&lt;I&gt;FASN&lt;/I&gt; Gene Polymorphism in Indigenous Cattle Breeds of Turkey

Öztabak, Kemal; Gürsel, Feraye Esen; Akiş, Iraz; Ateş, Atila; Yardibi, Hasret; Turkay, Gulhan

doi:10.3409/fb62_1.29

Cited by 4 publications

(7 citation statements)

References 30 publications

(33 reference statements)

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“…FASN rs41919985 in the population studied was polymorphic with two genotypes, namely, AG and GG. Different from the results of this study, in which the AA genotype was not found, a previous study found three genotypes (GG, AG, and AA), but the AA genotype only appears at a low frequency of 0.06 in Turkey Anatolian Red cattle [26] and 0.08 in Chinese Holstein cattle and Fleckvieh bulls [18,27]. The AA genotype was reported at a higher frequency (0.28) in Dutch Holstein cattle [9].…”

Section: Discussioncontrasting

confidence: 99%

“…This process is catalyzed by FASN [27]. SNP rs41919985 is found on exon 39 of the FASN gene [26] and is a missense mutation that results in an amino acid change from threonine (ACC) to alanine (GCC). This mutation likely affects the structure of the substrate-binding site and therefore, may result in differences in enzymatic regulation and different milk compositions [26,27].…”

Section: Discussionmentioning

confidence: 99%

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Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle

et al. 2019

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Aim: The study aimed to identify fatty acid synthase (FASN), LOC514211, and fat mass and obesity-associated (FTO) gene polymorphisms and to investigate their associations with milk traits in an Indonesian-Holstein dairy cow population. Materials and Methods: A total of 100 Indonesian-Holstein cows consisting of 50 heads (0th generation; G0) and 50 heads of their daughters (1st generation; G1) were used. Polymerase chain reaction-restriction fragment length polymorphism was performed to genotype three single nucleotide polymorphisms: rs41919985 in the FASN gene, rs42688595 in the LOC514211 gene, and g.1371T>A in the FTO gene. Results: FASN rs41919985 was associated with milk protein percentage (p<0.05), FTO g.1371T>A was associated with milk fat percentage (p<0.05), and LOC514211 rs42688595 was not associated with any trait (p>0.05). Heterozygote variants showed a higher protein percentage for FASN and the highest fat percentage for FTO. These associations were consistent in the G0 and G1 populations. Conclusion: Our results indicate that the milk protein and fat percentages can be improved by increasing the frequency of the AG genotype of FASN and the AT genotype of FTO, respectively.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle

et al. 2019

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“…As reported in other studies, this SNP has an effect over the fatty acid composition of adipose tissue (Zhang et al, 2008;Li et al, 2012;Oh et al, 2012;Yeon et al, 2013;Oztabak et al, 2014), and on fat deposition, as found in Korean cattle by Oh et al (2018), who commented that this SNP could be a casual mutation for increasing fat deposition.…”

Section: Fatty Acid Profilesupporting

confidence: 59%

“…The FASN g.17924A>G (T2264A), SNP is located in the thioesterase domain of FASN enzyme and made a change from polar, neutral, and hydrophilic to nonpolar, aliphatic, and hydrophobic in this motif, affecting the structure of the substrate binding site and therefore changing the specific activity of thioesterase domain towards C14-acyl ACP (Acyl carrier protein domain), which transfers the growing fatty acid chain throughout the different domains of this enzyme (Oh et al, 2018;Zhang et al, 2008), affecting its affinity with the fatty acid (Oztabak et al, 2014). Therefore, the thioesterase domain determines the carbon chain length of the final product of this enzyme and any change or variation in this domain could affect the fatty acid composition of meat and fat (Zhang et al, 2008).…”

Section: There Is a Direct Relation Between Fasn Activity And Fat Depmentioning

confidence: 99%

A Non-Synonymous Single Nucleotide Polymorphism in FASN Gene Alters FASN Enzyme Activity in Subcutaneous and Intramuscular Adipose Tissue in Holstein Friesian Steers

