<p>Down-regulation of lncRNA LINC00152 Suppresses Gastric Cancer Cell Migration and Invasion Through Inhibition of the ERK/MAPK Signaling Pathway</p>

Abstract

The aim of this study was to explore the regulatory role and mechanism of long noncoding RNA LINC00152 in gastric cancer (GC) cells. Methods: LINC00152 expression in GC tissues and cells was detected by reverse transcription-polymerase chain reaction (qRT-PCR). MKN45 and MGC-803 cells were selected and assigned into different groups after transfection with si-LINC00152, activated ERK/MAPK signaling pathway (SA), or negative control. Cell proliferation, apoptosis, cycle, migration and invasion were assessed by CCK-8, flow cytometry, Transwell assay and Scratch test, respectively. Western blot analysis was conducted to detect the expression of E-cadherin, N-cadherin and ERK/MAPK signaling pathway protein. Results: Compared with the normal tissues, higher expression of LINC00152 was found in GC tissues and LINC00152 was remarkably correlative with clinical stage and lymphatic metastasis. LINC00152 expression in GC cells was higher than that in GES-1 cells. Compared with the NC group, the cell proliferation rate, cells in G2/M phase, migration and invasion abilities as well as the expression of N-cadherin and pERK -1/2 were significantly decreased, and the expression of E-cadherin, cells in G0/G1 phase and cell apoptosis rate were significantly increased in the si-LINC00152-1 group. ERK/MAPK signaling pathway activator SA could reverse the biological role of LINC00152 in GC cells. Conclusion: These results demonstrated that the interference of LINC00152 expression may inhibit the invasion and migration of GC cells by inhibiting the ERK/MAPK signaling pathway.

<p>The Regulatory Mechanism and Biological Significance of Mitochondrial Calcium Uniporter in the Migration, Invasion, Angiogenesis and Growth of Gastric Cancer</p>

“…Unfortunately, in the early stage of gastric cancer, the rate of lymph node metastasis may exceed 20%. 14 We found that MCU expression was significantly correlated with depth of invasion, lymph metastasis, TNM stage and distant metastasis, indicating that MCU expression could be associated with prognosis of gastric cancer. Intriguingly, our results indicated that MCU expression was significantly higher in omental metastasis than gastric cancer tissues, indicating that MCU expression might contribute to gastric cancer metastasis.…”

<p>Long Noncoding RNA LINC00173 Promotes NUTF2 Expression Through Sponging miR-765 and Facilitates Tumorigenesis in Glioma</p>

“…6,7 For example, lncRNA LINC00152 upregulation promotes gastric cancer growth and metastasis. 8 LncRNA SNHG6 overexpression facilitates lung cancer cell proliferation and metastasis. 9 MiR-340-5p dysregulation promotes tumorigenesis of esophageal squamous cell carcinoma.…”

Long Noncoding RNAs in the Metastasis of Oral Squamous Cell Carcinoma

“…LINC00152 (long intergenic noncoding RNA 152), transcribed from the region between protein-coding genes PLGLB2 (plasminogen like B2) and PLGLB1 (plasminogen like B1), is also known as CYTOR (cytoskeleton regulator RNA). Through interfering with cell signaling pathways such as PI3K/AKT ( 105 ), FSCN1 (fascin actin-bundling protein 1) ( 106 ), and Wnt/β-catenin ( 107 , 108 ), LINC00152 was demonstrated to promote the invasion and metastasis of diverse tumors. At present, research on the function of LINC00152 in OSCC metastasis is also mainly focused on its role of ceRNAs.…”