<p>Long Noncoding RNA LINC00173 Promotes NUTF2 Expression Through Sponging miR-765 and Facilitates Tumorigenesis in Glioma</p>

Abstract

Background: Glioma is one of the leading causes of cancer-related deaths. This study aimed to investigate the function and mechanism of long noncoding RNA (lncRNA) LINC00173 in the regulation of glioma progression. Methods: LINC00173 expression was measured using qRT-PCR. Survival rate was analyzed through Kaplan-Meier method. CCK8, colony formation and EdU assays were performed to measure cell proliferation while transwell was used to determine cell migration and invasion. Luciferase reporter assay was conducted to test RNA interaction. Results: LINC00173 expression was elevated in glioma tissues and cells. LINC00173 high expression predicted poor prognosis. Loss of LINC00173 inhibited proliferation, migration and invasion. LINC00173 interacted with miR-765 to enhance NUTF2 expression. MiR-765 expression was negatively correlated with LINC00173 and NUTF2 in glioma tissues. NUTF2 level was increased in glioma tissues. NUTF2 overexpression rescued the potential of proliferation, migration and invasion in LINC00173-silenced cells. Conclusion: Our research demonstrated that LINC00173 promotes glioma progression through targeting miR-765/NUTF2 axis.

Nuclear Transporting Factor 2 as a Novel Biomarker of Head and Neck Squamous Cell Carcinoma and Associated with T/B Cell Receptor Signaling Pathway

“…BioMed Research International [30]. In tumor research, long noncoding RNA (lncRNA) LINC00173 could promote the development of disease by enhancing NUTF2 expression in glioma [19]. Besides, the level of NUTF2 expression was downregulated and negatively correlated with nuclear size in melanoma tissue [18].…”

“…An experiment in Xenopus laevis cells found that decreased level of NUTF2 may contribute to melanoma development through the regulation of cell nuclear size [18]. On the other hand, in 2020, Du et al found that NUTF2 is a downstream target of LINC00173 and regulates tumorigenesis in glioma; its overexpression awakens the proliferation, migration, and invasion of cells [19]. The association between NUTF2 and HNSCC has not been illustrated in previous studies.…”

Identification of LINC00173 in Myasthenia Gravis by Integration Analysis of Aberrantly Methylated- Differentially Expressed Genes and ceRNA Networks

“…Previous study had found that LINC00173 promotes the proliferation and migration of vascular endothelial cells in lung squamous cell carcinoma by acting as ceRNA to bind with miR-511-5p and regulate VEGFA expression ( Chen et al, 2020 ). LINC00173, which had the highest node degree in the hypomethylation-high expression gene-associated ceRNA network, has been investigated as part of the ceRNA networks of lung cancer ( Chen et al, 2020 ), breast cancer ( Fan et al, 2020 ), and glioma ( Du et al, 2020 ), but its molecular mechanism in autoimmune diseases of the nervous system was still unclear. As a constituent of one of the 15 ABL1-associated ceRNA triples, the lncRNA MALAT1 was shown to be downregulated in MG patients and to act as a ceRNA for miR-338-3p ( Kong et al, 2019 ).…”

A Pan-Cancer Analysis of the Oncogenic Role of Nuclear Transport Factor 2 in Human Cancers

“…It was demonstrated that NUTF2 plays vital roles in the phenotype of eyes and diabetic retinopathy via regulating the nuclear import of Ran proteins and the VEGF signaling pathway (9,10). With respect to cancer, upregulation of NUTF2 was found in glioma tissues and overexpression of NUTF2 promoted migration and proliferation of glioma cells, indicating its oncogenic role in glioma (11). However, the role of NUTF2 in other cancer types is largely unknown.…”