, the light organ bacteria from A. japonicum, S. versicolor, and cer-tain other Siphamia species have been identified variously in the past either as P. leiognathi or as P. mandapamensis (12,14,16,19,22,33,41,44). Furthermore, except for leiognathid fishes, relatively few species of host animals have been sampled for luminous bacteria.…”

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confidence: 99%

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Phylogenetic Diversity and Cosymbiosis in the Bioluminescent Symbioses of “Photobacterium mandapamensis”

Kaeding

Ast

Pearce

et al. 2007

Appl Environ Microbiol

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"Photobacterium mandapamensis" (proposed name) and Photobacterium leiognathi are closely related, phenotypically similar marine bacteria that form bioluminescent symbioses with marine animals. Despite their similarity, however, these bacteria can be distinguished phylogenetically by sequence divergence of their luminescence genes, luxCDAB(F)E, by the presence (P. mandapamensis) or the absence (P. leiognathi) of luxF and, as shown here, by the sequence divergence of genes involved in the synthesis of riboflavin, ribBHA. To gain insight into the possibility that P. mandapamensis and P. leiognathi are ecologically distinct, we used these phylogenetic criteria to determine the incidence of P. mandapamensis as a bioluminescent symbiont of marine animals. Five fish species, Acropoma japonicum (Perciformes, Acropomatidae), Photopectoralis panayensis and Photopectoralis bindus (Perciformes, Leiognathidae), Siphamia versicolor (Perciformes, Apogonidae), and Gadella jordani (Gadiformes, Moridae), were found to harbor P. mandapamensis in their light organs. Specimens of A. japonicus, P. panayensis, and P. bindus harbored P. mandapamensis and P. leiognathi together as cosymbionts of the same light organ. Regardless of cosymbiosis, P. mandapamensis was the predominant symbiont of A. japonicum, and it was the apparently exclusive symbiont of S. versicolor and G. jordani. In contrast, P. leiognathi was found to be the predominant symbiont of P. panayensis and P. bindus, and it appears to be the exclusive symbiont of other leiognathid fishes and a loliginid squid. A phylogenetic test for cospeciation revealed no evidence of codivergence between P. mandapamensis and its host fishes, indicating that coevolution apparently is not the basis for this bacterium's host preferences. These results, which are the first report of bacterial cosymbiosis in fish light organs and the first demonstration that P. leiognathi is not the exclusive light organ symbiont of leiognathid fishes, demonstrate that the host species ranges of P. mandapamensis and P. leiognathi are substantially distinct. The host range difference underscores possible differences in the environmental distributions and physiologies of these two bacterial species.

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supporting

confidence: 78%

Section: Discussioncontrasting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Phylogenetic Diversity and Cosymbiosis in the Bioluminescent Symbioses of “Photobacterium mandapamensis”

Kaeding

Ast

Pearce

et al. 2007

Appl Environ Microbiol

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“…At present, the genus comprises 19 species and five species in this genus contain luminous strains: Photobacterium phosphoreum (Reichelt & Baumann, 1973) Thompson et al, 2007a;Wada et al, 2006).…”

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confidence: 99%

Photobacterium aquimaris sp. nov., a luminous marine bacterium isolated from seawater