Cancino-Baier

Muñoz

Quiñones

et al. 2021

Annals of Animal Science

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The FASN enzyme plays a key role in fatty acids synthesis as the main long-chain fatty acid synthesizer. A non-synonymous SNP (single nucleotide polymorphism) (g.17925A>G) located in the thioesterase domain of this enzyme and an effect in fat deposition has been observed, but has not been evaluated in this breed and, moreover, the reason whereby this occurs remains unclear. The objective of this study was to evaluate the effect of this SNP on the activity of FASN enzyme in subcutaneous and intramuscular adipose tissue from Holstein Friesian steers. To achieve this, 196 animals were sampled in a local abattoir, genotyped for the FASN g.17924A>G SNP and characterized for fatty acid profile. Then a sub sample of 20 animals per genotype were selected to extract the total protein from subcutaneous and intramuscular adipose tissue to estimate the FASN enzyme activity. The FASN activity for each genotyped animal was assessed indirectly by measuring the decrease in the absorbance of NADPH at 340 nm by spectrophotometry in a 24 well plate in the presence of Acetyl-CoA, Malonyl-CoA, and NADPH. To assess the impact of SNP induced amino acid changes in FASN protein structure, in-silico simulations were performed. Our results indicated that FASN g.17924A>G SNP induces a change in the enzyme activity in subcutaneous adipose tissue, which is higher when the AA genotype is present and lower in the presence of the AG genotype. The in-silico analysis of the amino acid substitution shows that there was a structural change in the dimeric form of the protein between genotypes. Moreover, the global energy between subunits is lower and more favorable when the AA genotype is present and higher and less favorable for the AG genotype. It was also found that the fatty acid profile of subcutaneous adipose tissue was affected when the AG genotype was present, decreasing the C16:0 fatty acid levels and increasing the C18:0 fatty acid levels. The FASN g.17924A>G SNP alters the FASN enzyme structure and activity, leading to a variation in the fatty acid composition of subcutaneous adipose tissue in Holstein Friesian steers. Implications: This SNP could be considered as a tool to improve the fat deposition or marbling and the fatty acid profile in cattle.

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“…Amplification was performed in the thermocycler (MasterCycler -Eppendorf, USA). Oztabak et al (2014) About 1μl of each PCR product was diluted in 10μl of denaturing buffer (98% formamide, 20mM EDTA, 0.05% bromophenol blue and 0.05% xylene cyanol), denatured at 95°C for 5min and maintained on ice. The total volume was applied onto 12.5% polyacrylamide gel.…”

Section: Methodsmentioning

confidence: 99%

The polymorphisms of genes associated with the profile of fatty acids of sheep

Esteves

Livramento

Paiva

et al. 2019

Arq. Bras. Med. Vet. Zootec.

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The present study aimed to evaluate the occurrence of polymorphisms in Diacylglycerol acyltransferase (DGTA-1 and 2), Fatty acid synthase (FASN), Stearoyl-CoA desaturase (SCD) genes and the Thioesterase domain of FASN (TE-FASN) gene that may be related to the lipid profile. In the experiment, a total of 84 sheep from different genetic groups were used. For the evaluation of the polymorphism of the genes, PCR-Single Strand Conformation Polymorphism (SSCP) technique and subsequent sequencing were used. In DGAT-2 gene, four genotypes were identified with the presence of 6 polymorphisms, with two (c.229T> C; c.255T> C) that resulted into the exchange of phenylalanine by leucine. In FASN gene, two genotypes were identified. In TE-FASN gene, three genotypes and 17 polymorphisms were identified. DGAT-1 and SCD genes did not reveal the occurrence of polymorphism. There was difference in relation to C14: 0, C18: 0 fatty acids and Δ9-desaturase C18 for DGAT-2 gene and of C18: 2ω6t for TE-FASN. There were differences among the genetic groups for C10: 0, C12: 0, C17: 0, C18: 2ω6t, C18: 3ω3, C20: 2, total of ω3, ω3/ω6 and atherogenicity index. There is occurrence of polymorphism of DGAT-2 and TE-FASN genes and these should be further studied in sheep since they revealed influence of the genotypes on the fatty acid profile.

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<I>FASN</I> Gene Polymorphism in Indigenous Cattle Breeds of Turkey

Cited by 4 publications

References 30 publications

Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle

Association of single nucleotide polymorphisms in the fatty acid synthase, LOC514211, and fat mass and obesity-associated genes with milk traits in Indonesian-Holstein dairy cattle

A Non-Synonymous Single Nucleotide Polymorphism in FASN Gene Alters FASN Enzyme Activity in Subcutaneous and Intramuscular Adipose Tissue in Holstein Friesian Steers

The polymorphisms of genes associated with the profile of fatty acids of sheep

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