<p>MiR-29a function as tumor suppressor in cervical cancer by targeting SIRT1 and predict patient prognosis</p>

Abstract

IntroductionCervical cancer is the second most frequently malignant tumors in females and metastasis is a challenge of the treatment of cervical cancer. MiR-29a is usually low expressed in several tumors and its functions in cervical cancer remain unclear.Patients and methodsThe quantitative real-time polymerase chain reaction was employed to assess the expression of miR-29a and the Sirtuin-1 (SIRT1). Cell metastatic ability was assessed using Transwell and Western blot assays. The dual-luciferase reporter assay was performed to verify that miR-29a targeted to the 3’-untranslated region (UTR) of SIRT1 mRNA.ResultsMiR-29a was low expressed in cervical cancer and downregulation of miR-29a was associated with poor outcome. MiR-29a regulated the expression of SIRT1 by targeting to its 3’-UTR of mRNA in HeLa cells. SIRT1 was upregulated in cervical cancer tissues and cells in comparison with the non-tumor tissues and normal cells. Upregulation of SIRT1 predicted worse outcome of cervical cancer patients. MiR-29a was participated in the migration, invasion and epithelial–mesenchymal transition (EMT) in cervical cancer through directly targeting to the 3’-UTR of SIRT1 mRNA. SIRT1 reversed partial roles of miR-29a on metastasis in cervical cancer.ConclusionmiR-29a suppressed migration, invasion and EMT by directly targeting to SIRT1 in cervical cancer. The newly identified miR-29a/SIRT1 axis provides novel insight into the pathogenesis of cervical cancer.

miR-29a Modulates GSK3β/SIRT1-Linked Mitochondrial Proteostatic Stress to Ameliorate Mouse Non-Alcoholic Steatohepatitis

“…In this study, PPI analysis in silico shows that physical interaction is the primary relationship between GSK3β and SIRT1. In contrast, Nan et al has demonstrated that SIRT1 expression level can be regulated by direct binding of miR-29a on its 3 UTR in cervical cancer cells [43]. As such, whether SIRT1 expression targeted by miR-29a underpins the molecular mechanism in hepatocytes and liver of NASH model requires further study for clarification.…”

miR-29a-3p directly targets Smad nuclear interacting protein 1 and inhibits the migration and proliferation of cervical cancer HeLa cells

“…In contrast, other studies have shown that miR-29a was down-regulated and inhibited the progression of cancers such as colon cancer ( Shi et al, 2020 ), non-small cell lung cancer ( Hu et al, 2016 ) and adenocarcinoma ( Zhang et al, 2018 ). Previous studies have shown that miR-29a was low expression in cervical cancer, as a tumor suppressor, miR-29a can target multiple genes, such as CDC42, HSP47, SIRT1 and DNMT1 ( Park et al, 2009 ; Yamamoto et al, 2013 ; Gong et al, 2019 ; Nan et al, 2019 ). Nonetheless, the detailed biological function of miR-29a in cervical cancer has not yet been completely revealed.…”

Increased miR-20b Level in High Grade Cervical Intraepithelial Neoplasia

“…In association with CIN2-3 and cervical carcinoma, several further miRNAs showing dysregulated expression have been reported, such as miR-218, 29a, −101, −140-5p [40][41][42][43][44]. In the study of Gocze et al (2015), progressively increased expression of miR-27a was demonstrated in CIN2-3 as compared to CIN1 and in squamous cell carcinoma (SCC) as compared to CIN2-3, while the levels of miR-34a were found to be lower in CIN2-3 when compared to CIN1 and in SCC in comparison to CIN2-3.…”