&lt;p&gt;Long Non-Coding RNA EGOT Promotes the Malignant Phenotypes of Hepatocellular Carcinoma Cells and Increases the Expression of HMGA2 via Down-Regulating miR-33a-5p&lt;/p&gt;

Wu, Shimin; Ai, Hongwu; Zhang, Kehui; Huang, Yun; Xie, Fei

doi:10.2147/ott.s218308

Cited by 16 publications

(11 citation statements)

References 39 publications

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“…13 Some studies revealed that miR-33a-5p regulated metastasis and apoptosis in various cancers. 14,15 However, there are few studies on the regulation of GC mediated by miR-33a-5p. Cyclin-dependent kinases 7 (CDK7) mainly takes part in Pol II transcription process by acting as enzymatic cofactors.…”

Section: Introductionmentioning

confidence: 99%

<p>Gambogic Acid Inhibits the Progression of Gastric Cancer via circRNA_ASAP2/miR-33a-5p/CDK7 Axis</p>

Lin¹,

Lin²,

He³

et al. 2020

CMAR

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Background: Gastric cancer (GC) is a major cancer-related mortality disease. Gambogic acid (GA) has been investigated to inhibit cancer progression. In the present study, the molecular mechanism of GA in regulating GC progression was studied. Methods: The expression levels of circular RNA ASAP2 (circ_ASAP2), miR-33a-5p and cyclin-dependent kinases 7 (CDK7) were detected by quantitative real-time polymerase reaction (qRT-PCR). CDK7 protein level was evaluated by Western blot. Cell colony formation assay, 3-(4,5-Dimethylthazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, transwell assay and flow cytometry analysis were employed to reveal the functional effects among circ_ASAP2, miR-33a-5p and CDK7 on GA-induced GC progression. Mechanistically, the binding relationship between miR-33a-5p and circ_ASAP2 or CDK7 was predicted with starBase v3.0 online database and verified by dual-luciferase reporter assay. In vivo tumor formation assay was used to explain the impacts of GA treatment on GC growth in vivo. Results: Circ_ASAP2 and CDK7 expression were downregulated in GA-induced GC cells compared with GC cells. MiR-33a-5p expression was upregulated in GA-induced GC cells relative to GC cells. The protein expression level of CDK7 was lower in GA-induced GC cells than that in GC cells. Further, circ_ASAP2 overexpression decreased GA-induced inhibition effects on cell proliferation, migration and invasion and GA-induced promotion effect on cell apoptosis in both AGS and HGC-27 cells, whereas this phenomenon was reversed by miR-33a-5p. In addition, circ_ASAP2 functioned as a sponge of miR-33a-5p and miR-33a-5p was associated with CDK7. Furthermore, GA treatment inhibited GC growth in vivo. Conclusion: Circ_ASAP2 overexpression promoted cell proliferation, migration and invasion, whereas inhibited cell apoptosis by upregulating CDK7 expression through binding to miR-33a-5p in GA-induced GC cells. This study provided a theoretical basis in GC treatment with GA.

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Section: Introductionmentioning

confidence: 99%

<p>Gambogic Acid Inhibits the Progression of Gastric Cancer via circRNA_ASAP2/miR-33a-5p/CDK7 Axis</p>

Lin¹,

Lin²,

He³

et al. 2020

CMAR

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“…Furthermore, low miR-33a expression is associated with a low survival rate in patients with hepatocellular carcinoma (33). Similarly, Wu et al (34) and Han et al (35) have illustrated that lncRNA eosinophil granule ontogeny transcript and lncRNA colorectal neoplasia differentially expressed in hepatoma cells inhibit miR-33a expression, which upregulates the target gene high mobility group AT-hook 2 (HMGA2), thereby promoting the proliferation, migration, invasion and chemoresistance of hepatoma cells (Fig. 1E).…”

Section: Mir-33a and Its Role In Cancermentioning

confidence: 72%

Role of microRNA‑33a in malignant cells (Review)

et al. 2020

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Cancer causes most of the mortality and morbidity worldwide, with a significant increase in incidence during recent years. MicroRNAs (miRNAs/miRs) are non-coding small RNAs capable of regulating gene expression. They regulate crucial cellular processes, including proliferation, differentiation, metastasis and apoptosis. Therefore, abnormal miRNA expression is associated with multiple diseases, including cancer. There are two types of cancer-associated miRNAs, oncogenic and tumor suppressor miRNAs, depending on their roles and expression patterns in cancer. Accordingly, miRNAs are considered to be targets for cancer prevention and treatment. miR-33a controls cellular cholesterol uptake and synthesis, which are both closely associated with carcinogenesis. The present review thoroughly describes the roles of miR-33a in more than a dozen types of cancer and the underlying mechanisms. Accordingly, the present review may serve as a guide for researchers studying the involvement of miR-33a in diverse cancer settings. Contents 1. Introduction 2. miR-33a and its role in cancer 3. Discussion 4. Conclusions

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“…Studies by H, L, et al [20]demonstrated that the long-chain non-coding RNA HIF1A-AS2 was overexpressed in osteosarcoma tissues and cells, which could interact with miR-33b-5p and negatively regulate its expression, thereby up-regulating the expression of the target protein SIRT6 of miR-33b-5p. Studies by S, W et al [21]showed that the expression of long-chain non-coding RNA EGOT in hepatocellular carcinoma (HCC) tissues was signi cantly up-regulated. EGOT has a signi cant regulatory effect on the survival, migration and invasion of liver cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Development and Validation of A Seven-microRNA Signature as a Novel Prognostic Biomarker for Bladder Cancer

Lin

et al. 2020

Preprint

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Background The differential expression of miRNAs has played a significant role in bladder tumors. The aim of our study was to screen new biomarkers . Methods Through differential analysis of bladder cancer mRNA and miRNA expression data in the TCGA, differential genes and miRNAs were screened. Furthermore, Cox univariate analysis and multifactor analysis were used to establish a prognostic prediction model . The predictive ability of the prognostic model was then verified on the patient. The action mechanism of these miRNAs was analyzed.Results By the differential analysis and standardization of miRNA expression profiles. Differentially expressed miRNAs were screened, then all the patients were then randomly divided into train group and the test group. 23 miRNAs were revealed , then a Seven-miRNA signature prognostic biomarkers was constituting.Univariate cox regression and multivariate cox regression considering other clinical factors displayed that the seven-miRNA signature could serve as an independent prognostic factor.Target genes of these seven miRNAs were analyzed by KEGG signaling pathway and GO enrichment analysis. . Conclusion The prognostic model constructed by seven miRNAs has possessed certain degree of sensitivity and specificity for the prediction of the survival of bladder cancer patients, which can be used as a potential new clinical marker for bladder cancer patients.

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<p>Long Non-Coding RNA EGOT Promotes the Malignant Phenotypes of Hepatocellular Carcinoma Cells and Increases the Expression of HMGA2 via Down-Regulating miR-33a-5p</p>

Cited by 16 publications

References 39 publications

<p>Gambogic Acid Inhibits the Progression of Gastric Cancer via circRNA_ASAP2/miR-33a-5p/CDK7 Axis</p>

<p>Gambogic Acid Inhibits the Progression of Gastric Cancer via circRNA_ASAP2/miR-33a-5p/CDK7 Axis</p>

Role of microRNA‑33a in malignant cells (Review)

Development and Validation of A Seven-microRNA Signature as a Novel Prognostic Biomarker for Bladder Cancer

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