Lucidumol C, a new cytotoxic lanostanoid triterpene from Ganoderma lingzhi against human cancer cells

Amen, Yhiya; Zhu, Qing; Tran, Hai Bang; Afifi, Mohamed; Halim, Ahmed F.; Ashour, Ahmed; Mira, Amira; Shimizu, Kuniyoshi

doi:10.1007/s11418-016-0976-2

Cited by 27 publications

(7 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ganoderma triterpenes have been reported to be cytotoxic to several cancer cell lines in vitro and to inhibit tumor invasion in vitro and in vivo [4,5,6,27,28,29,44,45,46,47]. Currently, most of the triterpenes and TAs with reported antitumor activities are isolated from the fruiting bodies or spores of Ganoderma (mainly G. lucidum ) [4,6,27,28,29,47].…”

Section: Discussionmentioning

confidence: 99%

Improved Production and Antitumor Properties of Triterpene Acids from Submerged Culture of Ganoderma lingzhi

et al. 2016

View full text Add to dashboard Cite

Triterpene acids (TAs) are the major bioactive constituents in the medicinal fungus Ganoderma lingzhi. However, fermentative production of TAs has not been optimized for commercial use, and whether the TAs isolated from G. lingzhi submerged culture mycelia possess antitumor activity needs to be further proven. In this study, enhanced TA yield and productivity were attained with G. lingzhi using response surface methodology. The interactions of three variables were studied using a Box-Benhnken design, namely initial pH, dissolved oxygen (DO) and fermentation temperature. The optimum conditions were an initial pH of 5.9, 20.0% DO and 28.6 ○ C. These conditions resulted in a TA yield of 308.1 mg/L in a 5-L stirred bioreactor. Furthermore, the optimized conditions were then successfully scaled up to a production scale of 200 L, and maximum TA production and productivity of 295.3 mg/L and 49.2 mg/L/day were achieved, which represented 80.9% and 111.5% increases, respectively, compared with the non-optimized conditions. Additionally, the triterpene acid extract (TAE) from G. lingzhi mycelia was found to be cytotoxic to the SMMC-7721 and SW620 cell lines in vitro, and the TAE exhibited dose-dependent antitumor activity against the solid tumor sarcoma 180 in vivo. Chemical analysis revealed that the key active triterpene compounds, ganoderic acid T and ganoderic acid Me, predominated in the extract.

show abstract

Section: Discussionmentioning

confidence: 99%

Improved Production and Antitumor Properties of Triterpene Acids from Submerged Culture of Ganoderma lingzhi

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Furthermore, the hydroxy groups covalently connected to a carbon atom in triterpenes are frequently oxidized, likely enabling the triterpenoids to have such antioxidant pathways. Indeed, Lucidumol C and D have been reported to be antioxidant molecules that consequently suppress cancer progression [ 26 , 27 ]. Moreover, our data suggest that Lucidumol A suppressed the expression of anti-inflammatory molecules such as COX-2 and iNOS (Figures 3 – 5 ).…”

Section: Discussionmentioning

confidence: 99%

Lucidumol A, Purified Directly from Ganoderma lucidum, Exhibits Anticancer Effect and Cellular Inflammatory Response in Colorectal Cancer

