&lt;p&gt;Gambogic Acid Inhibits the Progression of Gastric Cancer via circRNA_ASAP2/miR-33a-5p/CDK7 Axis&lt;/p&gt;

Lin, Dan; Lin, Xiaoyang; He, Tianlin; Xie, Guoqun

doi:10.2147/cmar.s269768

Cited by 19 publications

(17 citation statements)

References 32 publications

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“…110 This review summarizes the mechanism of circRNAs involved in tumor cell cycle regulation and finds that their abnormal expression in tumors not only plays a role in tumor proliferation but also in tumor invasion and metastasis, TNM staging, histological grade, malignant phenotype, sensitivity to chemotherapy drugs and patient survival. 3,5,8,9,[25][26][27][28][29][30][31][32][33][111][112][113][114] These findings highlight potential biomarkers for tumor diagnosis and provide an effective reference for developing future tumor treatments involving the regulation of the cell cycle based on circRNA.…”

Section: Discussionmentioning

confidence: 86%

Circular RNA Controls Tumor Occurrence and Development via Cell Cycle Regulation

Liu¹,

Qu²,

Yang³

et al. 2022

OTT

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Circular RNAs (circRNAs) participate in the occurrence and development of various diseases through different mechanisms, such as by acting as a microRNA (miRNA) sponge, interacting with RNA-binding proteins, and regulating gene transcription and protein translation. For example, the abnormal expression of specific circRNAs in tumor cells can alter key regulatory factors and the cell cycle network, resulting in cell cycle disorders and the development and metastasis of tumors. Here, we summarize the mechanisms involved in the circRNA-mediated processes that lead to uncontrolled cell cycle and tumor cell proliferation. Extensive studies investigating the abnormal expression of circRNAs in different cancer types have been conducted. The unique characteristics of circRNAs and their ability to regulate the cell cycle through diverse mechanisms is extremely valuable in tumor diagnosis, treatment, and prognosis. Our review may assist in further understanding the circRNA-mediated regulation of the cell cycle in tumors and provide insights for research on circRNA-based therapeutic strategies and biological diagnosis for cancer.

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Section: Discussionmentioning

confidence: 86%

Circular RNA Controls Tumor Occurrence and Development via Cell Cycle Regulation

Liu¹,

Qu²,

Yang³

et al. 2022

OTT

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“…3 a. As a previous study has indicated that circRNA_ASAP2 (has_circ_0006089) was associated with the progression of GC [ 26 ], further experiments were conducted to investigate the involvement of circRNA_ASAP2 in As4S4-modulated biological behavior alterations in GC cells. Moreover, the expression of circRNA_ASAP2 was significantly decreased in GC cells treated with 1 and 2 μM As 4 S 4 (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Second, the proliferation, migration and invasion were significantly promoted in GC cells after the transfection with pc-circRNA_ASAP2, but these effects were abolished by As 4 S 4 treatment. Similarly, a recent study has also revealed the involvement of circRNA_ASAP2 in the regulation of tumor growth and metastasis in GC [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, impaired levels of circRNAs have been detected in different types of cancer, which are associated with the growth and migration of tumor cells [22][23][24][25]. Furthermore, a recent study has indicated that circRNA_ASAP2 is involved in the development of GC [26]. However, the detailed functions and downstream signaling of circRNAs in the pathogenesis of GC are still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Arsenic sulfide inhibits the progression of gastric cancer through regulating the circRNA_ASAP2/Wnt/β-catenin pathway

Deng

et al. 2021

Anti-Cancer Drugs

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In our paper, the effects of As 4 S 4 treatments on the growth and migration of gastric cancer (GC) cells were explored, and the potential underlying molecular mechanisms were also identified. Cell viability was evaluated by cell counting kit 8 assay. The expression of Ki-67 was examined using immunofluorescence staining. Cell apoptosis was assessed by flow cytometry. The migratory and invasion abilities of cells were determined using Transwell assay. The mRNA and protein levels of related gene were examined by RT-qPCR and western blotting, respectively. CircRNAs chip was performed to identify the differentiated expression of circRNAs in GC cells following the treatment with As 4 S 4 . Our results revealed that the proliferation, migration and invasion of GC cells were remarkably suppressed by the treatment with As 4 S 4 , while cell apoptosis was promoted. Furthermore, circRNA_ASAP2 was a novel target of As 4 S 4 in GC, and it is involved in As 4 S 4 -modulated biological behavior alterations in GC cells. In addition, the activities of the Wnt/β-catenin signaling in GC cells were affected by the overexpression circRNA_ASAP2 and the treatment with As 4 S 4. Moreover, the behavior changes in GC cells caused by the knockdown of circRNA_ASAP2 were reversed by the treatment with Wnt agonist SKL2001. In summary, As 4 S 4 could function as an antitumor agent in GC through regulating the circRNA_ASAP2/Wnt/β-catenin pathway, which in turn influences the growth and metastasis of GC cells.

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“…Furthermore, Dan Lin et al and Qiong Wu et al explored that the expression of miR-33a-5p was descending in GC. However, how it regulates drug resistance of target genes was rarely studied [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

miR-33a-5p Targets RAP2A to Mediate the Sensitivity of Gastric Cancer Cells to 5-FU

et al. 2022

Disease Markers

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Objective. The objective of this study is to explore the effects of microRNA-33a-5p (miR-33a-5p)-ras-related protein Rap-2a (RAP2A) on biological functions of gastric cancer (GC) and to find the potential functional mechanism. Methods. We measured the miR-33a-5p expression in 30 GC tissues and cellular level and 30 adjacent normal tissues as control. Besides, the expression of miR-33a-5p was checked at cell level as well. To screen the possible targets of miR-33a-5p, prediction software was used and gene RAP2A attracted our attention. Through a series of experiments including real-time polymerase chain reaction (qRT-PCR), luciferase assay, and western blotting (WB), we verified RAP2A as a potential target of miR-33a-5p. The impacts of miR-33a-5p and RAP2A on biological functions of GC cell lines (BGC-823 and MGC-803) were analyzed by subsequent experiments. Cell invasion was tested by invasion assays. Cell proliferation was measured by cell counting kit-8 (CCK-8) assay. Cell clone was measured by clone formation assays. Finally, the expression of RAP2A protein was analyzed by WB assay. Results. We found miR-33a-5p was expressed lowly in GC tissues and cells. Overexpression of miR-33a-5p in BGC-823 and MGC-803 cells greatly inhibited the cell invasion and colony number. Furthermore, compared to sh-control (shControl), RAP2A knockdown (sh-RAP2A/shRAP2A) raised the sensitivity of GC cells to 5-FU significantly, characterized as reducing cell apoptosis. Conclusions. The expression of miR-33a-5p was lower in GC cell lines and tissues obviously, indicating that miR-33a-5p served as the antitumor gene in GC. The expression of RAP2A regulated negatively the sensitivity of GC cells to 5-FU. According to our in vitro experiments, miR-33a-5p/RAP2A was likely to become a new therapeutic target for GC.

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<p>Gambogic Acid Inhibits the Progression of Gastric Cancer via circRNA_ASAP2/miR-33a-5p/CDK7 Axis</p>

Cited by 19 publications

References 32 publications

Circular RNA Controls Tumor Occurrence and Development via Cell Cycle Regulation

Circular RNA Controls Tumor Occurrence and Development via Cell Cycle Regulation

Arsenic sulfide inhibits the progression of gastric cancer through regulating the circRNA_ASAP2/Wnt/β-catenin pathway

miR-33a-5p Targets RAP2A to Mediate the Sensitivity of Gastric Cancer Cells to 5-FU

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