DNAJC2 is reversely regulated by miR‑627‑3p, promoting the proliferation of colorectal cancer

Liu, Hongfu; Li, Juan; Zhao, Huali; Liu, Xiaohui; Ye, Xinying

doi:10.3892/mmr.2021.12228

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…Studies on the expression of miRNAs in the development of CRC and their regulatory mechanisms have yielded some significant results in recent years. MiRNAs are differentially expressed in tumor tissues, and can either upregulate or downregulate target genes as proto-oncogenes or oncogenes ( 20 , 21 ) we found that miR-17-5p expression was upregulated in colorectal cancer tissues and was significantly correlated with TNM stage and tumor differentiation, tumor diameter and the presence of lymph node metastasis, and had an effect on proliferation, metastasis and invasion and apoptosis of colorectal cancer cells. In addition, we also identified CD44, BCL2, BAX, E-Cadherin, and MMP2 genes as the mechanisms of miR-17-5p regulation in colorectal cancer.…”

Section: Discussionmentioning

confidence: 83%

Expression and clinical significance of miR-17-5p in tumor tissues of patients with colorectal cancer

Liu¹,

Ji²,

Jin³

et al. 2022

J Gastrointest Oncol

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Background: At present, there is a lack of novel biomarkers for the early diagnosis and targeted therapy of colorectal cancer (CRC). The current study investigated the expression of miR-17-5p in colorectal tumors and adjacent tissues, and examined the effects of miR-17-5p on the cellular growth of the colon cancer cell line HCT-116.Methods: A total of 30 paired tissue specimens were obtained from patients with CRC who were admitted to the Department of General Surgery V of the First Affiliated Hospital, Gannan Medical College between December 2019 and January 2021. The clinical information for the corresponding patients was collated.Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was performed to detect the expression of miR-17-5p in tumor tissues and the paracancerous tissues. The relationship between miR-17-5p and clinicopathology was analyzed. The CRC cell line HCT-116 was transfected with miR-17-5p mimics and inhibitors using liposomes. The effects of miR-17-5p on cell proliferation, invasion and migration, and apoptosis were determined using colon formation assays, transwell and scratch assays, and flow cytometry, respectively. The effects of miR-17-5p on CD44 (homing cell adhesion molecule), MMP2 (matrix metalloproteinase 2), BCL2 (B-cell lymphoma-2), BAX (BCL2-associated X), and E-cadherin protein and gene expression were investigated using Western blot and RT-qPCR, respectively.Results: MiR-17-5p expression was higher in tumor tissues compared to the normal adjacent tissues.While miR-17-5p expression levels were unrelated to age nor gender, they were related to the degree of differentiation, stage, lymph node metastasis, and tumor size in patients with CRC. Upregulation of miR-17-5p promoted CRC cell proliferation, metastasis, and invasion, while inhibiting apoptosis. However, downregulation of miR-17-5p had the opposite effect. Furthermore, miR-17-5p upregulation increased E-cadherin, CD44, MMP2, and BCL2 expression while decreasing BAX expression, whereas miR-17-5p downregulation had the opposite effect.Conclusions: MiR-17-5p is highly expressed in tumor tissues and correlates with increased proliferation, invasion, and migration, as well as reduced apoptosis in HCT-116 cells. Indeed, miR-17-5p may be a potential indicator for the early diagnosis of CRC and may guide clinical targeted therapy.

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Section: Discussionmentioning

confidence: 83%

Expression and clinical significance of miR-17-5p in tumor tissues of patients with colorectal cancer

Liu¹,

Ji²,

Jin³

et al. 2022

J Gastrointest Oncol

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“…DnaJ Heat Shock Protein Family (Hsp40) Member C2 (DNAJC2), also known as zuotin-related factor 1, was a recently identi ed epigenetic regulator of gene transcription in stem cells and cancer [32]. High copy ampli cation of the gene has been reported to be involved in the progression of multiple types of cancer, such as prostate cancer, germ cell carcinoma, glioblastoma, head and neck squamous cell carcinoma, colorectal cancer, and gastric cancer [33][34][35]. These data suggested that DNAJC2 is signi cantly related to the proliferation and migration of tumors.…”

Section: Discussionmentioning

confidence: 99%

Identification of a chromatin regulator signature and potential candidate drugs for hepatocellular carcinoma

