Lupane Glycosides from the Leaves of Acanthopanax koreanum

Choi, Hyun-Sik; Kim, Hyoung Ja; Nam, Sang-Gil; Kim, In-Seon; Lee, Kyung‐Tae; Yook, Chang‐Soo; Lee, Yong Sup

doi:10.1248/cpb.56.1613

Cited by 25 publications

(12 citation statements)

References 17 publications

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“…Bioactive constituents of A. koreanum have been reported including several lignans, diterpenes [20,21], and lupane-type triterpene glycosides, which are considered as major constituents [22,23]. Since we have established the combination of ELISA and eastern blotting methods using anti-ginsenoside Rb 1 MAb for the identification of ginsenoside Rb 1 in Panax species and traditional Chinese medicines [14] as a high sensitive and rapid method, the finding and determination of ginsenoside Rb 1 in A. koreanum of Araliaceae family by using ELISA and eastern blotting monitoring will be reviewed in this section.…”

Section: Eastern Blotting Fingerprint For Ginsenosidesmentioning

confidence: 99%

Determination and Isolation of Ginsenosides from Araliaceous Plants by using Eastern Blotting Fingerprinting

Tung¹,

Fukuda²,

Tanaka³

et al. 2014

Nat Prod Chem Res 2014

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In the eastern blotting method, a new fashion to separate the ginsenoside molecule into two functional parts using a simple and well-known chemical reaction was developed. In principle, the sugar moieties were oxidized by NaIO 4 to form dialdehydes, which reacted with amino groups of the protein and covalently bound to the adsorbent PVDF membrane. The MAb bound to the aglycon part of the ginsenoside molecule for immunostaining. Double staining of eastern blotting for ginsenosides using anti-ginsenoside-Rb 1 and-Rg 1 MAbs promoted complete identification of ginsenosides in Panax species. As an application, we analyzed several Araliaceous plants by eastern blotting and enzyme-linked immunosorbent assay (ELISA) using anti-ginsenoside Rb 1 MAb leading to the investigation of ginsenoside Rb 1 from Kalopanax pictus and Acanthopanax koreanum. On the other hand, by immunoassay-guided fractionation and chromatography separation on the methanol extract of American ginseng, two new minor ginsenosides were isolated. As another application, identification of two known ginsenosides was achieved from the P. japonicas extract using eastern blotting and immunoaffinity column combined with anti-ginsenoside Rb 1 MAb.

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Section: Eastern Blotting Fingerprint For Ginsenosidesmentioning

confidence: 99%

Determination and Isolation of Ginsenosides from Araliaceous Plants by using Eastern Blotting Fingerprinting

Tung¹,

Fukuda²,

Tanaka³

et al. 2014

Nat Prod Chem Res 2014

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“…Phytochemical profile of A. koreanum have been documented with several lignans and diterpenes [3,4] along with, especially, various lupane-type triterpene saponins, which are considered as major constituents [5,6].…”

Section: Introductionmentioning

confidence: 99%

Investigation of Ginsenoside Rb1 from Acanthopanax koreanum by Eastern Blotting and ELISA Analyses

Tung¹,

Uto²

2016

Pharm Anal Chem

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Ginsenosides such as ginsenoside Rb 1 are the principal components in Panax spp. and have been found in some other Araliaceae plants. In our survey of ginsenoside from Araliaceous species based on eastern blotting and ELISA methods using antiginsenoside Rb 1 monoclonal antibody, ginsenoside Rb 1 , one of the principal ginseng saponins, was investigated with concentrations of 0.000016, 0.000014, and 0.000039% dry weight in the leaves, stem, and roots of the well-known medicinal plant Acanthopanax koreanum respectively. The obtained results further support potential and promising application of MAb including eastern blotting and ELISA for surveying ginsenoside sources including ginsenoside Rb 1 .

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“…Previous phytochemical and biological investigations of A. koreanum have resulted in the identification of a number of triterpenes (Chang et al, 1998(Chang et al, , 1999Cai et al, 2004;Park et al, 2005;Choi et al, 2008) and some of the terpenoids have been shown to have anti-inflammatory properties. For example, impressic acid, a triterpenoid isolated from the root of A. koreanum, inhibited NFAT transcription factor activity (Cai et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory activity on LPS-stimulated dendritic cells of lupanetype triterpenoids from the leaves of Acanthopanax koreanum

et al. 2011

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Acanthopanax koreanum is well known herb in traditional Korean, Chinese, and Japanese anti-inflammatory action without any adverse effects. In the current study, we investigated the inhibitory effects of isolated compounds 1-13 from the leaves of A. koreanum on the lipopolysaccharide-stimulated production of pro-inflammatory cytokines in bone marrow-derived dendritic cells. Of these lupane-type triterpenoids, 1 exhibited particularly high inhibitory effect on lipopolysaccharide-stimulated TNF-α, IL-6, and IL-12 production with the values ranging from 45.0 to 84.5% at a concentration of 50 μM. These results warrant further studies concerning the potential anti-inflammatory benefits of medicinal foods containing the leaves of A. koreanum.

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Lupane Glycosides from the Leaves of Acanthopanax koreanum

Cited by 25 publications

References 17 publications

Determination and Isolation of Ginsenosides from Araliaceous Plants by using Eastern Blotting Fingerprinting

Determination and Isolation of Ginsenosides from Araliaceous Plants by using Eastern Blotting Fingerprinting

Investigation of Ginsenoside Rb1 from Acanthopanax koreanum by Eastern Blotting and ELISA Analyses

Anti-inflammatory activity on LPS-stimulated dendritic cells of lupanetype triterpenoids from the leaves of Acanthopanax koreanum

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