<p>LncRNA LINC00689 Promotes the Progression of Gastric Cancer Through Upregulation of ADAM9 by Sponging miR-526b-3p</p>

Abstract

Introduction: Increasing studies have demonstrated that noncoding RNAs, including miRNAs and lncRNAs, have vital roles in mediating cancer progression. However, the expression features and biological functions of LINC00689 in gastric cancer (GC) remain largely unknown. This study was designed to investigate the functions of LINC00689, miR-526b-3p and ADAM9 as well as their interactions in GC. Methods: Real time PCR(RT-PCR) was used to detect the expression of LINC0068, miR-526b-3p and ADAM9 in both GC tissues or cell lines. Gain-and loss-of functions of assays were conducted to verify the role of LINC0068, miR-526b-3p and ADAM9 in GC development. Cell proliferation were determined by CCK8 assay and transwell assay and scratch wound-healing assay were used to test cell invasion and migration. Further, the relationships between LINC00689 and miR-526b-3p, miR-526b-3p and ADAM9 were predicted by bioinformatics analysis and then proved by Luciferase reporter assay and RNA Immunoprecipitation(RIP) assay. Results: We found that LINC00689 was upregulated in GC tissues and positively correlated with advanced tumor stage and tumor size, while miR-526b-3p was downregulated. Furthermore, gain-and loss-of-function experiments revealed that LINC00689 promoted the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of GC cells, while miR-526b-3p had the opposite effects. The underlying mechanisms indicated that LINC00689 functioned as a competing endogenous RNA (ceRNA) by sponging miR-526b-3p in GC cells. Further investigations confirmed that ADAM9 was a direct target of miR-526b-3p and positively modulated the progression of GC. Conclusion: Our study suggests that LINC00689 functions as a novel oncogenic lncRNA in the development of GC by promoting ADAM9 expression through suppression of miR-526b-3p.

LncRNA DDX11-AS1 Exerts Oncogenic Roles in Glioma Through Regulating miR-499b-5p/RWDD4 Axis

“… 21 For example, LncRNA LINC00689 sponges miR-526b to promote gastric cancer development. 22 LncRNA MT1JP sponges miR-214 to inhibit breast cancer progression. 23 DDX11-AS1 has also been reported to sponge several miRNAs, such as miR-499b-5p, miR-873, and miR-326.…”

miR-526b-3p inhibits lung cancer cisplatin-resistance and metastasis by inhibiting STAT3-promoted PD-L1

“…Furthermore, Zhang R et al proved that miR-526b-3p retarded colon cancer metastasis by suppressing HIF1α [ 23 ]. Besides, the ectopic expression of miR-526b-3p interrupted malignancies by forming complexes with long noncoding RNAs and mRNAs [ 24 – 26 ].…”

Long non-coding RNA (lncRNA) HOXB-AS3 promotes cell proliferation and inhibits apoptosis by regulating ADAM9 expression through targeting miR-498-5p in endometrial carcinoma

“…39,40 Mazzocca et al 29 suggested that ADAM9 may play an essential role for modulating tumorstromal interactions. Yin et al 41 discovered that ADAM9 expression is upregulated in gastric cancer tissue and cells, and lncRNA LINC00689 can promote ADAM9 expression by sponging miR-526b-3p. In our study, we found that overexpression of miR-498-5p inhibits protein expression levels of ADAM9 (Figure 5b).…”