<p>Effects of quercetin, sitagliptin alone or in combination in testicular toxicity induced by doxorubicin in rats</p>

Abstract

ObjectiveThis study aimed to evaluate the effect of quercetin and/or sitagliptin on testicular damage induced by doxorubicin (DOX).MethodologyTwenty-five male Wistar rats, weighing 240±20 g, were randomly divided into five groups as follows: a negative control group; that was treated with 1 mL of 0.9% sodium chloride; a DOX-treated group received Intraperitoneal (I.P.) DOX injection (3 mg/kg); a group treated with quercetin 80 mg/kg + sitagliptin 10 mg/kg + DOX; a group treated with quercetin 80 mg/kg + DOX; and a group treated with sitagliptin 10 mg/kg+ DOX. All treatment were given orally daily for 21 days with I.P. DOX 3 mg/kg injection for the treatment groups at days 8, 10, 12, 15, 17, and 19. On day 22, blood was collected for analysis of testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), glutathione peroxidase (GPx), and total antioxidant capacity (TAOC). The testes were also removed and sent for histopathological examination.ResultsThe study revealed that the combination of quercetin with sitagliptin produced a significant increase in testosterone and FSH levels with a non-significant increase in LH level. This combination also non-significantly decreased the level of ALP and LDH and restored the GPx level with enhancing TAOC.ConclusionThe results suggest quercetin/sitagliptin combination as a promising therapeutic modality for attenuation of DOX-induced testicular toxicity in rats, and the main mechanism involved in such effect could be due to the antioxidant and anti-inflammatory properties of both agents.

Cardioprotective Effect of Quercetin and Sitagliptin in Doxorubicin-Induced Cardiac Toxicity in Rats

“…All animals were randomly divided into five groups; the doses and the route of administrations of each treated group have been chosen depending on the previous study. 20 The groups comprise six animals each as follows:…”

“…All animals were randomly divided into five groups; the doses and the route of administrations of each treated group have been chosen depending on the previous study. 20 The groups comprise six animals each as follows: Group I: Control group: Treated with 1 mL of 0.9% sodium chloride orally by gavage tube on days 8, 10, 12, 15, 17, and 19 from the start of the study. Group II: Doxorubicin (DOX) treated group: Received DOX I.P.injection (3 mg/kg) on days 8, 10, 12, 15, 17, 19 from the start of the study.…”

Sitagliptin and fucoidan prevent apoptosis and reducing ER stress in diabetic rat testes

“…Furthermore, in line with other diabetes studies (Donmez et al., 2014; Khamis et al., 2020), a decrease in cell proliferation indices was determined in the diabetic groups in our study. Moreover, in studies published so far demonstrating the effects of sitagliptin (Ahmed et al., 2019) and fucoidan (Ersoy & Kizilay, 2018; Hu et al., 2010) on testicular tissue, there are no data on their effects on testicular cell proliferation. Therefore, our findings of sitagliptin and fucoidan reducing the antiproliferative effects of diabetes are quite valuable.…”

Protective effect of quercetin compared to silymarin against phenylhydrazine induced anemia

“…Also, Olayinka et al (2014) found that Quercetin administration decrease total bilirubin level comparing to Melphalan-induced oxidative stress in rats. Furthermore, Ahmed et al (2019) reported that level of LDH improved with administration of Quercetin. As Quercetin showed valuable effects on liver damage by improving antioxidant enzyme activity and decreasing pro-oxidant effect.…”