&lt;p&gt;MiR-195-5p Inhibits Malignant Progression of Cervical Cancer by Targeting YAP1&lt;/p&gt;

Liu, Xiaomin; Zhou, Yi; Ning, Yuping; Gu, Hui; Tong, Yuxin; Wang, Ning

doi:10.2147/ott.s227826

Cited by 50 publications

(36 citation statements)

References 40 publications

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“…MicroRNAs (miRNAs) are implicated in tumorigenesis by binding to the 3' UTR of target mRNA and repressing their translation. Diverse miRNAs can participate in CC development as tumor-suppressive miRNAs or oncomiRs [6][7][8][9]. For instance, miR-145-5p targets fascin to impede cancer cell migration and invasion in CC [6].…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circ_0000228 promotes the malignancy of cervical cancer via microRNA-195-5p/ lysyl oxidase-like protein 2 axis

Liu

et al. 2021

Bioengineered

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Circular RNAs (circRNAs) are a class of novel non-coding RNAs that are vital in modulating gene expression and biological processes. Nevertheless, in cervical cancer (CC), the role of circRNA is much less investigated. In this work, circ_0000228 expression in CC is measured and circ_0000228's function and related mechanism are investigated. Quantitative real-time quantitative polymerase chain reaction (qRT-PCR) was utilized to examine the expression levels of circ_0000228, ) and lysyl oxidase-like protein 2 (LOXL2). Western blotting was employed to examine LOXL2 protein expression in CC cell lines. CC cell lines with circ_0000228 knockdown were constructed, and the CCK-8 experiment and Transwell experiment were executed to investigate the effect of circ_0000228 on the malignant characteristics of CC cells. Furthermore, a dual-luciferase reporter gene experiment was applied to validate the targeting relationship between circ_0000228 and In this study, we demonstrated that circ_0000228 showed a remarkable up-modulation in CC tissues and cell lines. Circ_0000228 knockdown repressed the growth and metastatic potential of CC cells. Mechanistically, circ_0000228 facilitated CC progression through sponging miR-195-5p and upmodulating LOXL2 expression. We conclude that circ_0000228 is an oncogenic circRNA, which participates in promoting CC progression via regulating the miR-195-5p/LOXL2 axis.

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Section: Introductionmentioning

confidence: 99%

Circular RNA circ_0000228 promotes the malignancy of cervical cancer via microRNA-195-5p/ lysyl oxidase-like protein 2 axis

Liu

et al. 2021

Bioengineered

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“…Previous studies, including own research, have confirmed that dysregulation of YAP1 results in tumorigenesis, including in TNBC ( 18 – 21 ). Several miRNA molecules, such as miR-195-5p ( 22 ), miR-630 ( 23 ) and miR-1285-3p ( 24 ) were identified as YAP1 regulators. Nevertheless, further understanding of the post-transcriptional control of YAP1 in TNBC is necessary.…”

Section: Introductionmentioning

confidence: 99%

miR‑497 inhibits proliferation and invasion in triple‑negative breast cancer cells via YAP1

Hua

Jin

et al. 2021

Oncol Lett

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MicroRNA (miR)-497 has been reported as a tumor suppressor in various cancer types. Nonetheless, the regulation of triple-negative breast cancer (TNBC) by miR-497 remains poorly understood. The present study aimed to investigate the potential function and mechanism of miR-497 in TNBC. A total of 36 TNBC and matched non-cancerous tissue samples were collected for analysis. Reverse transcription-quantitative PCR was performed to detect the miR-497 levels in TNBC tissue. The association between miR-497 expression, clinical characteristics and survival was then analyzed. To investigate the role of miR-497 in TNBC, MTT, colony formation, Transwell invasion, cell cycle and cell apoptosis assays were conducted following transfection of miR-497 mimics into the MDA-MB-231 and MDA-MB-468 cell lines. Luciferase reporter assays and western blot analysis were used to confirm the regulation of a putative target of miR-497. The results indicated that the expression of miR-497 was downregulated in the TNBC specimens. Further analysis demonstrated that the expression of miR-497 was downregulated in patients with advanced TNBC stages and that low miR-497 was associated with poor prognosis in patients with TNBC. Transfection of miR-497 mimics inhibited TNBC cell proliferation and increased cell apoptosis in MDA-MB-231 and MDA-MB-468 cells. Moreover, cell migration was inhibited following overexpression of miR-497, which also led to the arrest of the breast cancer cells in the G 0 /G 1 phase of the cell cycle. Yes-associated protein 1 (YAP1), a critical molecule in the Hippo pathway, was identified as a target of miR-497. Notably, the protein and mRNA expression levels of YAP1 in MDA-MB-231 and MDA-MB-468 cells were downregulated following overexpression of miR-497. Overall, the findings of the present study indicated that miR-497 inhibited TNBC cell proliferation and migration and induced cell apoptosis by negatively regulating YAP1 expression. Thus, targeting miR-497 may represent a potential strategy for the treatment of TNBC.

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“…indicating that miR-1258 can be used as a therapeutic target for human CRC (17). Another study showed that miR-195-5p can downregulate the expression of YAP1 and miR-195-5p-mediated downregulation of YAP1, which impedes genesis and development of tumors in CRC (18). For miR-672-3p, it has been noted that miR-672-3p can serve a role of tumor suppressor in a number of types of cancer (19).…”

Section: Discussionmentioning

confidence: 99%

DNAJC2 is reversely regulated by miR‑627‑3p, promoting the proliferation of colorectal cancer

Liu

Zhao

et al. 2021

Mol Med Rep

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Colorectal cancer (CRC) is one of the most common malignancies among human, which is often connected with increased incidence and mortality rate. DnaJ Heat Shock Protein Family (Hsp40) Member C2 (DNAJC2) is an epigenetic factor, which is involved in a number of cytological functions, such as transcriptional regulation and ubiquitination. A number of studies reveal that DNAJC2 is closely associated with several tumors. However, the function and mechanism of DNAJC2 in CRC remains to be elucidated. In the present study, the expression of DNAJC2 was detected in CRC tissues and adjacent normal tissues. The results indicated that DNAJC2 was increased in CRC tissues and the expression level of DNAJC2 was significantly associated with tumor size. Cell function was detected via Cell Counting Kit-8, 5-ethynyl-2'-deoxyuridine, colony formation assays and flow cytometry by upregulating or knocking down of DNAJC2. Overexpression of DNAJC2 could accelerate cell proliferation while suppression of DNAJC2 decreased cell proliferation, possibly via the regulation of cell cycle regulation in vitro. It was also found that the function of DNAJC2 was reversely regulated by miR-672-3p, causing the promoting of cell proliferation through the activation of AKT/P21 signal pathway in CRC cells. These results suggested that DNAJC2 is a tumor-regulated protein in the progression of CRC and may represent a novel target for CRC detection and therapy.

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<p>MiR-195-5p Inhibits Malignant Progression of Cervical Cancer by Targeting YAP1</p>

Cited by 50 publications

References 40 publications

Circular RNA circ_0000228 promotes the malignancy of cervical cancer via microRNA-195-5p/ lysyl oxidase-like protein 2 axis

Circular RNA circ_0000228 promotes the malignancy of cervical cancer via microRNA-195-5p/ lysyl oxidase-like protein 2 axis

miR‑497 inhibits proliferation and invasion in triple‑negative breast cancer cells via YAP1

DNAJC2 is reversely regulated by miR‑627‑3p, promoting the proliferation of colorectal cancer

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