<p>Circ-ELF2 Acts as a Competing Endogenous RNA to Facilitate Glioma Cell Proliferation and Aggressiveness by Targeting MiR-510-5p/MUC15 Signaling</p>

Abstract

Objective: Glioma (GM) is a common type of malignant and aggressive tumor in brain with poor prognosis. Circular RNAs (circRNAs) are well-known regulators in cancer progression. However, its molecular basis in GM remains to be investigated. Materials and Methods: CircRNA microarray was used to detect differentially expressed circRNAs in GM and matched noncancerous tissues. qRT-PCR was applied to detect the expression profile of circ-ELF2 in GM tissue specimens and cell lines. CCK-8, clone formation, AO/EB staining, flow cytometry, wound healing, and transwell assays were performed to identify the functions of circ-ELF2 in GM cells. The distribution of circ-ELF2 was analyzed by RNA-FISH and subcellular fractionation assay. Dual-luciferase reporter assay was applied to verify the predicted binding sites between miR-510-5p and circ-ELF2/MUC15 3ʹ-UTR. Rescue assay was finally conducted to explore whether the oncogenic role of circ-ELF2 was partially attributed to miR-510-5p/MUC15 signaling. Results: We observed that circ-ELF2 was significantly upregulated in GM tissues, which was analyzed by circRNA microarray and qRT-PCR. Upregulation of circ-ELF2 was associated with poor prognosis and high recurrence rate for GM patients after surgery. The collapse of circ-ELF2 caused growth arrest and downregulation of cell migratory and invasive potential of GM cells and promoted cell apoptosis. In contrast, elevated expression of circ-ELF2 led to the opposite effect. Mechanistically, circ-ELF2 acted as a competing endogenous RNA (ceRNA) for miR-510-5p to positive modulate MUC15 expression at posttranscriptional level. Circ-ELF2 upregulated MUC15 by sponging miR-510-5p, thus promoting GM growth and aggressiveness. Conclusion: This study indicates that circ-ELF2/miR-510-5p/MUC15 signaling plays a key role in promoting the occurrence and development of GM.

LINC01087 indicates a poor prognosis of glioma patients with preoperative MRI

“…CCK-8 reagent (Sigma-Aldrich, MO, USA) was employed for cell proliferation detection. After culture of 24, 48, 72, and 96 h, cells (2 × 10 3 cells/well) were appended with CCK-8 solution (10 μL/well), and absorbance at 450 nm was recorded on a microplate reader (Zhang et al 2020 ).…”

WGCNA Analysis Identifies Polycystic Ovary Syndrome-Associated Circular RNAs That Interact with RNA-Binding Proteins and Sponge miRNAs

“…The collapse of circ-ELF2 led to growth arrest and accelerated cell apoptosis. 29 The host genes of our other identified DECs were also revealed to mediate the regulation of hormone synthesis, inflammation or cell apoptosis. Ectopic expression of metastasis associated 1 family member 3 (MTA3, host for hsa_circ_0054275) was reported to promote the cAMP-stimulated progesterone secretion and testosterone production.…”

“…Mechanistically, circ-ELF2 acted as a ceRNA for miR-510-5p to positively modulate the expression of mucin 15 at posttranscriptional level. 29 However, their roles remain not well understood. In this study, we predicted five circRNAs (hsa_circ_0063309, hsa_circ_0054275, hsa_circ_0056196, hsa_circ_0018108, hsa_circ_0070987) could sponge hsa-miR-139-5p to upregulate the expression of CASP2, FZD4 and HMGCR; hsa_circ_0063309/hsa_circ_0054275/hsa_circ_0056196 could regulate the expression of FZD4/TAB2/PEA15/RUNX2 by sponging hsa-miR-217; hsa_circ_0063309 also functioned as a ceRNA for hsa-miR-346 and hsa-miR-421 to influence the expression of FZD4/PEA15/RUNX2 and PAK3/CASP2/RUNX2, respectively; hsa_circ_0056196/hsa_circ_0018108 acted as a ceRNA to regulate HMGCR/FZD4 function by sponging hsa-miR-496.…”

“…Mechanistically, circ-ELF2 acted as a ceRNA for miR-510-5p to positively modulate the expression of mucin 15 at posttranscriptional level. 29 However, their roles remain not well understood. In this study, we predicted five circRNAs (hsa_-circ_0063309, hsa_circ_0054275, hsa_circ_0056196, hsa_circ_0018108, hsa_circ_0070987) could sponge hsa-miR-139-5p to upregulate the expression of CASP2, FZD4 and HMGCR; hsa_circ_0063309/hsa_circ_0054275/hsa_-circ_0056196 could regulate the expression of FZD4/…”

The Effect of MicroRNA-409-3p for Treatment and Response to Tumor Proliferation of Lung Cancer Cell Lines (In Vitro)

“…Research in the field of cancer treatment increased significantly in finding a drug or an effective way to stop tumors with no side effects (Azhar et al, 2021), miRNA which is a new class of small non-coding RNA molecules that pair with the 3′ non-coding region of target mRNA, resulting in the deterioration of the mutated mRNA or inhibition of translation, thus controlling and increasing the level of some other people (Fu et al, 2020;Josson et al, 2014), Cancer is usually caused by mutation, and this mutation causes damage to the cellular gene and depresses the tumor protected gene, such as P53, with an increase in other mutagenic proteins (A Al-Hassany et al, 2021;Fu et al, 2020). It is a miRNA with a tumor suppressor effect discovered in recent years and has been found in different types of tumors, such as gastric cancer , bladder and prostate cancer (Yuan et al, 2019;Zhang et al, 2020). Down-regulation of expression targets a variety of genes to regulate tumor proliferation, invasion, and spreading metastases (Bagherian et al, 2021).…”