<p>Poly(N-Isopropyl-Acrylamide)/Poly(γ-Glutamic Acid) Thermo-Sensitive Hydrogels Loaded with Superoxide Dismutase for Wound Dressing Application</p>

Abstract

Introduction: Chronic trauma repair is an important issue affecting people's healthy lives. Thermo-sensitive hydrogel is injectable in situ and can be used to treat large-area wounds. In addition, antioxidants play important roles in promoting wound repair. Methods: The purpose of this research was to prepare a novel thermo-sensitive hydrogelpoly(N-isopropyl-acrylamide)/poly(γ-glutamic acid) (PP) loaded with superoxide dismutase (SOD) to improve the effect for trauma treatment. The micromorphology of the hydrogel was observed by scanning electron microscope and the physical properties were measured. The biocompatibility of hydrogel was evaluated by MTT experiment, and the effect of hydrogel on skin wound healing was evaluated by in vivo histological staining. Results: Gelling behavior and differential scanning calorimeter outcomes showed that the PP hydrogels possessed thermo-sensitivity at physiological temperature and the phase transformation temperature was 28.2°C. The high swelling rate and good water retention were conducive to wound healing. The activity of SOD in vitro was up to 85% at 10 h, which was advantageous to eliminate the superoxide anion. MTT assay revealed that this hydrogel possessed good biocompatibility. Dressings of PP loaded with SOD (SOD-PP) had a higher wound closure rate than other treatments in vivo in diabetic rat model. Discussion: The SOD-PP thermo-sensitive hydrogels can effectively promote wound healing and have good application prospects for wound repair.

LPS-stimulated Macrophage Exosomes Inhibit Inflammation by Activating the Nrf2&nbsp;/ HO-1 Defense Pathway and Promote Wound Healing in Diabetic Rats

“…Reactive oxygen species (ROS) are produced in large quantities in skin wounds due to chronic in ammation, which will further aggravate the in ammatory response and toxic effects of cells [43].The reduction of antioxidant active substances such as superoxide dismutase (SOD) and glutathione peroxidase (GPx) and the increase of free radicals can all lead to delayed healing of diabetic wounds [44].This is consistent with our experimental results. Through extracorporeal experiments, we can nd that LPS-Exos can signi cantly reduce the reactive oxygen species induced by endothelial cells under high glucose treatment.At the same time, different concentrations of LPS-Exos can increase the antioxidant substances in the wounds of diabetic rats to different degrees.…”

Fabrication and Characterization of Glycerol/Chitosan/Polyvinyl Alcohol-Based Transparent Hydrogel Films Loaded with Silver Nanoparticles for Antibacterial Wound Dressing Applications

“…Further, due to the hydrophilicity of the dressing used in the current study, it is easier to use than other dressings and is less painful to change. [ 3 25 26 ].…”

Superoxide Dismutase Administration: A Review of Proposed Human Uses

“…(2020). This formulation showed good biocompatibility and a wound closure rate after 21 days of operation, of up to 92% in diabetic rats [ 213 ]. Furthermore, SOD2 stimulated wound healing in streptozotocin-induced type I diabetes rats [ 209 ].…”