Development and Validation of A Seven-microRNA Signature as a Novel Prognostic Biomarker for Bladder Cancer

Abstract

Background The differential expression of miRNAs has played a significant role in bladder tumors. The aim of our study was to screen new biomarkers . Methods Through differential analysis of bladder cancer mRNA and miRNA expression data in the TCGA, differential genes and miRNAs were screened. Furthermore, Cox univariate analysis and multifactor analysis were used to establish a prognostic prediction model . The predictive ability of the prognostic model was then verified on the patient. The action mechanism of these miRNAs was analyzed.Results By the differential analysis and standardization of miRNA expression profiles. Differentially expressed miRNAs were screened, then all the patients were then randomly divided into train group and the test group. 23 miRNAs were revealed , then a Seven-miRNA signature prognostic biomarkers was constituting.Univariate cox regression and multivariate cox regression considering other clinical factors displayed that the seven-miRNA signature could serve as an independent prognostic factor.Target genes of these seven miRNAs were analyzed by KEGG signaling pathway and GO enrichment analysis. . Conclusion The prognostic model constructed by seven miRNAs has possessed certain degree of sensitivity and specificity for the prediction of the survival of bladder cancer patients, which can be used as a potential new clinical marker for bladder cancer patients.