&lt;p&gt;The Antibacterial Activity and Mechanism of Action of Luteolin Against &lt;em&gt;Trueperella pyogenes&lt;/em&gt;&lt;/p&gt;

Guo, Yongfeng; Liu, Yan; Zhang, Zehui; Chen, Menghan; Zhang, Dexian; Tian, Chunlian; Liu, Mingchun; Guotuo, Jiang

doi:10.2147/idr.s253363

Cited by 69 publications

(48 citation statements)

References 62 publications

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“…After being treated by SAEW for 15, 30, 45, and 60 s, the NPN fluorescence intensity of P. deceptionensis CM2 cells was increased by 202%, 255%, 266%, and 271%, respectively, compared to the untreated cells ( p < 0.05). These data indicate that SAEW disrupted the extracellular membranes of P. deceptionensis CM2 cells, which might contribute to the cell death [ 34 ]. Previous studies showed that AEW with high ORP caused the oxidation of sulfhydryl groups on cell surfaces and disturbed metabolic pathways inside the bacterial cells, which might be responsible for cell death [ 35 , 36 ].…”

Section: Resultsmentioning

confidence: 99%

Inactivation and Membrane Damage Mechanism of Slightly Acidic Electrolyzed Water on Pseudomonas deceptionensis CM2

et al. 2021

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Pseudomonas is considered as the specific spoilage bacteria in meat and meat products. The purpose of this study was to evaluate the inactivation efficiency and mechanisms of slightly acidic electrolyzed water (SAEW) against Pseudomonas deceptionensis CM2, a strain isolated from spoiling chicken breast. SAEW caused time-dependent inactivation of P. deceptionensis CM2 cells. After exposure to SAEW (pH 5.9, oxidation–reduction potential of 945 mV, and 64 mg/L of available chlorine concentration) for 60 s, the bacterial populations were reduced by 5.14 log reduction from the initial load of 10.2 log10 CFU/mL. Morphological changes in P. deceptionensis CM2 cells were clearly observed through field emission-scanning electron microscopy as a consequence of SAEW treatment. SAEW treatment also resulted in significant increases in the extracellular proteins and nucleic acids, and the fluorescence intensities of propidium iodide and n-phenyl-1-napthylamine in P. deceptionensis CM2 cells, suggesting the disruption of cytoplasmic and outer membrane integrity. These findings show that SAEW is a promising antimicrobial agent.

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Section: Resultsmentioning

confidence: 99%

Inactivation and Membrane Damage Mechanism of Slightly Acidic Electrolyzed Water on Pseudomonas deceptionensis CM2

et al. 2021

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“…The enhancement in the activity may be due to the higher solubility of LT in the presence of the used edge activator as well as the nano size of vesicles. Due to the higher solubility of LT, it showed activity by destroying the cell wall and cell membrane and inhibiting nucleic acid synthesis [54].…”

Section: Antibacterial Activitymentioning

confidence: 99%

Formulation, Optimization and Evaluation of Luteolin-Loaded Topical Nanoparticulate Delivery System for the Skin Cancer

et al. 2021

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In the present study, luteolin (LT)-loaded nanosized vesicles (LT-NVs) were prepared by a solvent evaporation–hydration method using phospholipid and edge activator. The formulation was optimized using three factors at a three-level Box–Behnken design. The formulated LT-NVs were prepared using the three independent variables phospholipid (A), edge activator (B) and sonication time (C). The effect of used variables was assessed on the vesicle size (Y1) and encapsulation efficiency (Y2). The selection of optimum composition (LT-NVopt) was based on the point prediction method of the software. The prepared LT-NVopt showed the particle size of 189.92 ± 3.25 nm with an encapsulation efficiency of 92.43 ± 4.12% with PDI and zeta potential value of 0.32 and −21 mV, respectively. The formulation LT-NVopt was further converted into Carbopol 934 gel (1% w/v) to enhance skin retention. LT-NVoptG was further characterized for viscosity, spreadability, drug content, drug release, drug permeation and antioxidant, antimicrobial and cytotoxicity assessment. The evaluation result revealed optimum pH, viscosity, spreadability and good drug content. There was enhanced LT release (60.81 ± 2.87%), as well as LT permeation (128.21 ± 3.56 µg/cm2/h), which was found in comparison to the pure LT. The antioxidant and antimicrobial study results revealed significantly (p ˂ 0.05) better antioxidant potential and antimicrobial activity against the tested organisms. Finally, the samples were evaluated for cytotoxicity assessment using skin cancer cell line and results revealed a significant difference in the viability % at the tested concentration. LT-NVoptG showed a significantly lower IC50 value than the pure LT. From the study, it can be concluded that the prepared LT-NVoptG was found to be an alternative to the synthetic drug as well as conventional delivery systems.