Mao

Tang

2023

Preprint

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Hepatocellular carcinoma (HCC) is a cancerous tumor that has an unfavorable prognosis. The involvement of chromatin regulators (CRs) in the development of cancer is now supported by a growing body of research. Therefore, we aimed at investigate the function and prognostic importance of CRs in HCC patients. From the prior outstanding research, chromatin regulators (CRs) were obtained. The mRNA expression and clinical data were acquired from the TCGA database. Utilizing Cox regression analysis and least absolute shrinkage and selection operator (LASSO) regression analysis, a risk model for predicting the outcome of HCC was created using the prognostic gene. The Kaplan-Meier analysis was conducted in order to compare the prognosis between high-risk and low-risk groups. We also looked into the differences in drug sensitivity between high-risk and low-risk groups. To estimate prospective small molecule drug therapy, the CMAP dataset was employed. A 13 CRs-based model for predicting the prognosis of HCC patients was effectively built and verified. Furthermore, we discovered that the 13 CRs-based model was a standalone prognostic factor. Functional analysis suggested that the majority of the signaling pathways involved in cancer were enriched in CRs. The immune checkpoint and immune cell infiltration were also associated with the CR-based model. Several medications, including Docetaxel, DMOG, Dasatinib, Axitinib, and Vorinostat, were more sensitive for patients in the high-risk category. Eight small molecule drugs could be beneficial in the treatment of people with HCC. As a result, our research offered novel perspectives into the function of CRs in HCC. We identified a trustworthy prognostic biomarker for the survival of HCC patients.

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“…Liu et al reported DNAJC2 as a diagnostic marker for CRC. They found the high expression of DNAJC2 in tissue samples and cell lines, whereas the knockdown of DNAJC2 via miR 672 3p suppressed the cell growth (Liu et al 2021). ZMYND19 is a protein with a zinc finger domain that enables it to bind to the melanin hormone receptor.…”

Section: Discussionmentioning

confidence: 99%

Identification of ZMYND19 as a novel biomarker of colorectal cancer: RNA-sequencing and machine learning analysis

Khalili-Tanha

Mohit

Asadnia

et al. 2023

J. Cell Commun. Signal.

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Colorectal cancer (CRC) is the third most common cause of cancer-related deaths. The five-year relative survival rate for CRC is estimated to be approximately 90% for patients diagnosed with early stages and 14% for those diagnosed at an advanced stages of disease, respectively. Hence, the development of accurate prognostic markers is required. Bioinformatics enables the identification of dysregulated pathways and novel biomarkers. RNA expression profiling was performed in CRC patients from the TCGA database using a Machine Learning approach to identify differential expression genes (DEGs). Survival curves were assessed using Kaplan-Meier analysis to identify prognostic biomarkers. Furthermore, the molecular pathways, protein-protein interaction, the co-expression of DEGs, and the correlation between DEGs and clinical data have been evaluated. The diagnostic markers were then determined based on machine learning analysis. The results indicated that key upregulated genes are associated with the RNA processing and heterocycle metabolic process, including C10orf2, NOP2, DKC1, BYSL, RRP12, PUS7, MTHFD1L, and PPAT. Furthermore, the survival analysis identified NOP58, OSBPL3, DNAJC2, and ZMYND19 as prognostic markers. The combineROC curve analysis indicated that the combination of C10orf2 -PPAT-ZMYND19 can be considered as diagnostic markers with sensitivity, specificity, and AUC values of 0.98, 1.00, and 0.99, respectively. Eventually, ZMYND19 gene was validated in CRC patients. In conclusion, novel biomarkers of CRC have been identified that may be a promising strategy for early diagnosis, potential treatment, and better prognosis.

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DNAJC2 is reversely regulated by miR‑627‑3p, promoting the proliferation of colorectal cancer

Cited by 8 publications

References 21 publications

Expression and clinical significance of miR-17-5p in tumor tissues of patients with colorectal cancer

Expression and clinical significance of miR-17-5p in tumor tissues of patients with colorectal cancer

Identification of a chromatin regulator signature and potential candidate drugs for hepatocellular carcinoma

Identification of ZMYND19 as a novel biomarker of colorectal cancer: RNA-sequencing and machine learning analysis

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