LncRNA DDX11-AS1 Exerts Oncogenic Roles in Glioma Through Regulating miR-499b-5p/RWDD4 Axis

Abstract

Background: Long noncoding RNAs (lncRNA) exert essential functions during tumorigenesis. However, how lncRNAs participate in glioma development remains poorly researched. This study aimed to determine how DDX11-AS1 affects glioma progression. Methods: Gene expression was analyzed by qRT-PCR. Survival rate curve was plotted in 56 glioma patients. Loss-of-function assays were performed to analyze proliferation, migration, and invasion through CCK8, colony formation, and transwell assays. Luciferase assay and RNA pulldown assays were conducted to illustrate the underlying molecular mechanism. Results: DDX11-AS1 expression was upregulated in glioma tissues and cells. DDX11-AS1 overexpression was linked with poor prognostic value. DDX11-AS1 knockdown suppressed proliferation, migration, and invasion while inducing apoptosis. DDX11-AS1 interacted with miR-499b-5p to eliminate it, leading to upregulation of RWDD4 expression. RWDD4 was upregulated in glioma while miR-499b-5p was downregulated. Conclusion: DDX11-AS1 upregulation promotes glioma progression through acting as a competing endogenous RNA for miR-499b-5p to upregulate RWDD4.

Unveiling a Ghost Proteome in the Glioblastoma Non-Coding RNAs

“…It is involved in several signaling pathways such as tumor cell proliferation, migration, and invasion. It acts as an miRNA sponge, targeting in particular miR-499b-5p which is involved in the regulation of the expression of the GBM oncogene RWDD4 ( Zheng et al, 2021 ).…”

Long non-coding RNA DDX11-AS1 promotes the proliferation and migration of glioma cells by combining with HNRNPC

“…The research reports that DDX11-AS1 is highly expressed in the cytoplasm of glioma cells. 25 Moreover, increasing studies have shown that lncRNA regulation of cell function in the cytoplasm depends on proteins. 26 , 27 Therefore, to explore the potential binding proteins of DDX11-AS1, we predicted the binding RBPs of DDX11-AS1 on starBase v.2.0, and the results showed that DDX11-AS1 can bind 43 RBPs.…”

“…find that the DDX11-AS1/mir-499B-5p/RWDD4 axis promotes the proliferation of glioma cells. 25 However, the potential other molecular mechanisms of DDX11-AS1 in glioma are poorly understood.…”

LncRNA DDX11 antisense RNA 1 promotes EMT process of esophageal squamous cell carcinoma by sponging miR-30d-5p to regulate SNAI1/ZEB2 expression and Wnt/β-catenin pathway

“…Recent studies have demonstrated that DDX11-AS1, as a novel lncRNA, played an imperative function in the progression and metastasis in multiple cancers, including glioma [ 47 ], bladder cancer [ 21 , 22 ], gastric cancer [ 17 , 18 ], hepatocellular carcinoma [ 15 ], and so on [ 14 , 19 , 20 , 23 ]. In the current research, the DDX11-AS1 functions, as well as molecular mechanisms in EMT process of ESCC, were further explored.…”