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“…The main strategies by which luteolin affects bacterial proteins include binding to or interacting with proteins, decreasing the secretion of proteins, and inhibiting protein expression [29][30][31][32]. Our preliminary data suggest that luteolin can affect the expression of proteins and interfere with the normal processes of T. pyogenes [33]. Interestingly, luteolin also exerted a significant inhibitory effect on the production of the MATE protein in T. pyogenes by downregulating the expression of the MATE gene [34].…”

Section: Introductionmentioning

confidence: 75%

“…The T. pyogenes isolate BMH06-3 was collected from dairy cattle in Liaoning, China, and identified by 16S rRNA gene sequencing [33]. T. pyogenes strains were cultured on Mueller-Hinton agar (MHA, Solarbio, Beijing, China) containing 5% (v/v) sheep blood (Solarbio, Beijing, China) in an incubator (5% CO2) at 37 °C for 36 h, and the colonies were inoculated in nutrient broth (NB, Solarbio, Beijing, China) supplemented with 8% (v/v) fetal bovine serum (FBS, Gibco, Grand Island, USA).…”

Section: T Pyogenes Strains and Culturementioning

confidence: 99%

“…Our previous research showed that the minimum inhibitory concentration (MICs) of luteolin against isolates of T. pyogenes is 78 μg/mL [33]. TatD DNases (2 μM) and different MICs (final concentrations: 0, 1/8, 1/4, 1/2, 1, 2 and 4 MIC) of luteolin were added to Eppendorf tubes and supplemented with PBS (pH=7.4) to 20 μL.…”

Section: Gel Assay Of Luteolin-tptatd Interactionsmentioning

confidence: 99%

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A Functional Study of the TatD DNases of <em>Trueperella pyogenes</em> and the Conformational Analysis of Luteolin as an Inhibitor

Zhang¹,

Liang²,

Yu³

et al. 2021

Preprint

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Trueperella pyogenes (T. pyogenes) can cause a variety of infections in animals and lead to economic loss in animal husbandry. TatD DNases are considered to be potential virulence factors in Plasmodium falciparum and Streptococcus pneumoniae. However, the function of TatD DNases in T. pyogenes is still unclear. Therefore, the aim of this study was to illustrate the function of TatD DNases of T. pyogenes (TpTatDs) and investigate whether luteolin is able to inhibit DNase function. The findings of our study are anticipated to be crucial to treating infections caused by T. pyogenes. Bioinformatic analysis has been used for the prediction of crucial functional residues of TpTatDs. The function of TpTatDs was investigated in the presence of divalent cations by hydrolyzing DNA with recombinant TatD proteins. Luteolin is a candidate nuclease inhibitor evaluated in our study. The interactions between luteolin and TpTatDs were tested using molecular docking analysis and surface plasmon resonance (SPR) assays. The inhibitory effect of luteolin on TpTatDs was analyzed by agarose gel electrophoresis. Two genes in the genome of T. pyogenes are suspected to encode TatD DNases. According to the length of their nucleotide sequences, they were named tatD960 and tatD825. Both of the TpTatDs, which are magnesium-dependent, were able to hydrolyze linear DNA and plasmids. In this study, we found through molecular docking analysis and SPR assays that luteolin can stably bind with TpTatDs. The gel assay revealed that luteolin can inhibit the DNase activity of TpTatDs. Our results indicated that TatD DNases from T. pyogenes are Mg2+-dependent DNases and exhibit DNA endonuclease activity. Moreover, luteolin reduced their DNA hydrolysis ability by decreasing the binding between TpTatDs and DNA.

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<p>The Antibacterial Activity and Mechanism of Action of Luteolin Against <em>Trueperella pyogenes</em></p>

Cited by 69 publications

References 62 publications

Inactivation and Membrane Damage Mechanism of Slightly Acidic Electrolyzed Water on Pseudomonas deceptionensis CM2

Inactivation and Membrane Damage Mechanism of Slightly Acidic Electrolyzed Water on Pseudomonas deceptionensis CM2

Formulation, Optimization and Evaluation of Luteolin-Loaded Topical Nanoparticulate Delivery System for the Skin Cancer

A Functional Study of the TatD DNases of <em>Trueperella pyogenes</em> and the Conformational Analysis of Luteolin as an Inhibitor

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