Yoshizawa

Wada

Kita-Tsukamoto

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Two luminous marine bacteria, strains LC2-065T and LC2-102, were isolated from seawater at Sagami Bay in Japan. These bacteria were Gram-negative, oxidase-negative, catalase-positive, motile and coccoid-rods. 16S rRNA gene sequence analysis and multilocus sequence analysis (MLSA) using six loci (ftsZ, gapA, gyrB, mreB, pyrH and topA) and sequence analysis of the alpha subunit of luciferase (luxA) gene revealed that these bacteria were distinct from other species of the genus Photobacterium. These novel strains were most closely related to Photobacterium kishitanii. The DNA-DNA hybridization value between strain LC2-065 T and Photobacterium kishitanii ATCC BAA-1194 T was 42.1 %. The major fatty acids were C 12 : 0, C 14 : 0 , C 16 : 0 , C 18 : 0 and C 15 : 0 iso 2-OH and/or C 16 : 1 v7c (summed feature 3). The DNA G+C contents of strains LC2-065 T and LC2-086 were 42.2 and 42.9 mol%, respectively. The phenotypic features of the novel strains were similar to those of P. kishitanii and P. phosphoreum, but there were sufficient physiological differences for the novel strains to be easily differentiated. On the basis of these results, these new strains represent a novel species, for which the name Photobacterium aquimaris sp. nov. is proposed. The type strain is LC2-065 T (5NBRC 104633The genus Photobacterium was first described by Beijerinck (1889). At present, the genus comprises 19 species and five species in this genus contain luminous strains: Photobacterium phosphoreum (Reichelt & Baumann, 1973) Thompson et al., 2007a;Wada et al., 2006).In this study, two luminescent strains were isolated from seawater and analysed using 16S rRNA, the alpha-subunit of luciferase (luxA), uridylate kinase (pyrH), a cell division protein (ftsZ), a rod shaping protein (mreB), the DNA gyrase B-subunit (gyrB), topoisomerase I (topA) and glyceraldehyde 3-phosphate dehydrogenase (gapA) gene sequences. Based on the phylogenetic analyses, the novel strains formed a tight cluster in which no recognized type species of the genus Photobacterium was grouped. This suggests that the new isolates represent a novel species of the genus Photobacterium. Therefore, we conducted a series of analyses to fully identify these isolates. Based on the results of phylogenetic and physiological and analyses, these strains are proposed to represent a novel species. T and LC2-086 were isolated from samples collected at a depth of 50 m, 15.6 uC, and at 1000 m, 3.2 uC, respectively. The seawater samples were filtered through a polycarbonate filter (pore size 0.2 mm; Whatman International Ltd) and then the filter was placed on half strength marine agar 2216E (MA; Difco) and maintained at 20 u C. Luminous colonies that grew on the agar plates were isolated withThe GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, ftsZ, gapA, gyrB, mreB, pyrH and topA gene sequences used and determined in this study are given in Supplementary Table S1, available with the online version of this paper.Supplementary tables detailing the GenBank accession numbers for the 16S rRNA,...

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Photobacterium

Labella

Borrego

2023

Bergey's Manual of Systematics of Archaea and Bacteria

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Pho.to.bac.te'ri.um. Gr. neut. n. phôs , light; Gr. neut. dim. n. bakterion , a small rod; N.L. neut. n. Photobacterium , light (‐producing) bacterium. Proteobacteria / Gammaproteobacteria / Vibrionales / Vibrionaceae / Photobacterium The genus Photobacterium contains 37 species with validated names and 3 species whose names have not been validated. Cells are short bacilli Gram‐stain negative, and nonsporulated. Facultatively anaerobic. Require NaCl for growth (optimal concentration 1–3%). Several species present bioluminescence. Some of them are piezophilic and others are psychrophilic. Isolated from marine environment (seawater and sediment), associated with aquatic animals (mutualism relationships), but some are pathogens for poikilothermic and homeothermic animals. DNA G + C content (mol%) : 38.5–53.6. Type species : Photobacterium phosphoreum Beijerinck 1889 AL (basonym: Micrococcus phosphoreus Cohn 1878).

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LuxA gene of light organ symbionts of the bioluminescent fish Acropoma japonicum (Acropomatidae) and Siphamia versicolor (Apogonidae) forms a lineage closely related to that of Photobacterium leiognathi ssp. mandapamensis

Cited by 22 publications

References 24 publications

Phylogenetic Diversity and Cosymbiosis in the Bioluminescent Symbioses of “Photobacterium mandapamensis”

Phylogenetic Diversity and Cosymbiosis in the Bioluminescent Symbioses of “Photobacterium mandapamensis”

Photobacterium aquimaris sp. nov., a luminous marine bacterium isolated from seawater

Photobacterium

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