Shin

Chae

Lee

et al. 2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Colorectal cancer (CRC) is a deadly disease regardless of sex, and a few therapeutic approaches have been fully developed at advanced stages, even if some strategies have durable clinical benefits, such as immunotherapy and chemotherapy. Ganoderma lucidum has been recognized as an organism that suppresses tumors and inflammation; however, the molecular mechanisms induced by a triterpenoid in Ganoderma lucidum, Lucidumol A, have not yet been fully explored in CRC and inflammatory responses. To this end, we extracted Lucidumol A from Ganoderma lucidum and analyzed its anticancer effect and anti-inflammatory potential in CRC cell lines and RAW264.7 macrophage-derived cell lines, respectively. A series of in vitro experiments including cell survival, wound healing, and migration assays were performed to determine the role of Lucidumol A in the CRC cell line. We also analyzed inflammatory responses using qRT-PCR, Western Blot, and ELISA in RAW 264.7 macrophaged-derived cell lines exposed to various concentrations of Lucidumol A. Lucidumol A efficiently suppressed the metastatic potential of CRC at very low concentrations. Furthermore, significant anti-inflammatory activities were observed in Lucidumol A-treated RAW264.7 cells through modulation of inflammation-associated marker genes and cytokines. In conclusion, Lucidumol A plays an important role in Ganoderma lucidum-dependent tumor suppression and anti-inflammation, suggesting different strategies to treat CRC patients, and other diseases evoked by proinflammatory cytokines, despite the need to explore further its mechanism of action.

show abstract

“…Ganoderma lingzhi , a closely related strain to G. lucidum (Leyss.:Fr.) Karst, was the vegetal source of lucidumol C, an oxygenated lanostane derivative which was assessed against several cancer cell lines, including HeLa cells, and revealed remarkable cytotoxic properties and high selectivity on cancer cells ( 162 ). By comparing lucidumol C to its non-cytotoxic analogue, lucidumol A, the authors hypothesized that its cytotoxic activity is presumably related to the presence of a C-11 carbonyl group in its structure.…”

Section: Triterpenes As Therapeutic Agents In Sex Hormone-dependent Cmentioning

confidence: 99%

Natural Compounds in Sex Hormone-Dependent Cancers: The Role of Triterpenes as Therapeutic Agents

et al. 2021

View full text Add to dashboard Cite

Sex hormone-dependent cancers currently contribute to the high number of cancer-related deaths worldwide. The study and elucidation of the molecular mechanisms underlying the progression of these tumors was a double-edged sword, leading to the expansion and development of new treatment options, with the cost of triggering more aggressive, therapy resistant relapses. The interaction of androgen, estrogen and progesterone hormones with specific receptors (AR, ER, PR) has emerged as a key player in the development and progression of breast, ovarian, prostate and endometrium cancers. Sex hormone-dependent cancers share a common and rather unique carcinogenesis mechanism involving the active role of endogenous and exogenous sex hormones to maintain high mitotic rates and increased cell proliferation thus increasing the probability of aberrant gene occurrence and accumulation highly correlated with abnormal cell division and the occurrence of malignant phenotypes. Cancer related hormone therapy has evolved, currently being associated with the blockade of other signaling pathways often associated with carcinogenesis and tumor progression in cancers, with promising results. However, despite the established developments, there are still several shortcomings to be addressed. Triterpenes are natural occurring secondary metabolites biosynthesized by various pathways starting from squalene cyclization. Due to their versatile therapeutic potential, including the extensively researched antiproliferative effect, these compounds are most definitely a cornerstone in the research and development of new natural/semisynthetic anticancer therapies. The present work thoroughly describes the ongoing research related to the antitumor activity of triterpenes in sex hormone-dependent cancers. Also, the current review highlights both the biological activity of various triterpenoid compounds and their featured mechanisms of action correlated with important chemical structural features.

show abstract

Lucidumol C, a new cytotoxic lanostanoid triterpene from Ganoderma lingzhi against human cancer cells

Cited by 27 publications

References 19 publications

Improved Production and Antitumor Properties of Triterpene Acids from Submerged Culture of Ganoderma lingzhi

Improved Production and Antitumor Properties of Triterpene Acids from Submerged Culture of Ganoderma lingzhi

Lucidumol A, Purified Directly from Ganoderma lucidum, Exhibits Anticancer Effect and Cellular Inflammatory Response in Colorectal Cancer

Natural Compounds in Sex Hormone-Dependent Cancers: The Role of Triterpenes as Therapeutic Agents

Contact Info

Product

Resources

About