Protective effect of quercetin compared to silymarin against phenylhydrazine induced anemia

Abstract

This study has developed to evaluate the protecting effect of Quercetin in phenylhydrazine (PHZ) induced hemolytic anemia comparing to traditional used antioxidant (Silymarin). For that 45 male white Albino rats were randomly allocated into 5 groups, control group, PHZ group which was injected by PHZ (20 mg/kg b.w, I/P at day 7 th , 8 th and 9 th ), Quercetin + PHZ group (Quercetin, 50 mg/kg b.w per os every day for 16 days), silymarin + PHZ group (Silymarin, 100 mg/kg b.w per os every day for 16 days) and Quercetin group. Serum samples of all groups were collected after 3, 5 and 10 days after injection of PHZ and used for biochemical and oxidative stress analysis. Also, specimens from spleen were collected for histopathological examination. The results of this study showed that, Quercetin ameliorated PHZ effect on total protein, total bilirubin, direct and indirect bilirubin, LDH, iron and ferritin levels. Also, Quercetin improved levels of oxidative stress parameters GSH, SOD and MDA, and had protective effect on tissue of spleen. Therefore, Quercetin had protective effect in experimental PHZ-induced hemolytic anemia. Also, it was more potent antioxidant than Silymarin.